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EDS1 in tomato is required for resistance mediated by TIR‐class R genes and the receptor‐like R gene Ve

EDS1 in tomato is required for resistance mediated by TIR‐class R genes and the receptor‐like R... In tobacco and other Solanaceae species, the tobacco N gene confers resistance to tobacco mosaic virus (TMV), and leads to induction of standard defense and resistance responses. Here, we report the use of N‐transgenic tomato to identify a fast‐neutron mutant, sun1‐1 (suppressor of N), that is defective in N‐mediated resistance. Induction of salicylic acid (SA) and expression of pathogenesis‐related (PR) genes, each signatures of systemic acquired resistance, are both dramatically suppressed in sun1‐1 plants after TMV treatment compared to wild‐type plants. Application of exogenous SA restores PR gene expression, indicating that SUN1 acts upstream of SA. Upon challenge with additional pathogens, we found that the sun1‐1 mutation impairs resistance mediated by certain resistance (R) genes, (Bs4, I, and Ve), but not others (Mi‐1). In addition, sun1‐1 plants exhibit enhanced susceptibility to TMV, as well as to virulent pathogens. sun1‐1 has been identified as an EDS1 homolog present on chromosome 6 of tomato. The discovery of enhanced susceptibility in the sun1‐1 (Le_eds1‐1) mutant plant, which contrasts to reports in Nicotiana benthamiana using virus‐induced gene silencing, provides evidence that the intersection of R gene‐mediated pathways with general resistance pathways is conserved in a Solanaceous species. In tomato, EDS1 is important for mediating resistance to a broad range of pathogens (viral, bacterial, and fungal pathogens), yet shows specificity in the class of R genes that it affects (TIR‐NBS‐LRR as opposed to CC‐NBS‐LRR). In addition, a requirement for EDS1 for Ve‐mediated resistance in tomato exposes that the receptor‐like R gene class may also require EDS1. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Plant Journal Wiley

EDS1 in tomato is required for resistance mediated by TIR‐class R genes and the receptor‐like R gene Ve

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References (65)

Publisher
Wiley
Copyright
Copyright © 2005 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0960-7412
eISSN
1365-313X
DOI
10.1111/j.1365-313X.2005.02380.x
pmid
15842623
Publisher site
See Article on Publisher Site

Abstract

In tobacco and other Solanaceae species, the tobacco N gene confers resistance to tobacco mosaic virus (TMV), and leads to induction of standard defense and resistance responses. Here, we report the use of N‐transgenic tomato to identify a fast‐neutron mutant, sun1‐1 (suppressor of N), that is defective in N‐mediated resistance. Induction of salicylic acid (SA) and expression of pathogenesis‐related (PR) genes, each signatures of systemic acquired resistance, are both dramatically suppressed in sun1‐1 plants after TMV treatment compared to wild‐type plants. Application of exogenous SA restores PR gene expression, indicating that SUN1 acts upstream of SA. Upon challenge with additional pathogens, we found that the sun1‐1 mutation impairs resistance mediated by certain resistance (R) genes, (Bs4, I, and Ve), but not others (Mi‐1). In addition, sun1‐1 plants exhibit enhanced susceptibility to TMV, as well as to virulent pathogens. sun1‐1 has been identified as an EDS1 homolog present on chromosome 6 of tomato. The discovery of enhanced susceptibility in the sun1‐1 (Le_eds1‐1) mutant plant, which contrasts to reports in Nicotiana benthamiana using virus‐induced gene silencing, provides evidence that the intersection of R gene‐mediated pathways with general resistance pathways is conserved in a Solanaceous species. In tomato, EDS1 is important for mediating resistance to a broad range of pathogens (viral, bacterial, and fungal pathogens), yet shows specificity in the class of R genes that it affects (TIR‐NBS‐LRR as opposed to CC‐NBS‐LRR). In addition, a requirement for EDS1 for Ve‐mediated resistance in tomato exposes that the receptor‐like R gene class may also require EDS1.

Journal

The Plant Journal – Wiley

Published: May 1, 2005

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