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A simple system for pea transformation

A simple system for pea transformation The lateral cotyledonary meristems of germinating Pisum sativum cv. Puget seeds were used to develop a reproducible Agrobacterium tumefaciens -mediated transformation system. This procedure exhibits distinct advantages over those previously reported, in that it uses dry seed as starting material, and the highly regenerable cotyledonary meristems rapidly produce transgenic shoots without an intermediate callus phase. This transformation regime facilitates the rapid generation of phenotypically normal, self-fertile plants containing functional transgenes inherited in a Mendelian fashion. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell Reports Springer Journals

A simple system for pea transformation

Plant Cell Reports , Volume 16 (8) – May 1, 1997

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References (27)

Publisher
Springer Journals
Copyright
Copyright © 1997 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Cell Biology
ISSN
0721-7714
eISSN
1432-203X
DOI
10.1007/BF01142315
Publisher site
See Article on Publisher Site

Abstract

The lateral cotyledonary meristems of germinating Pisum sativum cv. Puget seeds were used to develop a reproducible Agrobacterium tumefaciens -mediated transformation system. This procedure exhibits distinct advantages over those previously reported, in that it uses dry seed as starting material, and the highly regenerable cotyledonary meristems rapidly produce transgenic shoots without an intermediate callus phase. This transformation regime facilitates the rapid generation of phenotypically normal, self-fertile plants containing functional transgenes inherited in a Mendelian fashion.

Journal

Plant Cell ReportsSpringer Journals

Published: May 1, 1997

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