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S. Jung, M. Oh (2000)
Iridovirus‐like infection associated with high mortalities of striped beakperch, Oplegnathus fasciatus (Temminck et Schlegel), in southern coastal areas of the Korean peninsulaJournal of Fish Diseases, 23
M. Miyata, K. Matsuno, S. Jung, Y. Danayadol, T. Miyazaki (1997)
Genetic similarity of iridoviruses from Japan and ThailandJournal of Fish Diseases, 20
F. Chua, M. Ng, K. Ng, J. Loo, J. Wee (1994)
Investigation of outbreaks of a novel disease, ‘Sleepy Grouper Disease’, affecting the brown‐spotted grouper, Epinephelus tauvina ForskalJournal of Fish Diseases, 17
J. Sambrook, E. Fritsch, T. Maniatis (2001)
Molecular Cloning: A Laboratory Manual
K. Nakajima, K. Inouye, M. Sorimachi (1998)
Viral Diseases in Cultured Marine Fish in JapanFish Pathology, 33
K. Nakajima, M. Sorimachi (1994)
Biological and physico-chemical properties of the iridovirus isolated from cultured red sea bream, Pagrus majorFish Pathology, 29
K. Inouye, K. Yamano, Y. Maeno, K. Nakajima, Manabu Matsuoka, Yuji Wada, M. Sorimachi (1992)
Iridovirus infection of cultured red sea bream, Pagrus majorFish Pathology, 27
L. Watson, J. Groff, R. Hedrick (1998)
Replication and pathogenesis of white sturgeon iridovirus (WSIV) in experimentally infected white sturgeon Acipenser transmontanus juveniles and sturgeon cell lines.Diseases of aquatic organisms, 32 3
S. Matsuoka, K. Inouye, K. Nakajima (1996)
Cultured Fish Species Affected by Red Sea Bream Iridoviral Disease from 1991 to 1995Fish Pathology, 31
S. Oshima, J. Hata, N. Hirasawa, T. Ohtaka, I. Hirono, T. Aoki, S. Yamashita (1998)
Rapid diagnosis of red sea bream iridovirus infection using the polymerase chain reaction.Diseases of aquatic organisms, 32 2
Y. Danayadol, S. Direkbusarakom, S. Boonyaratpalin, T. Miyazaki, M. Miyata (1997)
Iridovirus infection in brown-spotted grouper (Epinephelus malabaricus) cultured in Thailand
J. Kurita, K. Nakajima, I. Hirono, T. Aoki (1998)
Polymerase Chain Reaction (PCR) Amplification of DNA of Red Sea Bream Iridovirus (RSIV)Fish Pathology, 33
K. Nakajima, Y. Maeno, K. Yokoyama, Chie Kaji, S. Manabe (1998)
Antigen Analysis of Red Sea Bream Iridovirus and Comparison with other Fish IridovirusesFish Pathology, 33
H. Chou, C. Hsu, T. Peng (1998)
Isolation and Characterization of a Pathogenic Iridovirus from Cultured Grouper (Epinephelus sp.) in TaiwanFish Pathology, 33
J. Kasornchandra, R. Khongpradit (1996)
Isolation and preliminary characterization of a pathogenic iridovirus-like agent in nursing grouper, Epinephelus malabaricus
Since 1993, an epizootic viral disease has occurred in net‐cage cultured red sea bream, Pagrus major (Temminck & Schlegel), in Peng‐hu Island located on the south‐western coast of Taiwan. The diseased fish exhibited abnormal swimming and were lethargic, but few visible external signs were observed. The cumulative mortality because of the disease sometimes reached 50–90% over 2 months. Histopathogical studies of the affected fish showed enlarged basophilic cells in the gill, kidney, heart, liver and spleen. These necrotic cells were Feulgen‐positive and stained blue using Giemsa. Transmission electron microscopy revealed icosahedral virions in the cytoplasm of the necrotic cells. The viral particles consisted of a central nucleocapsid (75–80 nm) and envelope, and were 120–150 nm in diameter. These results suggest that the virus belongs to the Iridoviridae. Using polymerase chain reaction (PCR), approximately 570 bp fragments were produced from the viral DNA using as a template 1‐F and 1‐R primers derived from red seabream iridovirus (RSIV) from red sea bream in Japan. Similar results were also obtained using nested‐PCR with different primer sets (1‐F, 2‐R and 2‐F, 1‐R). Although the size and some features of epizootics of this virus differed from RSIV in Japan, it shows close genetic affinities with the latter and it is suggested that RSIV has been introduced to Taiwan.
Journal of Fish Diseases – Wiley
Published: Mar 1, 2003
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