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Diagnosis of human parvovirus infection by dot‐blot hybridization using cloned viral DNA

Diagnosis of human parvovirus infection by dot‐blot hybridization using cloned viral DNA The human parvovirus can be detected in serum by the immunological techniques of immune electron microscopy (IEM), counterimmunoelectrophoresis (CIE), and radioimmunoassay (RIA). A portion of the genome of this virus has been cloned in pAT153 and used as a 32P‐labelled probe in dot‐blot hybridization assays to detect parvovirus DNA in serum specimens. This test proved a highly sensitive means of detecting virus in microlitre volumes of serum, giving positive results for samples containing 0.5 pg viral DNA, equivalent to 104 virus particles. Unlike CIE and RIA the test is not affected by the presence of parvovirus‐specific antibody in serum specimens, and has permitted virus to be detected in specimens obtained up to 11 days after the onset of clinical symptoms of aplastic crisis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Medical Virology Wiley

Diagnosis of human parvovirus infection by dot‐blot hybridization using cloned viral DNA

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References (22)

Publisher
Wiley
Copyright
Copyright © 1985 Wiley‐Liss, Inc., A Wiley Company
ISSN
0146-6615
eISSN
1096-9071
DOI
10.1002/jmv.1890150209
Publisher site
See Article on Publisher Site

Abstract

The human parvovirus can be detected in serum by the immunological techniques of immune electron microscopy (IEM), counterimmunoelectrophoresis (CIE), and radioimmunoassay (RIA). A portion of the genome of this virus has been cloned in pAT153 and used as a 32P‐labelled probe in dot‐blot hybridization assays to detect parvovirus DNA in serum specimens. This test proved a highly sensitive means of detecting virus in microlitre volumes of serum, giving positive results for samples containing 0.5 pg viral DNA, equivalent to 104 virus particles. Unlike CIE and RIA the test is not affected by the presence of parvovirus‐specific antibody in serum specimens, and has permitted virus to be detected in specimens obtained up to 11 days after the onset of clinical symptoms of aplastic crisis.

Journal

Journal of Medical VirologyWiley

Published: Jun 1, 1985

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