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Identification of neuropeptide Y receptors in cultured astrocytes from neonatal rat brain

Identification of neuropeptide Y receptors in cultured astrocytes from neonatal rat brain Specific binding sites for neuropeptide Y could be demonstrated in primary cultures of astrocytes from neonatal rat brain. Neuropeptide Y binding was saturable, reversible, and temperature dependent as revealed by saturation studies and kinetic experiments. Scatchard analysis of equilibrium binding data indicated a single population of high‐affinity binding sites with respective KD and Bmax values of 0.43 nM and 6.9 fmol/2.7 × 105 cells. Physiological responses induced by neuropeptide Y could be detected in a distinct subpopulation of cultured astrocytes on the basis of two criteria: (1) electrophysiological responses and (2) single cell measurements of changes in (Ca2+)i. In that fraction of cells responding (20–70%, varying among cultures from different preparations), brief application of neuropeptide Y led to a membrane potential depolarization, lasting several minutes. When the membrane was clamped close to the resting membrane potential using the whole‐cell patch‐clamp technique, neuropeptide Y induced an inward current with a similar time course as the neuropeptide Y‐induced membrane depolarization. As detected by single cell microfluorimetric (fura‐2) measurements neuropeptide Y induced an increase of (Ca2+)i which was caused by the entry of extracellular Ca2+. Both the (Ca2+)i increase and the electrophysiological responses were unaffected by pretreatment of the astrocytes with pertussis toxin. © 1993 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neuroscience Research Wiley

Identification of neuropeptide Y receptors in cultured astrocytes from neonatal rat brain

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References (45)

Publisher
Wiley
Copyright
Copyright © 1993 Wiley‐Liss, Inc.
ISSN
0360-4012
eISSN
1097-4547
DOI
10.1002/jnr.490340207
pmid
8450563
Publisher site
See Article on Publisher Site

Abstract

Specific binding sites for neuropeptide Y could be demonstrated in primary cultures of astrocytes from neonatal rat brain. Neuropeptide Y binding was saturable, reversible, and temperature dependent as revealed by saturation studies and kinetic experiments. Scatchard analysis of equilibrium binding data indicated a single population of high‐affinity binding sites with respective KD and Bmax values of 0.43 nM and 6.9 fmol/2.7 × 105 cells. Physiological responses induced by neuropeptide Y could be detected in a distinct subpopulation of cultured astrocytes on the basis of two criteria: (1) electrophysiological responses and (2) single cell measurements of changes in (Ca2+)i. In that fraction of cells responding (20–70%, varying among cultures from different preparations), brief application of neuropeptide Y led to a membrane potential depolarization, lasting several minutes. When the membrane was clamped close to the resting membrane potential using the whole‐cell patch‐clamp technique, neuropeptide Y induced an inward current with a similar time course as the neuropeptide Y‐induced membrane depolarization. As detected by single cell microfluorimetric (fura‐2) measurements neuropeptide Y induced an increase of (Ca2+)i which was caused by the entry of extracellular Ca2+. Both the (Ca2+)i increase and the electrophysiological responses were unaffected by pretreatment of the astrocytes with pertussis toxin. © 1993 Wiley‐Liss, Inc.

Journal

Journal of Neuroscience ResearchWiley

Published: Feb 1, 1993

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