Access the full text.
Sign up today, get DeepDyve free for 14 days.
S. Rastogi, B. Joshi, P. Dasgupta, M. Morris, K. Wright, S. Chellappan (2006)
Prohibitin Facilitates Cellular Senescence by Recruiting Specific Corepressors To Inhibit E2F Target GenesMolecular and Cellular Biology, 26
Arianne Theiss, R. Idell, S. Srinivasan, J. Klapproth, Dean Jones, D. Merlin, S. Sitaraman (2007)
Prohibitin protects against oxidative stress in intestinal epithelial cellsThe FASEB Journal, 21
Gregor Steglich, W. Neupert, T. Langer (1999)
Prohibitins Regulate Membrane Protein Degradation by the m-AAA Protease in MitochondriaMolecular and Cellular Biology, 19
Jen-Chih Chen, Cai-Zhong Jiang, M. Reid (2005)
Silencing a prohibitin alters plant development and senescence.The Plant journal : for cell and molecular biology, 44 1
T. Tatsuta, K. Model, T. Langer (2004)
Formation of membrane-bound ring complexes by prohibitins in mitochondria.Molecular biology of the cell, 16 1
A. Boveris, Enrique Cadenas, Andrés Stoppani (1976)
Role of ubiquinone in the mitochondrial generation of hydrogen peroxide.The Biochemical journal, 156 2
M. Sells, J. Boyd, J. Chernoff (1999)
p21-Activated Kinase 1 (Pak1) Regulates Cell Motility in Mammalian FibroblastsThe Journal of Cell Biology, 145
Alcorta Da, Y. Xiong, Dale Phelps, G. Hannon, D. Beach, Barrett Jc (1996)
Involvement of the cyclin-dependent kinase inhibitor p16 (INK4a) in replicative senescence of normal human fibroblasts.Proceedings of the National Academy of Sciences of the United States of America, 93 24
T. Minamino, I. Komuro (2007)
Vascular Cell Senescence: Contribution to AtherosclerosisCirculation Research, 100
Eva Gonzalez, Ruqin Kou, T. Michel (2006)
Rac1 Modulates Sphingosine 1-Phosphate-mediated Activation of Phosphoinositide 3-Kinase/Akt Signaling Pathways in Vascular Endothelial Cells*Journal of Biological Chemistry, 281
M. Quintero, S. Colombo, A. Godfrey, S. Moncada (2006)
Mitochondria as signaling organelles in the vascular endothelium.Proceedings of the National Academy of Sciences of the United States of America, 103 14
J. Passos, T. Zglinicki (2006)
Oxygen free radicals in cell senescence: Are they signal transducers?Free Radical Research, 40
Sheng Wang, Baohua Zhang, D. Faller (2002)
Prohibitin requires Brg‐1 and Brm for the repression of E2F and cell growthThe EMBO Journal, 21
A. Roskams, V. Friedman, C. Wood, L. Walker, G. Owens, D. Stewart, M. Altus, D. Danner, Xiao‐T. Liu, J. McClung (1993)
Cell cycle activity and expression of prohibitin mRNAJournal of Cellular Physiology, 157
Sung-suk Chae, J. Paik, H. Furneaux, T. Hla (2004)
Requirement for sphingosine 1-phosphate receptor-1 in tumor angiogenesis demonstrated by in vivo RNA interference.The Journal of clinical investigation, 114 8
M. Sanz, W. Tsang, Esther Willems, L. Grivell, B. Lemire, H. Spek, L. Nijtmans (2003)
The Mitochondrial Prohibitin Complex Is Essential for Embryonic Viability and Germline Function in Caenorhabditis elegans*Journal of Biological Chemistry, 278
P. Piper, G. Jones, D. Bringloe, N. Harris, M. MacLean, M. Mollapour (2002)
The shortened replicative life span of prohibitin mutants of yeast appears to be due to defective mitochondrial segregation in old mother cellsAging Cell, 1
Chang Ahn, Jeong Lee, A. Hwang, W. Kim, H. Pai (2006)
Prohibitin is involved in mitochondrial biogenesis in plants.The Plant journal : for cell and molecular biology, 46 4
P. Bernatchez, M. Winstead, E. Dennis, M. Sirois (2001)
VEGF stimulation of endothelial cell PAF synthesis is mediated by group V 14 kDa secretory phospholipase A2British Journal of Pharmacology, 134
C. Gourlay, K. Ayscough (2005)
The actin cytoskeleton: a key regulator of apoptosis and ageing?Nature Reviews Molecular Cell Biology, 6
F. Leeuwen, S. Delft, H. Kain, R. Kammen, John Collard (1999)
Rac regulates phosphorylation of the myosin-II heavy chain, actinomyosin disassembly and cell spreadingNature Cell Biology, 1
