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- Publisher
- Springer Journals
- Copyright
- Copyright © 2008 by Springer-Verlag
- Subject
- Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
- ISSN
- 0304-8608
- eISSN
- 1432-8798
- DOI
- 10.1007/s00705-008-0200-0
- pmid
- 18815724
- Publisher site
- See Article on Publisher Site
Abstract
Dengue-1 viruses responsible for the dengue fever outbreak in Easter Island in 2002 were isolated from acute-phase sera of dengue fever patients. In order to analyze the complete genome sequence, we designed primers to amplify contiguous segments across the entire sequence of the viral genome. RT-PCR products obtained were cloned, and complete nucleotide and deduced amino acid sequences were determined. This report constitutes the first complete genetic characterization of a DENV-1 isolate from Chile. Phylogenetic analysis shows that an Easter Island isolate is most closely related to Pacific DENV-1 genotype IV viruses.
Journal
Archives of Virology – Springer Journals
Published: Oct 1, 2008