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Activation of S-phase-promoting CDKs in late G1 defines a "point of no return" after which Cdc6 synthesis cannot promote DNA replication in yeast.

Activation of S-phase-promoting CDKs in late G1 defines a "point of no return" after which Cdc6... Downloaded from genesdev.cshlp.org on October 15, 2021 - Published by Cold Spring Harbor Laboratory Press Activation of S-~hase-~romoting: CDKs in late G, definis a "pbint of noUreturn1' after whkh Cdc6 svithesis cannot promote DNA replication in yeast Simonetta Piatti?l4 Thomas ohm," Julie H. cocker,' John F.X. ~iffley,' and Kim Nasmyth Research Institute of Molecular Pathology, A-1030 Vienna, Austria; 'Imperial Cancer Research Fund, Clare Hall 3LD, UK Laboratories, South Mimms, Herts EN6 In eukaryotic cells, DNA replication is confined to a discrete period of the cell cycle and does not usually recur until after anaphase. In the budding yeast Saccharomyces cerevisiae, assembly of pre-replication complexes (pre-RCs) at future origins as cells exit mitosis (or later during G,) is necessary for subsequent initiation of DNA replication triggered by activation in late GI of Cdc28lCdkl kinases associated with B-type cyclins Clbl-Clb6. The absence of pre-RCs during G, and M phases could explain why origins of DNA replication fire only once during the cell cycle, even though S-phase-promoting Cdks remain active from the beginning of S phase through the end of M phase. Formation of pre-RCs and their maintenance during G, depend on the synthesis and activity of an unstable protein encoded by http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genes & Development Unpaywall

Activation of S-phase-promoting CDKs in late G1 defines a "point of no return" after which Cdc6 synthesis cannot promote DNA replication in yeast.

Piatti, S; Böhm, T; Cocker, J H; Diffley, J F; Nasmyth, K
Genes & Development – Jun 15, 1996
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Publisher
Unpaywall
ISSN
0890-9369
DOI
10.1101/gad.10.12.1516
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Abstract

Downloaded from genesdev.cshlp.org on October 15, 2021 - Published by Cold Spring Harbor Laboratory Press Activation of S-~hase-~romoting: CDKs in late G, definis a "pbint of noUreturn1' after whkh Cdc6 svithesis cannot promote DNA replication in yeast Simonetta Piatti?l4 Thomas ohm," Julie H. cocker,' John F.X. ~iffley,' and Kim Nasmyth Research Institute of Molecular Pathology, A-1030 Vienna, Austria; 'Imperial Cancer Research Fund, Clare Hall 3LD, UK Laboratories, South Mimms, Herts EN6 In eukaryotic cells, DNA replication is confined to a discrete period of the cell cycle and does not usually recur until after anaphase. In the budding yeast Saccharomyces cerevisiae, assembly of pre-replication complexes (pre-RCs) at future origins as cells exit mitosis (or later during G,) is necessary for subsequent initiation of DNA replication triggered by activation in late GI of Cdc28lCdkl kinases associated with B-type cyclins Clbl-Clb6. The absence of pre-RCs during G, and M phases could explain why origins of DNA replication fire only once during the cell cycle, even though S-phase-promoting Cdks remain active from the beginning of S phase through the end of M phase. Formation of pre-RCs and their maintenance during G, depend on the synthesis and activity of an unstable protein encoded by

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Genes & Development – Unpaywall

Published: Jun 15, 1996

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