Access the full text.
Sign up today, get DeepDyve free for 14 days.
D. Carney, D. Cunningham (1978)
Cell surface action of thrombin is sufficient to initiate division of chick cellsCell, 14
E. Reich (1978)
ACTIVATION OF PLASMINOGEN: A GENERAL MECHANISM FOR PRODUCING LOCALIZED EXTRACELLULAR PROTEOLYSIS
D. Gospodarowicz (1974)
Localisation of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growthNature, 249
H. Soreq, R. Miskin (1981)
Plasminogen activator in the rodent brainBrain Research, 216
J. Unkeless, A. Tobia, L. Ossowski, J. Quigley, D. Rifkin, E. Reich (1973)
AN ENZYMATIC FUNCTION ASSOCIATED WITH TRANSFORMATION OF FIBROBLASTS BY ONCOGENIC VIRUSESThe Journal of Experimental Medicine, 137
J. Bottenstein, S. Skaper, S. Varon, G. Sato (1980)
Selective survival of neurons from chick embryo sensory ganglionic dissociates utilizing serum-free supplemented medium.Experimental cell research, 125 1
R. Morrison, J. Vellis (1981)
Growth of purified astrocytes in a chemically defined medium.Proceedings of the National Academy of Sciences of the United States of America, 78 11
N. Kalderon (1979)
Migration of Schwann cells and wrapping of neurites in vitro: a function of protease activity (plasmin) in the growth medium.Proceedings of the National Academy of Sciences of the United States of America, 76 11
B. Zetter, L. Chen, J. Buchanan (1976)
Effects of protease treatment on growth, morphology, adhesion, and cell surface proteins of secondary chick embryo fibroblastsCell, 7
S. Strickland, W. Beers (1976)
Studies on the role of plasminogen activator in ovulation. In vitro response of granulosa cells to gonadotropins, cyclic nucleotides, and prostaglandins.The Journal of biological chemistry, 251 18
Puck Puck, Cieciura Cieciura, Robinson Robinson (1958)
Genetics of somatic mammalian cells III. Long‐term cultivation of euploid cells from human and animal subjectsJ Exp Med, 108
A. Krystosek, N. Seeds (1981)
Plasminogen activator secretion by granule neurons in cultures of developing cerebellum.Proceedings of the National Academy of Sciences of the United States of America, 78 12
T. Puck, S. Cieciura, A. Robinson (1958)
GENETICS OF SOMATIC MAMMALIAN CELLSThe Journal of Experimental Medicine, 108
Brunner Brunner, Lang Lang, Wolfe Wolfe, Mc Clure Mc Clure, Sato Sato (1981)
Selective cell culture of brain cells by serumfree hormone‐supplemented media: A comparative morphological studyDev Brain Res, 2
D. Carney, D. Cunningham (1977)
Initiation of chick cell division by trypsin action at the cell surfaceNature, 268
L. Ossowski, D. Biegel, E. Reich (1979)
Mammary plasminogen activator: correlation with involution, hormonal modulation and comparison between normal and neoplastic tissueCell, 16
G. Fischbach, M. Dichter (1974)
Electrophysiologic and morphologic properties of neurons in dissociated chick spinal cord cell cultures.Developmental biology, 37 1
G. Brunner, K. Lang, R. Wolfe, D. Mcclure, G. Sato (1981)
Selective cell culture of brain cells by serum-free, hormone-supplemented media: a comparative morphological study.Brain research, 254 4
S. Skaper, R. Adler, S. Varon (1979)
A procedure for purifying neuron-like cells in cultures from central nervous tissue with a defined medium.Developmental neuroscience, 2 5
M. Brown, D. Kiehn (1977)
Protease effects on specific growth properties of normal and transformed baby hamster kidney cells.Proceedings of the National Academy of Sciences of the United States of America, 74 7
Unkeless Unkeless, Tobia Tobia, Ossowski Ossowski, Quigley Quigley, Rifkin Rifkin, Reich Reich (1973)
An enzymatic function associated with transformation of fibroblasts by oncogenic viruses I. Chick embryo fibroblast cultures transformed by avian RNA tumor virusesJ Exp Med, 137
M. Bradford (1976)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical biochemistry, 72
S. Fujita (1965)
An autoradiographic study on the origin and fate of the sub‐pial glioblast in the embryonic chick spinal cordJournal of Comparative Neurology, 124
Kalderon Kalderon (1981)
Role of proteolysis in morphogenesis of developing nervous systemJ Cell Biol, 91
(1981)
Plasminogen activator production and glial cell division: Parallel Krystosek A
J. Quigley (1979)
Phorbol ester-induced morphological changes in transformed chick fibroblasts: Evidence for direct catalytic involvement of plasminogen activatorCell, 17
J. Bottenstein, G. Sato (1979)
Growth of a rat neuroblastoma cell line in serum-free supplemented medium.Proceedings of the National Academy of Sciences of the United States of America, 76 1
D. Barnes, G. Sato (1980)
Methods for growth of cultured cells in serum-free medium.Analytical biochemistry, 102 2
M. Burger (1970)
Proteolytic Enzymes Initiating Cell Division and Escape from Contact Inhibition of GrowthNature, 227
A. Leyva, W. Kelley (1974)
Measurement of DNA in cultured human cells.Analytical biochemistry, 62 1
B. Sefton, H. Rubin (1970)
Release from Density Dependent Growth Inhibition by Proteolytic EnzymesNature, 227
T. Puck (1957)
The genetics of somatic mammalian cells.Advances in biological and medical physics, 5
P. Blumberg, P. Robbins (1975)
Effect of proteases on activation of resting chick embryo fibroblasts and on cell surface proteinsCell, 6
D. Deutsch, E. Mertz (1970)
Plasminogen: Purification from Human Plasma by Affinity ChromatographyScience, 170
S. Strickland, E. Reich, M. Sherman (1976)
Plasminogen activator in early embryogenesis: Enzyme production by trophoblast and parietal endodermCell, 9
The role of the plasmin‐generating system, a serum component, in the development of dissociated embryonic chick spinal cord cells in culture was studied. Studies were performed in a defined system where the cells were maintained in a serum‐free medium. Under these conditions the cells produce plasminogen activator. It was found that plasminogen, when added to the chemically defined culture medium at concentrations of 0.2–0.75 μg/ml, stimulates (3H)thymidine uptake (as expressed per total DNA) in a dose‐response manner. This mitogenic effect is abolished by the protease inhibitors leupeptin and aprotinin. Trypsin, but not chymotrypsin, can produce similar effects. It is concluded that plasmin, which is produced as a result of the activation of plasminogen, is a component that serves as a proliferation factor in developing spinal cords in culture.
Journal of Neuroscience Research – Wiley
Published: Jan 1, 1982
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.