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References (12)
-
R. Schroff, C. Bucana, R. Klein, M. Farrell, A. Morgan (1984)
Detection of intracytoplasmic antigens by flow cytometry.Journal of immunological methods, 70 2
-
M. Labalette-Houache, G. Torpier, A. Capron, J. Dessaint (1991)
Improved permeabilization procedure for flow cytometric detection of internal antigens. Analysis of interleukin-2 production.Journal of immunological methods, 138 2
-
S. Kimura, M. Ikeda-Saito (1988)
Human myeloperoxidase and thyroid peroxidase, two enzymes with separate and distinct physiological functions, are evolutionarily related members of the same gene familyProteins: Structure, 3
-
G. Halldén, U. Andersson, J. Hed, S. Johansson (1989)
A new membrane permeabilization method for the detection of intracellular antigens by flow cytometry.Journal of immunological methods, 124 1
-
C. Caldwell, H. Taylor (1986)
A rapid, no-wash technic for immunophenotypic analysis by flow cytometry.American journal of clinical pathology, 86 5
-
IC Slaper-Cortenbach, LG Admiraal, JM Kerr, EF Leeuwen, A. Borne, PA Tetteroo (1988)
Flow-cytometric detection of terminal deoxynucleotidyl transferase and other intracellular antigens in combination with membrane antigens in acute lymphatic leukemiasBlood, 72
-
Martti Syrjälä, M. Tiirikainen, Sten-Erik Jansson, Tom Krusius (1993)
Flow cytometric analysis of terminal deoxynucleotidyl transferase. A simplified method.American journal of clinical pathology, 99 3
-
MI Tiirikainen, MT Syrjälä, S‐E Jansson, T Krusius (1992)
Flow cytometric analysis of P‐glycoprotein in normal and leukemic cells, 65
-
N. Kartner, D. Evernden-Porelle, G. Bradley, V. Ling (1985)
Detection of P-glycoprotein in multidrug-resistant cell lines by monoclonal antibodiesNature, 316
-
M. Tiirikainen, E. Elonen, M. Syrjälä, S. Jansson, T. Krusius (1994)
Flow cytometric analysis of glutathione-S-transferase-pi in acute leukemia.Leukemia, 8 6
-
M. Kastan, D. Slamon, C. Civin (1989)
Expression of protooncogene c-myb in normal human hematopoietic cellsBlood, 73
-
M. Osborn, E. Debus, K. Weber (1984)
Monoclonal antibodies specific for vimentin.European journal of cell biology, 34 1
- Publisher
- Wiley
- Copyright
- Copyright © 1995 Wiley Subscription Services, Inc., A Wiley Company
- ISSN
- 1552-4922
- eISSN
- 1552-4930
- DOI
- 10.1002/cyto.990200410
- pmid
- 7587722
- Publisher site
- See Article on Publisher Site
Abstract
When analyzing leukocyte cell surface antigens by flow cytometry, leukocytes are usually first labeled in whole blood and the red blood cells are finally lysed with lysing solutions. The erythrocytes are lysed, but the leukocytes are expected to remain intact. Six commercial red blood cell lysing methods were investigated for possible leukocyte permeabilization effect. The effectiveness of permeabilization was studied by propidium iodide staining, and the detectability of intracellular antigens was studied by using monoclonal antibodies toward two model antigens. Most of the lysing methods caused permeabilization of at least part of the leukocytes, but only one method, already found in our previous studies, was applicable for complete permeabilization of leukocytes and for detection of intracellular antigens alone or simultaneously with the cell surface antigens. © 1995 Wiley‐Liss, Inc.
Journal
Cytometry Part A – Wiley
Published: Jan 1, 1995
Keywords: ; ; ; ; ;