Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/wiley/characterization-of-the-pseudomonas-syringae-pv-tomato-avrrpt2-protein-wmyeP1t4Rt
References (59)
-
Proc Natl Acad Sci -
S. Scofield, C. Tobias, J. Rathjen, Jeff Chang, Daniel Lavelle, R. Michelmore, B. Staskawicz (1996)
Molecular Basis of Gene-for-Gene Specificity in Bacterial Speck Disease of TomatoScience, 274
-
O. Garred, E. Dubinina, P. Holm, S. Olsnes, B. Deurs, J. Kozlov, K. Sandvig (1995)
Role of processing and intracellular transport for optimal toxicity of Shiga toxin and toxin mutants.Experimental cell research, 218 1
-
Vincent Lee, Deborah Anderson, O. Schneewind (1998)
Targeting of Yersinia Yop proteins into the cytosol of HeLa cells: one‐step translocation of YopE across bacterial and eukaryotic membranes is dependent on SycE chaperoneMolecular Microbiology, 28
-
M. Mindrinos, F. Katagiri, Guo-Liang Yu, Frederick Ausubei
The A. Thaliana Disease Resistance Gene Rps2 Encodes a Protein Containing a Nucleotide-binding Site and Leucine-rich Repeats -
A. Charkowski, J. Alfano, G. Preston, J. Yuan, S. He, A. Collmer (1998)
The Pseudomonas syringae pv. tomato HrpW Protein Has Domains Similar to Harpins and Pectate Lyases and Can Elicit the Plant Hypersensitive Response and Bind to PectateJournal of Bacteriology, 180
-
N. Puri, C. Jenner, M. Bennett, R. Stewart, J. Mansfield, N. Lyons, J. Taylor (1997)
Expression of avrPphB, an avirulence gene from Pseudomonas syringae pv. phaseolicola, and the delivery of signals causing the hypersensitive reaction in bean.Molecular plant-microbe interactions : MPMI, 10 2
-
Turner Turner, Barber Barber, Daniels Daniels (1984)
Behaviour of the transposons Tn 5 and Tn 7 in Xanthomonas campestris pv. campestrisMol Gen Genet, 195
-
He, secrete Avr proteins in culture -
K. Schesser, E. Frithz‐Lindsten, H. Wolf‐Watz (1996)
Delineation and mutational analysis of the Yersinia pseudotuberculosis YopE domains which mediate translocation across bacterial and eukaryotic cellular membranesJournal of Bacteriology, 178
-
J. Murillo, H. Shen, D. Gerhold, A. Sharma, D. Cooksey, N. Keen (1994)
Characterization of pPT23B, the plasmid involved in syringolide production by Pseudomonas syringae pv. tomato PT23.Plasmid, 31 3
-
Leister Rt, F. Ausubel, Fumiaki Katagiri (1996)
Molecular recognition of pathogen attack occurs inside of plant cells in plant disease resistance specified by the Arabidopsis genes RPS2 and RPM1.Proceedings of the National Academy of Sciences of the United States of America, 93 26
-
Jihyun Kim, S. Beer (1998)
HrpW of Erwinia amylovora, a New Harpin That Contains a Domain Homologous to Pectate Lyases of a Distinct ClassJournal of Bacteriology, 180
-
T. Michiels, G. Cornelis (1991)
Secretion of hybrid proteins by the Yersinia Yop export systemJournal of Bacteriology, 173
-
G. Ackerveken, E. Marois, U. Bonas (1996)
Recognition of the Bacterial Avirulence Protein AvrBs3 Occurs inside the Host Plant CellCell, 87
-
W. Deng, G. Preston, A. Collmer, C. Chang, Hsiou‐Chen Huang (1998)
Characterization of the hrpC and hrpRSOperons of Pseudomonas syringae Pathovars Syringae, Tomato, and Glycinea and Analysis of the Ability of hrpF,hrpG, hrcC, hrpT, and hrpVMutants To Elicit the Hypersensitive Response and Disease in PlantsJournal of Bacteriology, 180
-
M. Pirhonen, M. Lidell, D. Rowley, S. Lee, S. Jin, Y. Liang, S. Silverstone, N. Keen, S. Hutcheson (1996)
Phenotypic expression of Pseudomonas syringae avr genes in E. coli is linked to the activities of the hrp-encoded secretion system.Molecular plant-microbe interactions : MPMI, 9 4
-
M. Arlat, F. Gijsegem, J. Huet, J. Pernollet, C. Boucher (1994)
PopA1, a protein which induces a hypersensitivity‐like response on specific Petunia genotypes, is secreted via the Hrp pathway of Pseudomonas solanacearum.The EMBO Journal, 13
-
A. Bogdanove, D. Bauer, S. Beer (1998)
Erwinia amylovora Secretes DspE, a Pathogenicity Factor and Functional AvrE Homolog, through the Hrp (Type III Secretion) PathwayJournal of Bacteriology, 180
-
A. Bent, B. Kunkel, D. Dahlbeck, Kit Brown, R. Schmidt, J. Giraudat, J. Leung, B. Staskawicz (1994)
RPS2 of Arabidopsis thaliana: a leucine-rich repeat class of plant disease resistance genes.Science, 265 5180
-
S. He, Hsiou‐Chen Huang, A. Collmer (1993)
Pseudomonas syringae pv. syringae harpinPss: A protein that is secreted via the hrp pathway and elicits the hypersensitive response in plantsCell, 73
