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Environmental regulation of plant gene expression: An RT‐qPCR laboratory project for an upper‐level undergraduate biochemistry or molecular biology course

Environmental regulation of plant gene expression: An RT‐qPCR laboratory project for an... We present a novel laboratory project employing “real‐time” RT‐qPCR to measure the effect of environment on the expression of the FLOWERING LOCUS C gene, a key regulator of floral timing in Arabidopsis thaliana plants. The project requires four 3‐hr laboratory sessions and is aimed at upper‐level undergraduate students in biochemistry or molecular biology courses. The project provides students with hands‐on experience with RT‐qPCR, the current “gold standard” for gene expression analysis, including detailed data analysis using the common 2−ΔΔCT method. Moreover, it provides a convenient starting point for many inquiry‐driven projects addressing diverse questions concerning ecological biochemistry, naturally occurring genetic variation, developmental biology, and the regulation of gene expression in nature. © 2013 by The International Union of Biochemistry and Molecular Biology, 41(5):325–333, 2013 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biochemistry and Molecular Biology Education Wiley

Environmental regulation of plant gene expression: An RT‐qPCR laboratory project for an upper‐level undergraduate biochemistry or molecular biology course

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References (33)

Publisher
Wiley
Copyright
Copyright © 2013 Wiley Periodicals, Inc.
ISSN
1470-8175
eISSN
1539-3429
DOI
10.1002/bmb.20722
pmid
24038665
Publisher site
See Article on Publisher Site

Abstract

We present a novel laboratory project employing “real‐time” RT‐qPCR to measure the effect of environment on the expression of the FLOWERING LOCUS C gene, a key regulator of floral timing in Arabidopsis thaliana plants. The project requires four 3‐hr laboratory sessions and is aimed at upper‐level undergraduate students in biochemistry or molecular biology courses. The project provides students with hands‐on experience with RT‐qPCR, the current “gold standard” for gene expression analysis, including detailed data analysis using the common 2−ΔΔCT method. Moreover, it provides a convenient starting point for many inquiry‐driven projects addressing diverse questions concerning ecological biochemistry, naturally occurring genetic variation, developmental biology, and the regulation of gene expression in nature. © 2013 by The International Union of Biochemistry and Molecular Biology, 41(5):325–333, 2013

Journal

Biochemistry and Molecular Biology EducationWiley

Published: Sep 1, 2013

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