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Misawa (1997)
Metabolic engineering for the production of carotenoids in non-carotenogenic bacteria and yeastsJ Biotechnol, 59
Krahulec (2010)
Fermentation of mixed glucose-xylose substrates by engineered strains of Saccharomyces cerevisiae: Role of the coenzyme specificity of xylose reductase, and effect of glucose on xylose utilizationMicrob Cell Fact, 9
Liu (2006)
Artemisinin: Current state and perspectives for biotechnological production of an antimalarial drugAppl Microbiol Biotechnol, 72
Tsai (2010)
Surface display of a functional minicellulosome by intracellular complementation using a synthetic yeast consortium and its application to cellulose hydrolysis and ethanol productionAppl Environ Microbiol, 76
Martens (2001)
RNA polymerase II and TBP occupy the repressed CYC1 promoterMol Microbiol, 40
Daniel Gietz (2002)
Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol methodMethod Enzymol, 350
Shao (2009)
DNA assembler, an in vivo genetic method for rapid construction of biochemical pathwaysNucleic Acids Res, 37
Ruohonen (1995)
Modifications to the ADH1 promoter of Saccharomyces cerevisiae for efficient production of heterologous proteinsJ Biotechnol, 39
Partow (2010)
Characterization of different promoters for designing a new expression vector in Saccharomyces cerevisiaeYeast, 27
Hauf (2000)
Simultaneous genomic overexpression of seven glycolytic enzymes in the yeast Saccharomyces cerevisiaeEnzyme Microb Technol, 26
Ho (1989)
Site-directed mutagenesis by overlap extension using the polymerase chain reactionGene, 77
Gatignol (1990)
Cloning of Saccharomyces cerevisiae promoters using a probe vector based on phleomycin resistanceGene, 91
Alper (2005)
Tuning genetic control through promoter engineeringProc Natl Acad Sci USA, 102
Lu (2007)
Shuffling of promoters for mutiple genes to optimize xylose fermentation in an engineered Saccharomyces cerevisiae strainAppl Environ Microbiol, 73
Misawa (1990)
Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coliJ Bacteriol, 172
Nevoigt (2006)
Engineering of promoter replacement cassettes for fine-tuning of gene expression in Saccharomyces cerevisiaeAppl Environ Microbiol, 72
Teste (2009)
Validation of reference genes for quantitative expression analysis by real-time RT-PCR in Saccharomyces cerevisiaeBMC Mol Biol, 10
Kotter (1993)
Xylose fermentation by Saccharomyces cerevisiaeAppl Microbiol Biotechnol, 38
Shimada (2004)
Classification and strength measurement of stationary-phase promoters by use of a newly developed promoter cloning vectorJ Bacteriol, 186
Li (2010)
Overcoming glucose repression in mixed sugar fermentation by co-expressing a cellobiose transporter and a beta-glucosidase in Saccharomyces cerevisiaeMol Biosyst, 6
Dekker (1983)
Bioconversion of hemicellulose: Aspects of hemicellulase production by Trichoderma reesei QM 9414 and enzymic saccharification of hemicelluloseBiotechnol Bioeng, 25
Ro (2006)
Production of the antimalarial drug precursor artemisinic acid in engineered yeastNature, 440
Ma (1987)
Plasmid construction by homologous recombination in yeastGene, 58
Dodd (2009)
Enzymatic deconstruction of xylan for biofuel productionGlob Change Biol Bioenergy, 1
Ogden (1986)
Efficient expression of the Saccharomyces cerevisiae PGK gene depends on an upstream activation sequence but does not require TATA sequencesMol Cell Biol, 6
van Zyl (2007)
Consolidated bioprocessing for bioethanol production using Saccharomyces cerevisiaeBiofuels, 108
West (1987)
GAL1/GAL10 divergent promoter region of Saccharomyces cerevisiae contains negative control elements in addition to functionally separate and possibly overlapping upstream activating sequencesGenes Dev, 1
Hawkins (2008)
Production of benzylisoquinoline alkaloids in Saccharomyces cerevisiaeNat Chem Biol, 4
Bitter (1984)
Expression of heterologous genes in Saccharomyces cerevisiae from vectors utilizing the glyceraldehyde-3-phosphate dehydrogenase gene promoterGene, 32
Runquist (2009)
Expression of the Gxf1 transporter from Candida intermedia improves fermentation performance in recombinant xylose-utilizing Saccharomyces cerevisiaeAppl Microbiol Biotechnol, 82
van Peij (1997)
β-Xylosidase activity, encoded by xlnD, is essential for complete hydrolysis of xylan by Aspergillus niger but not for induction of the xylanolytic enzyme spectrumEur J Biochem, 245
Steen (2010)
Microbial production of fatty-acid-derived fuels and chemicals from plant biomassNature, 463
Cartwright (1994)
Use of β-lactamase as a secreted reporter of promoter function in yeastYeast, 10
Hartner (2008)
Promoter library designed for fine-tuned gene expression in Pichia pastorisNucleic Acids Res, 36
Holland (1978)
Isolation and identification of yeast messenger ribonucleic acids coding for enolase, glyceraldehyde-3-phosphate dehydrogenase, and phosphoglycerate kinaseBiochemistry, 17
Hahn-Hagerdal (2007)
Towards industrial pentose-fermenting yeast strainsAppl Microbiol Biotechnol, 74
Denis (1983)
mRNA levels for the fermentative alcohol dehydrogenase of Saccharomyces cerevisiae decrease upon growth on a nonfermentable carbon sourceJ Biol Chem, 258
Jensen (1998)
Artificial promoters for metabolic optimizationBiotechnol Bioeng, 58
Diderich (1999)