-
A. Charkowski, Hsiou‐Chen Huang, A. Collmer (1997)
Altered localization of HrpZ in Pseudomonas syringae pv. syringae hrp mutants suggests that different components of the type III secretion pathway control protein translocation across the inner and outer membranes of gram-negative bacteriaJournal of Bacteriology, 179
-
M. Sory, A. Boland, I. Lambermont, G. Cornelis (1995)
Identification of the YopE and YopH domains required for secretion and internalization into the cytosol of macrophages, using the cyaA gene fusion approach.Proceedings of the National Academy of Sciences of the United States of America, 92 26
-
Xiaoyan Tang, R. Frederick, Jianmin Zhou, D. Halterman, Yulin Jia, G. Martin (1996)
Initiation of Plant Disease Resistance by Physical Interaction of AvrPto and Pto KinaseScience, 274
-
Don Anderson, O. Schneewind (1997)
A mRNA signal for the type III secretion of Yop proteins by Yersinia enterocolitica.Science, 278 5340
-
C. Hueck (1998)
Type III Protein Secretion Systems in Bacterial Pathogens of Animals and PlantsMicrobiology and Molecular Biology Reviews, 62
-
E. Roine, Wensheng Wei, Jing Yuan, E. Nurmiaho‐Lassila, N. Kalkkinen, M. Romantschuk, S. He (1997)
Hrp pilus: an hrp-dependent bacterial surface appendage produced by Pseudomonas syringae pv. tomato DC3000.Proceedings of the National Academy of Sciences of the United States of America, 94 7
-
Koncz Koncz, Schell Schell (1986)
The promoter of T L ‐DNA gene 5 controls the tissue‐specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vectorMol Gen Genet, 204
-
J. Greenberg (1997)
PROGRAMMED CELL DEATH IN PLANT-PATHOGEN INTERACTIONS.Annual review of plant physiology and plant molecular biology, 48
-
T. McNellis, M. Mudgett, Karen Li, Takashi Aoyama, Diana Horvath, N. Chua, B. Staskawicz (1998)
Glucocorticoid-inducible expression of a bacterial avirulence gene in transgenic Arabidopsis induces hypersensitive cell death.The Plant journal : for cell and molecular biology, 14 2
-
Innes Innes, Bent Bent, Kunkel Kunkel, Bisgrove Bisgrove, Staskawicz Staskawicz (1993)
Molecular analysis of avirulence gene avrRpt2 and identification of a putative regulatory sequence common to all known Pseudomonas syringaeJ Bacteriol, 175
-
J. Sambrook, E. Fritsch, T. Maniatis (2001)
Molecular Cloning: A Laboratory Manual -
M. Delledonne, Yiji Xia, R. Dixon, C. Lamb (1998)
Nitric oxide functions as a signal in plant disease resistanceNature, 394
-
R. Innes, A. Bent, B Kunkel, S Bisgrove, B. Staskawicz (1993)
Molecular analysis of avirulence gene avrRpt2 and identification of a putative regulatory sequence common to all known Pseudomonas syringae avirulence genesJournal of Bacteriology, 175
-
D. Figurski, D. Helinski (1979)
Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans.Proceedings of the National Academy of Sciences of the United States of America, 76 4
-
J. Dangl (1994)
The enigmatic avirulence genes of phytopathogenic bacteria.Current topics in microbiology and immunology, 192
-
M. Mudgett, B. Staskawicz (1998)
Protein signaling via type III secretion pathways in phytopathogenic bacteria.Current opinion in microbiology, 1 1
-
Maureen Whalen, R. Innes, A. Bent, B. Staskawicz (1991)
Identification of Pseudomonas syringae pathogens of Arabidopsis and a bacterial locus determining avirulence on both Arabidopsis and soybean.The Plant cell, 3
-
Thanh Huynh, D. Dahlbeck, B. Staskawicz (1989)
Bacterial blight of soybean: regulation of a pathogen gene determining host cultivar specificity.Science, 245 4924
-
(1993)
Techniques in Protein ChemistryJournal of the American Society for Mass Spectrometry, 4
-
Hsiou‐Chen Huang, S. He, D. Bauer, A. Collmer (1992)
The Pseudomonas syringae pv. syringae 61 hrpH product, an envelope protein required for elicitation of the hypersensitive response in plantsJournal of Bacteriology, 174
-
J. Durner, D. Wendehenne, D. Klessig (1998)
Defense gene induction in tobacco by nitric oxide, cyclic GMP, and cyclic ADP-ribose.Proceedings of the National Academy of Sciences of the United States of America, 95 17
-
Suresh Gopalan, D. Bauer, J. Alfano, A. Loniello, S. He, A. Collmer (1996)
Expression of the Pseudomonas syringae avirulence protein AvrB in plant cells alleviates its dependence on the hypersensitive response and pathogenicity (Hrp) secretion system in eliciting genotype-specific hypersensitive cell death.The Plant cell, 8
