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The production of freeze‐dried immobilized cultures of Streptococcus thermophilus and their acidification properties in milk

The production of freeze‐dried immobilized cultures of Streptococcus thermophilus and their... C.P. CHAMPAGNE, N.J. GARDNER, L. SOULIGNAC and J.P. INNOCENT.2000.The aim of this study was to prepare alginate‐immobilized freeze‐dried cultures of Streptococcus thermophilus and to compare the acidifying activities of these rehydrated cultures with classical free cell liquid inoculants. Streptococcus thermophilus BT1 grew in alginate beads and the population reached 1010 cfu g−1 after 6 h incubation. Re‐inoculation of the beads in fresh medium with a further 6 h incubation did not improve the biomass level, but extending the incubation at 42 °C to 24 h caused significant death. The rehydrated immobilized cell technology (ICT) starter contained 13% free cells. In acidifying activity tests, the ICT culture had a similar acidification curve to that of a classical milk‐grown free cell culture, except that it reached lower final pH values. Although the differences between the ICT and liquid cultures were not important, there were significant effects of inoculation level on lag time, maximum acidification rate and on the pH and time at which the acidification rate was at its highest. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Applied Microbiology Wiley

The production of freeze‐dried immobilized cultures of Streptococcus thermophilus and their acidification properties in milk

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References (43)

Publisher
Wiley
Copyright
Copyright © 2000 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1364-5072
eISSN
1365-2672
DOI
10.1046/j.1365-2672.2000.00927.x
Publisher site
See Article on Publisher Site

Abstract

C.P. CHAMPAGNE, N.J. GARDNER, L. SOULIGNAC and J.P. INNOCENT.2000.The aim of this study was to prepare alginate‐immobilized freeze‐dried cultures of Streptococcus thermophilus and to compare the acidifying activities of these rehydrated cultures with classical free cell liquid inoculants. Streptococcus thermophilus BT1 grew in alginate beads and the population reached 1010 cfu g−1 after 6 h incubation. Re‐inoculation of the beads in fresh medium with a further 6 h incubation did not improve the biomass level, but extending the incubation at 42 °C to 24 h caused significant death. The rehydrated immobilized cell technology (ICT) starter contained 13% free cells. In acidifying activity tests, the ICT culture had a similar acidification curve to that of a classical milk‐grown free cell culture, except that it reached lower final pH values. Although the differences between the ICT and liquid cultures were not important, there were significant effects of inoculation level on lag time, maximum acidification rate and on the pH and time at which the acidification rate was at its highest.

Journal

Journal of Applied MicrobiologyWiley

Published: Jan 1, 2000

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