Access the full text.
Sign up today, get DeepDyve free for 14 days.
Jun‐ichi Kato, S. Fujisaki, K. Nakajima, Y. Nishimura, Miyuki Sato, A. Nakano (1999)
The Escherichia coli Homologue of Yeast Rer2, a Key Enzyme of Dolichol Synthesis, Is Essential for Carrier Lipid Formation in Bacterial Cell Wall SynthesisJournal of Bacteriology, 181
Tim Zeppenfeld, C. Larisch, J. Lengeler, K. Jahreis (2000)
Glucose Transporter Mutants of Escherichia coli K-12 with Changes in Substrate Recognition of IICBGlc and Induction Behavior of theptsG GeneJournal of Bacteriology, 182
P. Cherepanov, W. Wackernagel (1995)
Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant.Gene, 158 1
Sang‐Hwal Yoon, Ju-Eun Kim, Sook‐Hee Lee, Hye-Min Park, M. Choi, Jae-Yean Kim, Si-Hyoung Lee, Y. Shin, J. Keasling, Seon-Won Kim (2007)
Engineering the lycopene synthetic pathway in E. coli by comparison of the carotenoid genes of Pantoea agglomerans and Pantoea ananatisApplied Microbiology and Biotechnology, 74
L. Song (2003)
Detection of farnesyl diphosphate accumulation in yeast ERG9 mutants.Analytical biochemistry, 317 2
Tomohiro Kainou, K. Okada, Kengo Suzuki, T. Nakagawa, H. Matsuda, M. Kawamukai (2001)
Dimer Formation of Octaprenyl-diphosphate Synthase (IspB) Is Essential for Chain Length Determination of Ubiquinone*The Journal of Biological Chemistry, 276
Kristala Jones, Seon-Won Kim, Jay Keasling (2000)
Low-copy plasmids can perform as well as or better than high-copy plasmids for metabolic engineering of bacteria.Metabolic engineering, 2 4
T. Kuzuyama (2002)
Mevalonate and Nonmevalonate Pathways for the Biosynthesis of Isoprene UnitsBioscience, Biotechnology, and Biochemistry, 66
D. Pitera, C. Paddon, J. Newman, J. Keasling (2007)
Balancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli.Metabolic engineering, 9 2
M. Rohmer, hamed Knani, P. Simonin, B. Sutter, Hermann Sahmt (1993)
Isoprenoid biosynthesis in bacteria: a novel pathway for the early steps leading to isopentenyl diphosphate.The Biochemical journal, 295 ( Pt 2)
P. Beyer, K. Kreuz, H. Kleinig (1985)
Separation of mevalonate phosphates and isopentenyl pyrophosphate by thin-layer chromatography and of short-chain prenyl phosphates by ion-pair chromatography on a high-performance liquid chromatography column.Methods in enzymology, 111
Pyung-Cheon Lee, Benjamin Mijts, C. Schmidt-Dannert (2004)
Investigation of factors influencing production of the monocyclic carotenoid torulene in metabolically engineered Escherichia coliApplied Microbiology and Biotechnology, 65
Luke Yuan, P. Rouvière, R. Larossa, Wonchul Suh (2006)
Chromosomal promoter replacement of the isoprenoid pathway for enhancing carotenoid production in E. coli.Metabolic engineering, 8 1
M. Chang, J. Keasling (2006)
Production of isoprenoid pharmaceuticals by engineered microbesNature Chemical Biology, 2
W. Farmer, J. Liao (2000)
Improving lycopene production in Escherichia coli by engineering metabolic controlNature Biotechnology, 18
E. Bruenger, H. Rilling (1988)
Determination of isopentenyl diphosphate and farnesyl diphosphate in tissue samples with a comment on secondary regulation of polyisoprenoid biosynthesis.Analytical biochemistry, 173 2
Amitabha Das, Sang‐Hwal Yoon, Sook‐Hee Lee, Jae-Yean Kim, D. Oh, Seon-Won Kim (2007)
An update on microbial carotenoid production: application of recent metabolic engineering toolsApplied Microbiology and Biotechnology, 77
S. Fujisaki, T. Nishino, H. Katsuki (1986)
Isoprenoid synthesis in Escherichia coli. Separation and partial purification of four enzymes involved in the synthesis.Journal of biochemistry, 99 5
K. Datsenko, B. Wanner (2000)
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.Proceedings of the National Academy of Sciences of the United States of America, 97 12