A. Boveris, B. Chance (1973)
The mitochondrial generation of hydrogen peroxide. General properties and effect of hyperbaric oxygen.The Biochemical journal, 134 3
D. Edwards, L. Sanders, G. Bokoch, G. Gill (1999)
Activation of LIM-kinase by Pak1 couples Rac/Cdc42 GTPase signalling to actin cytoskeletal dynamicsNature Cell Biology, 1
Gina Fusaro, P. Dasgupta, S. Rastogi, B. Joshi, S. Chellappan (2003)
Prohibitin Induces the Transcriptional Activity of p53 and Is Exported from the Nucleus upon Apoptotic Signaling*Journal of Biological Chemistry, 278
R. Oeckler, Elizabeth Arcuino, M. Ahmad, S. Olson, M. Wolin (2005)
Cytosolic NADH redox and thiol oxidation regulate pulmonary arterial force through ERK MAP kinase.American journal of physiology. Lung cellular and molecular physiology, 288 6
K. Connor, Sita Subbaram, Kevin Regan, K. Nelson, Joseph Mazurkiewicz, P. Bartholomew, Andrew Aplin, Y. Tai, J. Aguirre-Ghiso, Sonia Flores, J. Meléndez (2005)
Mitochondrial H2O2 Regulates the Angiogenic Phenotype via PTEN Oxidation*Journal of Biological Chemistry, 280
Goberdhan Dimri, X. Lee, G. Basile, M. Acosta, G. Scott, C. Roskelley, E. Medrano, M. Linskens, I. Rubelj, O. Pereira-smith (1995)
A biomarker that identifies senescent human cells in culture and in aging skin in vivo.Proceedings of the National Academy of Sciences of the United States of America, 92 20
S. Moncada, J. Erusalimsky (2002)
Does nitric oxide modulate mitochondrial energy generation and apoptosis?Nature Reviews Molecular Cell Biology, 3
Eva Gonzalez, Ruqin Kou, T. Michel (2006)
Rac 1 Modulates Sphingosine 1-Phosphate-mediated Activation of Phosphoinositide 3-Kinase / Akt Signaling Pathways in Vascular Endothelial Cells *
Hideyuki Miyauchi, T. Minamino, Kaoru Tateno, T. Kunieda, H. Toko, I. Komuro (2004)
Akt negatively regulates the in vitro lifespan of human endothelial cells via a p53/p21‐dependent pathwayThe EMBO Journal, 23
Sheng Wang, Gina Fusaro, J. Padmanabhan, S. Chellappan (2002)
Prohibitin co-localizes with Rb in the nucleus and recruits N-CoR and HDAC1 for transcriptional repressionOncogene, 21
M. Fukata, M. Nakagawa, K. Kaibuchi (2003)
Roles of Rho-family GTPases in cell polarisation and directional migration.Current opinion in cell biology, 15 5
A. Sewing, Bryony Wiseman, Alison Lloyd, H. Land (1997)
High-intensity Raf signal causes cell cycle arrest mediated by p21Cip1Molecular and Cellular Biology, 17
H. Cai (2005)
Hydrogen peroxide regulation of endothelial function: origins, mechanisms, and consequences.Cardiovascular research, 68 1
Haifeng Zhang, Yan Luo, Wei Zhang, Yun He, Shengchuan Dai, Rong Zhang, Yan Huang, P. Bernatchez, F. Giordano, G. Shadel, W. Sessa, W. Min (2007)
Endothelial‐specific expression of mitochondrial thioredoxin improves endothelial cell function and reduces atherosclerotic lesionsThe FASEB Journal, 21
J. Gutierrez, S. Ballinger, V. Darley-Usmar, A. Landar (2006)
Free radicals, mitochondria, and oxidized lipids: the emerging role in signal transduction in vascular cells.Circulation research, 99 9
S. Rastogi, B. Joshi, Gina Fusaro, S. Chellappan (2006)
Camptothecin Induces Nuclear Export of Prohibitin Preferentially in Transformed Cells through a CRM-1-dependent Mechanism*Journal of Biological Chemistry, 281
N. Soga, N. Namba, S. McAllister, L. Cornelius, S. Teitelbaum, S. Dowdy, J. Kawamura, K. Hruska (2001)
Rho family GTPases regulate VEGF-stimulated endothelial cell motility.Experimental cell research, 269 1
Guanhua Du, A. Mouithys-Mickalad, Francis Sluse (1998)
Generation of superoxide anion by mitochondria and impairment of their functions during anoxia and reoxygenation in vitro.Free radical biology & medicine, 25 9
D. Eveleth, J. Marsh (1986)
Sequence and expression of the Cc gene, a member of the dopa decarboxylase gene cluster of Drosophila: possible translational regulation.Nucleic acids research, 14 15
P. Lucchesi, Souad Belmadani, K. Matrougui (2005)