Glucose uptake kinetics and transcription of HXT genes in chemostat cultures of Saccharomyces cerevisiaeJ Biol Chem, 274
Ellis (2009)
Diversity-based, model-guided construction of synthetic gene networks with prediced functionsNat Biotechnol, 27
Reifenberger (1997)
Kinetic characterization of individual hexose transporters of Saccharomyces cerevisiae and their relation to the triggering mechanisms of glucose repressionEur J Biochem, 245
Minami (2008)
Microbial production of plant benzylisoquinoline alkaloidsProc Natl Acad Sci USA, 105
Fujita (2004)
Synergistic saccharification, and direct fermentation to ethanol, of amorphous cellulose by use of an engineered yeast strain codisplaying three types of cellulolytic enzymeAppl Environ Microbiol, 70
Ha (2011)
Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentationProc Natl Acad Sci USA, 108
Den Haan (2007a)
Functional expression of cellobiohydrolases in Saccharomyces cerevisiae towards one-step conversion of cellulose to ethanolEnzyme Microb Technol, 40
Schirmaier (1984)
Identification of two genes coding for the translation elongation factor EF-1 alpha of Saccharomyces cerevisiaeEMBO J, 3
Lai (2005)
Dynamical remodeling of the transcriptome during short-term anaerobiosis in Saccharomyces cerevisiae: Differential response and role of Msn2 and/or Msn4 and other factors in galactose and glucose mediaMol Cell Biol, 25
Mumberg (1995)
Yeast vectors for the controlled expression of heterologous proteins in different genetic backgroundsGene, 156
Gasch (2000)
Genomic expression programs in the response of yeast cells to environmental changesMol Biol Cell, 11
Wen (2010)
Yeast surface display of trifunctional minicellulosomes for simultaneous saccharification and fermentation of cellulose to ethanolAppl Environ Microbiol, 76
Xu (2009)
Perspectives and new directions for the production of bioethanol using consolidated bioprocessing of lignocelluloseCurr Opin Biotechnol, 20
Kwast (2002)
Genomic analyses of anaerobically induced genes in Saccharomyces cerevisiae: Functional roles of Rox1p and other factors in mediating the anoxic responseJ Bacteriol, 184
Ajikumar (2010)
Isoprenoid pathway optimization for taxol precursor overproduction in Escherichia coliScience, 330
Den Haan (2007b)
Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiaeMetab Eng, 9
Moskvina (1998)
A search in the genome of Saccharomyces cerevisiae for genes regulated via stress response elementsYeast, 14
Costa (2001)
Oxidative stress and signal transduction in Saccharomyces cerevisiae: Insights into ageing, apoptosis and diseasesMol Aspects Med, 22
Hammer (2006)
Synthetic promoter libraries-tuning of gene expressionTrends Biotechnol, 24
Fernandes (2010)
Metabolic engineering for improved microbial pentose fermentationBioeng Bugs, 1
Schefe (2006)
Quantitative real-time RT-PCR data analysis: Current concepts and the novel “gene expression's CT difference” formulaJ Mol Med, 84
Poutanen (1988)
Characteristics of Trichoderma reesei β-xylosidase and its use in the hydrolysis of solubilized xylansAppl Microbiol Biotechnol, 28
Siewers (2009)
Heterologous production of non-ribosomal peptide LLD-ACV in Saccharomyces cerevisiaeMetab Eng, 11
Jin (2003)
Optimal growth and ethanol production from xylose by recombinant Saccharomyces cerevisiae require moderate D-xylulokinase activityAppl Environ Microbiol, 69
Szczebara (2003)
Total biosynthesis of hydrocortisone from a simple carbon source in yeastNat Biotechnol, 21
Engels (2008)
Metabolic engineering of taxadiene biosynthesis in yeast as a first step towards taxol (paclitaxel) productionMetab Eng, 10
Chemler (2006)
Biosynthesis of isoprenoids, polyunsaturated fatty acids and flavonoids in Saccharomyces cerevisiaeMicrob Cell Fact, 5
Lynd (2005)
Consolidated bioprocessing of cellulosic biomass: An updateCurr Opin Biotechnol, 16
Saha (2003)
Hemicellulose bioconversionJ Ind Microbiol Biotechnol, 30
Saccharomyces cerevisiae is an important platform organism for synthesis of chemicals and fuels. However, the promoters used in most pathway engineering studies in S. cerevisiae have not been characterized and compared in parallel under multiple conditions that are routinely operated in laboratory and the number of known promoters is rather limited for the construction of large biochemical pathways. Here a total of 14 constitutive promoters from S. cerevisiae were cloned and characterized using a green fluorescent protein (GFP) as a reporter in a 2 µ vector pRS426, under varying glucose and oxygen concentrations. The strengths of these promoters varied no more than sixfold in the mean fluorescence intensity of GFP, with promoter TEF1p being the strongest and promoter PGI1p the weakest. As an example of application for these promoters in metabolic engineering, the genes involved in xylan degradation and zeaxanthin biosynthesis were subsequently cloned under the control of promoters with medium to high strength and assembled into a single pathway. The corresponding construct was transformed to a S. cerevisiae strain integrated with a D‐xylose utilizing pathway. The resulting strain produced zeaxanthin with a titer of 0.74 ± 0.02 mg/L directly from birchwood xylan. Biotechnol. Bioeng. 2012; 109:2082–2092. © 2012 Wiley Periodicals, Inc.
Biotechnology and Bioengineering – Wiley
Published: Aug 1, 2012
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