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- Publisher
- Springer Journals
- Copyright
- Copyright © 1990 by Springer-Verlag
- Subject
- Life Sciences; Plant Genetics and Genomics; Human Genetics; Microbial Genetics and Genomics; Animal Genetics and Genomics; Biochemistry, general
- ISSN
- 1617-4615
- eISSN
- 1432-1874
- DOI
- 10.1007/BF00261731
- Publisher site
- See Article on Publisher Site
Abstract
An integration vector for use in Methanococcus voltae was constructed, based on the Escherichia coli vector pUC18. It carries the structural gene for puromycin transacetylase from Streptomyces alboniger, which is flanked by expression signals of M. voltae structural genes and hisA gene sequences of this bacterium. Transformed M. voltae cells are puromycin resistant. Several types of integration of the vector into the chromosome were found. Only one case was due to nonhomologous recombination. The integrated sequences were stable under selective pressure but were slowly lost in some cases in the absence of the selective drug. The vector could be excised from M. voltae chromosomal DNA, recircularized and transformed back into E. coli.
Journal
Molecular Genetics and Genomics – Springer Journals
Published: Aug 8, 2004