Hydrogen peroxide acts as both vasodilator and vasoconstrictor in the control of perfused mouse mesenteric resistance arteriesJournal of Hypertension, 23
Isabel Morrow, R. Parton (2005)
Flotillins and the PHB Domain Protein Family: Rafts, Worms and AnaestheticsTraffic, 6
E. Ikonen, K. Fiedler, R. Parton, K. Simons (1995)
Prohibitin, an antiproliferative protein, is localized to mitochondriaFEBS Letters, 358
K. Takeshige, S. Minakami (1979)
NADH- and NADPH-dependent formation of superoxide anions by bovine heart submitochondrial particles and NADH-ubiquinone reductase preparation.The Biochemical journal, 180 1
M. Schleicher, Fredrik Brundin, Steffen Gross, W. Müller-Esterl, S. Oess (2005)
Cell Cycle-Regulated Inactivation of Endothelial NO Synthase through NOSIP-Dependent Targeting to the CytoskeletonMolecular and Cellular Biology, 25
K. Burridge, K. Wennerberg (2004)
Rho and Rac Take Center StageCell, 116
W. Kiosses, R. Daniels, C. Otey, G. Bokoch, M. Schwartz (1999)
A Role for P21-Activated Kinase in Endothelial Cell MigrationThe Journal of Cell Biology, 147
D. Zorov, C. Filburn, L. Klotz, J. Zweier, S. Sollott (2000)
Reactive Oxygen Species (Ros-Induced) Ros Release: A New Phenomenon Accompanying Induction of the Mitochondrial Permeability Transition in Cardiac MyocytesJournal of Experimental Medicine, 192
(2000)
11015441J. Exp. Med., 192
P. Coates, P. Coates, R. Nenutil, A. McGregor, S. Picksley, D. Crouch, P. Hall, E. Wright, E. Wright (2001)
Mammalian prohibitin proteins respond to mitochondrial stress and decrease during cellular senescence.Experimental cell research, 265 2
N. Madamanchi, M. Runge (2007)
Mitochondrial Dysfunction in AtherosclerosisCirculation Research, 100
Philip Coates, Derek Jamieson, Katherine Smart, Alan Prescott, Peter Hall (1997)
The prohibitin family of mitochondrial proteins regulate replicative lifespanCurrent Biology, 7
Z. Yang, T. Zheng, A. Zhang, B. Altura, B. Altura (1998)
Mechanisms of hydrogen peroxide-induced contraction of rat aorta.European journal of pharmacology, 344 2-3
K. Rajalingam, C. Wunder, V. Brinkmann, Y. Churin, M. Hekman, C. Sievers, U. Rapp, T. Rudel (2005)
Prohibitin is required for Ras-induced Raf–MEK–ERK activation and epithelial cell migrationNature Cell Biology, 7
(1999)
A Role for p 21-Activated Kinase in Endothelial Cell Migration
Prohibitin 1 (PHB1) is a highly conserved protein that is mainly localized to the inner mitochondrial membrane and has been implicated in regulating mitochondrial function in yeast. Because mitochondria are emerging as an important regulator of vascular homeostasis, we examined PHB1 function in endothelial cells. PHB1 is highly expressed in the vascular system and knockdown of PHB1 in endothelial cells increases mitochondrial production of reactive oxygen species via inhibition of complex I, which results in cellular senescence. As a direct consequence, both Akt and Rac1 are hyperactivated, leading to cytoskeletal rearrangements and decreased endothelial cell motility, e.g., migration and tube formation. This is also reflected in an in vivo angiogenesis assay, where silencing of PHB1 blocks the formation of functional blood vessels. Collectively, our results provide evidence that PHB1 is important for mitochondrial function and prevents reactive oxygen species–induced senescence and thereby maintains the angiogenic capacity of endothelial cells. Footnotes Abbreviations used in this paper: BAEC, bovine aortic endothelial cell; EC, endothelial cell; HUVEC, human umbilical vein EC; PAK, p21-activated kinase; PECAM-1, platelet EC adhesion molecule 1; PEG, polyethylene glycol; PHB1, prohibitin-1; PI3-K, phosphatidyl inositol 3-kinase; ROS, reactive oxygen species; SMC, smooth muscle cell. Submitted: 11 June 2007 Accepted: 10 December 2007
The Journal of Cell Biology – Rockefeller University Press
Published: Jan 14, 2008
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.