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K. Elkins, Raelynn Kadunc (2012)
An undergraduate laboratory experiment for upper-level forensic science, biochemistry, or molecular biology courses: human DNA amplification using STR single locus primers by real-time PCR with SYBR green detectionJournal of Chemical Education, 89
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We present a novel laboratory project employing “real‐time” RT‐qPCR to measure the effect of environment on the expression of the FLOWERING LOCUS C gene, a key regulator of floral timing in Arabidopsis thaliana plants. The project requires four 3‐hr laboratory sessions and is aimed at upper‐level undergraduate students in biochemistry or molecular biology courses. The project provides students with hands‐on experience with RT‐qPCR, the current “gold standard” for gene expression analysis, including detailed data analysis using the common 2−ΔΔCT method. Moreover, it provides a convenient starting point for many inquiry‐driven projects addressing diverse questions concerning ecological biochemistry, naturally occurring genetic variation, developmental biology, and the regulation of gene expression in nature. © 2013 by The International Union of Biochemistry and Molecular Biology, 41(5):325–333, 2013
Biochemistry and Molecular Biology Education – Wiley
Published: Sep 1, 2013
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