-
K. Hammond-Kosack, Jonathan Jones (1997)
PLANT DISEASE RESISTANCE GENES.Annual review of plant physiology and plant molecular biology, 48
-
H. Flor (1971)
Current Status of the Gene-For-Gene ConceptAnnual Review of Phytopathology, 9
-
S. Gaudriault, L. Malandrin, Jean-Pierre Paulin, M. Barny (1997)
DspA, an essential pathogenicity factor of Erwinia amylovora showing homology with AvrE of Pseudomonas syringae, is secreted via the Hrp secretion pathway in a DspB‐dependent wayMolecular Microbiology, 26
-
J. Alfano, A. Collmer (1997)
The type III (Hrp) secretion pathway of plant pathogenic bacteria: trafficking harpins, Avr proteins, and deathJournal of Bacteriology, 179
-
A. Matthysse, S. Stretton, C. Dandie, N. McClure, A. Goodman (1996)
Construction of GFP vectors for use in gram-negative bacteria other than Escherichia coli.FEMS microbiology letters, 145 1
-
F. Gijsegem, Stéphane Genin, C. Boucher (1993)
Conservation of secretion pathways for pathogenicity determinants of plant and animal bacteria.Trends in microbiology, 1 5
-
M. Mudgett, Steven Clarke (1994)
Hormonal and environmental responsiveness of a developmentally regulated protein repair L-isoaspartyl methyltransferase in wheat.The Journal of biological chemistry, 269 41
-
C. Lamb, R. Dixon (1997)
THE OXIDATIVE BURST IN PLANT DISEASE RESISTANCE.Annual review of plant physiology and plant molecular biology, 48
-
Zhong-Min Wei, R. Laby, C. Zumoff, D. Bauer, S. He, A. Collmer, S. Beer (1992)
Harpin, elicitor of the hypersensitive response produced by the plant pathogen Erwinia amylovora.Science, 257 5066
-
Jong Ham, David Bauer, D. Fouts, A. Collmer (1998)
A cloned Erwinia chrysanthemi Hrp (type III protein secretion) system functions in Escherichia coli to deliver Pseudomonas syringae Avr signals to plant cells and to secrete Avr proteins in culture.Proceedings of the National Academy of Sciences of the United States of America, 95 17
-
B. Kunkel, A. Bent, D. Dahlbeck, R. Innes, B. Staskawicz (1993)
RPS2, an Arabidopsis disease resistance locus specifying recognition of Pseudomonas syringae strains expressing the avirulence gene avrRpt2.The Plant cell, 5
-
James Morrissey (1981)
Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity.Analytical biochemistry, 117 2
-
U. Laemmli (1970)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 227
-
D. Hanahan, D. Hanahan (1983)
Studies on transformation of Escherichia coli with plasmids.Journal of molecular biology, 166 4
-
A. Bogdanove, Z. Wei, L. Zhao, S. Beer (1996)
Erwinia amylovora secretes harpin via a type III pathway and contains a homolog of yopN of Yersinia sppJournal of Bacteriology, 178
-
Jing Yuan, Shengyang He (1996)
The Pseudomonas syringae Hrp regulation and secretion system controls the production and secretion of multiple extracellular proteinsJournal of Bacteriology, 178
- Publisher
- Wiley
- Copyright
- Blackwell Science Ltd, Oxford
- ISSN
- 0950-382X
- eISSN
- 1365-2958
- DOI
- 10.1046/j.1365-2958.1999.01403.x
- Publisher site
- See Article on Publisher Site
Abstract
Pseudomonas syringae pv. tomato strain DC3000 (Pst DC3000) expressing avrRpt2 is specifically recognized by plant cells expressing RPS2 activity, resulting in localized cell death and plant resistance. Furthermore, transient expression of this bacterial avrRpt2 gene in plant cells results in RPS2‐dependent cell death. This indicates that the AvrRpt2 protein is recognized inside RPS2 plant cells and is sufficient for the activation of disease resistance‐mediated cell death in planta. We explored the possibility that Pst DC3000 delivers AvrRpt2 protein to plant cells via the hrp (type III) secretion pathway. We now provide direct evidence that mature AvrRpt2 protein is secreted from Pst DC3000 and that secretion is hrp dependent. We also show that AvrRpt2 is N‐terminally processed when Arabidopsis thaliana plants are infected with Pst DC3000 expressing avrRpt2. Similar N‐terminal processing of AvrRpt2 occurred when avrRpt2 was stably expressed in A. thaliana. No cleavage of AvrRpt2 was detected in bacteria expressing avrRpt2 in culture or in the plant extracellular fluids. The N‐terminus of AvrRpt2 was not required for RPS2 recognition in planta. However, this region of AvrRpt2 was essential for Pst DC3000‐mediated elicitation of RPS2‐dependent cell death in A. thaliana leaves.
Journal
Molecular Microbiology – Wiley
Published: Jun 1, 1999