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Construction and validation of a mCherry protein vector for promoter analysis in Lactobacillus acidophilus

Construction and validation of a mCherry protein vector for promoter analysis in Lactobacillus... Lactobacilli are widespread in natural environments and are increasingly being investigated as potential health modulators. In this study, we have adapted the broad-host-range vector pNZ8048 to express the mCherry protein (pRCR) to expand the usage of the mCherry protein for analysis of gene expression in Lactobacillus. This vector is also able to replicate in Streptococcus pneumoniae and Escherichia coli. The usage of pRCR as a promoter probe was validated in Lactobacillus acidophilus by characterizing the regulation of lactacin B expression. The results show that the regulation is exerted at the transcriptional level, with lbaB gene expression being specifically induced by co-culture of the L. acidophilus bacteriocin producer and the S. thermophilus STY-31 inducer bacterium. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Industrial Microbiology Biotechnology Springer Journals

Construction and validation of a mCherry protein vector for promoter analysis in Lactobacillus acidophilus

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References (29)

Publisher
Springer Journals
Copyright
Copyright © 2014 by Society for Industrial Microbiology and Biotechnology
Subject
Life Sciences; Microbiology; Biochemistry, general; Inorganic Chemistry; Genetic Engineering; Biotechnology; Bioinformatics
ISSN
1367-5435
eISSN
1476-5535
DOI
10.1007/s10295-014-1567-4
pmid
25533634
Publisher site
See Article on Publisher Site

Abstract

Lactobacilli are widespread in natural environments and are increasingly being investigated as potential health modulators. In this study, we have adapted the broad-host-range vector pNZ8048 to express the mCherry protein (pRCR) to expand the usage of the mCherry protein for analysis of gene expression in Lactobacillus. This vector is also able to replicate in Streptococcus pneumoniae and Escherichia coli. The usage of pRCR as a promoter probe was validated in Lactobacillus acidophilus by characterizing the regulation of lactacin B expression. The results show that the regulation is exerted at the transcriptional level, with lbaB gene expression being specifically induced by co-culture of the L. acidophilus bacteriocin producer and the S. thermophilus STY-31 inducer bacterium.

Journal

Journal of Industrial Microbiology BiotechnologySpringer Journals

Published: Dec 23, 2014

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