Access the full text.
Sign up today, get DeepDyve free for 14 days.
C. Tharaud, A. Ribet, C. Costes, C. Gaillardin (1992)
Secretion of human blood coagulation factor XIIIa by the yeast Yarrowia lipolytica.Gene, 121 1
K. Rane, K. Sims (1994)
Oxygen uptake and citric acid production by Candida lipolytica Y 1095Biotechnology and Bioengineering, 43
R. Buckholz (1993)
Yeast systems for the expression of heterologous gene products.Current opinion in biotechnology, 4 5
Merja Panttilä, L. André, M. Saloheimo, P. Lehtovaara, J. Knowles (1987)
Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevisiaeYeast, 3
V. Farkaš, M. Lišková, P. Biely (1985)
Novel media for detection of microbial producers of cellulase and xylanaseFems Microbiology Letters, 28
A. Silva, J. Benitez, C. Hollenberg (1991)
Endoglucanase A gene fusion vectors for monitoring protein secretion and glycosylation in yeast.Analytical biochemistry, 197 2
J. Sambrook, E. Fritsch, T. Maniatis (2001)
Molecular Cloning: A Laboratory Manual
R. Buckholz, M. Gleeson (1991)
Yeast Systems for the Commercial Production of Heterologous ProteinsBio/Technology, 9
S. Matoba, J. Fukayama, R Wing, D. Ogrydziak (1988)
Intracellular precursors and secretion of alkaline extracellular protease of Yarrowia lipolytica.Molecular and cellular biology, 8 11
C. Enderlin, D. Ogrydziak (1994)
Cloning, nucleotide sequence and functions of XPR6, which codes for a dibasic processing endoprotease from the yeast Yarrowia lipolyticaYeast, 10
Denis Lafontaine, David Tollervey (1996)
One-step PCR mediated strategy for the construction of conditionally expressed and epitope tagged yeast proteins.Nucleic acids research, 24 17
J. Owolabi, P. Béguin, D. Kilburn, Robert Miller, R. Warren (1988)
Expression in Escherichia coli of the Cellulomonas fimi Structural Gene for Endoglucanase BApplied and Environmental Microbiology, 54
M. Bailey, K. Nevalainen (1981)
Induction, isolation and testing of stable Trichoderma reesei mutants with improved production of solubilizing cellulaseEnzyme and Microbial Technology, 3
M. Niku‐Paavola, A. Lappalainen, T. Enari, M. Nummi (1985)
A new appraisal of the endoglucanases of the fungus Trichoderma reesei.The Biochemical journal, 231 1
Warren Gilkes, B. Henrissat, D. Kilburn, R. Miller, N. Gilkes, R. Warren (1991)
Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families.Microbiological reviews, 55 2
W. Wong, C. Curry, R. Parekh, S. Parekh, M. Wayman, R. Davies, D. Kilburn, N. Skipper (1988)
Wood Hydrolysis by Cellulomonas Fimi Endoglucanase and Exogiucanase Coexpressed as Secreted Enzymes in Saccharomyces CerevisiaeBio/Technology, 6
R. Horton, H. Hunt, S. Ho, J. Pullen, L. Pease (1989)
Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.Gene, 77 1
A. Harkki, A. Mäntylä, M. Penttilä, Susanna Muttilainen, Rolf Bühler, P. Suominen, Jonathan Knowles, Helena Nevalainen (1991)
Genetic engineering of Trichoderma to produce strains with novel cellulase profiles.Enzyme and microbial technology, 13 3
C. Chang, D. Ryu, C. Park, Jeong-Yoon Kim, D. Ogrydziak (1998)
Recombinant bioprocess optimization for heterologous protein production using two-stage, cyclic fed-batch cultureApplied Microbiology and Biotechnology, 49
Cheon-Seok Park, C. Chang, Jeong-Yoon Kim, D. Ogrydziak, D. Ryu (1997)
Expression, Secretion, and Processing of Rice α-Amylase in the Yeast Yarrowia lipolytica*The Journal of Biological Chemistry, 272
P. Sudbery (1996)
The expression of recombinant proteins in yeasts.Current opinion in biotechnology, 7 5
U. Laemmli (1970)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 227
D. Hanahan, D. Hanahan (1983)
Studies on transformation of Escherichia coli with plasmids.Journal of molecular biology, 166 4
J. Arsdell, S. Kwok, V. Schweickart, M. Ladner, D. Gelfand, M. Innis (1987)
Cloning, Characterization, and Expression in Saccharomyces Cerevisiae of Endoglucanase I from Trichoderma ReeseiBio/Technology, 5
J. Nicaud, P. Fournier, C. Bonnardiere, M. Chasles, C. Gaillardin (1991)
Use of ars18 based vectors to increase protein production in Yarrowia lipolytica.Journal of biotechnology, 19 2-3
P. Béguin, J. Aubert (1994)
The biological degradation of cellulose.FEMS microbiology reviews, 13 1
The endoglucanase I (EGI) from fungus Trichoderma reesei was cloned, expressed, and secreted from Yarrowia lipolytica using the XPR2 promoter. The signal sequence of EGI transferred from T. reesei was efficiently processed in the Y. lipolytica secretory pathway and directed the secretion of active EGI into the culture medium. However, the recombinant EGI produced from YLCSIn strain was hyperglycosylated and significantly larger than the native enzyme produced by the parent strain. The expression of EGI using XPR2 preproregion has caused secretion of modified proteins that still retained cellulase activity. This resulted from imprecise processing of the N-terminus of recombinant protein. While the batch culture produced 5 mg EGI/L from YLCSIn strain, the EGI yield was increased approx 20-fold when the fed-batch fermentation process strategy in combination with the high-cell density cultivation technique was employed. These results showed that the Y. lipolytica is a useful host organism for production of a large amount of large size heterologous proteins, especially when used in combination with high-cell density and fed-batch culture techniques.
Applied Biochemistry and Biotechnology – Springer Journals
Published: Apr 12, 2007
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.