C. Chiang, P. Chen, Y. Chao (2008)
Replicon‐free and markerless methods for genomic insertion of DNAs in phage attachment sites and controlled expression of chromosomal genes in Escherichia coliBiotechnology and Bioengineering, 101
Yutaka Saisho, Atsushi Morimoto, Tsuneji Umeda (1997)
Determination of farnesyl pyrophosphate in dog and human plasma by high-performance liquid chromatography with fluorescence detection.Analytical biochemistry, 252 1
H. Tong, S. Holstein, R. Hohl (2005)
Simultaneous determination of farnesyl and geranylgeranyl pyrophosphate levels in cultured cells.Analytical biochemistry, 336 1
W. Hunter (2007)
The Non-mevalonate Pathway of Isoprenoid Precursor Biosynthesis*Journal of Biological Chemistry, 282
Benjamin Mijts, C. Schmidt-Dannert (2003)
Engineering of secondary metabolite pathways.Current opinion in biotechnology, 14 6
N. Misawa, M. Nakagawa, Kazuo Kobayashi, S. Yamano, Y. Izawa, Katsumi Nakamura, K. Harashima (1990)
Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coliJournal of Bacteriology, 172
R.Kennedy Keller (1996)
Squalene synthase inhibition alters metabolism of nonsterols in rat liver.Biochimica et biophysica acta, 1303 3
U. Theobald, W. Mailinger, Michael Baltes, M. Rizzi, M. Reuss (1997)
In vivo analysis of metabolic dynamics in Saccharomyces cerevisiae : I. Experimental observations.Biotechnology and bioengineering, 55 2
M. Rohmer, M. Seemann, S. Horbach, S. Bringer-Meyer, H. Sahm (1996)
Glyceraldehyde 3-Phosphate and Pyruvate as Precursors of Isoprenic Units in an Alternative Non-mevalonate Pathway for Terpenoid BiosynthesisJournal of the American Chemical Society, 118
T. Baba, J. Muth, C. Allen (1985)
Photoaffinity labeling of undecaprenyl pyrophosphate synthetase with a farnesyl pyrophosphate analogue.The Journal of biological chemistry, 260 19
Sang‐Hwal Yoon, H. Park, Ju-Eun Kim, Sook‐Hee Lee, M. Choi, Jae-Yean Kim, D. Oh, J. Keasling, Seon-Won Kim (2008)
Increased β‐Carotene Production in Recombinant Escherichia coli Harboring an Engineered Isoprenoid Precursor Pathway with Mevalonate AdditionBiotechnology Progress, 23
V. Martin, D. Pitera, Sydnor Withers, J. Newman, J. Keasling (2003)
Engineering a mevalonate pathway in Escherichia coli for production of terpenoidsNature Biotechnology, 21
C. Albermann, S. Ghanegaonkar, K. Lemuth, T. Vallon, M. Reuss, W. Armbruster, G. Sprenger (2008)
Biosynthesis of the Vitamin E Compound δ‐Tocotrienol in Recombinant Escherichia coli CellsChemBioChem, 9
A. Ruther, N. Misawa, P. Boger, G. Sandmann (1997)
Production of zeaxanthin in Escherichia coli transformed with different carotenogenic plasmidsApplied Microbiology and Biotechnology, 48
In biotechnology, the heterologous biosynthesis of isoprenoid compounds in Escherichia coli is a field of great interest and growth. In order to achieve higher isoprenoid yields in heterologous E. coli strains, it is necessary to quantify the pathway intermediates and adjust gene expression. In this study, we developed a precise and sensitive nonradioactive method for the simultaneous quantification of the isoprenoid precursors farnesyl diphosphate (FPP) and geranylgeranyl diphosphate (GGPP) in recombinant and wild-type E. coli cells. The method is based on the dephosphorylation of FPP and GGPP into the respective alcohols and involves their in situ extraction followed by separation and detection using gas chromatography–mass spectrometry. The integration of a geranylgeranyl diphosphate synthase gene into the E. coli chromosome leads to the accumulation of GGPP, generating quantities as high as those achieved with a multicopy expression vector.
Applied Microbiology and Biotechnology – Springer Journals
Published: Sep 24, 2008
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.