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On the presence and distribution of olive viruses in Lebanon

On the presence and distribution of olive viruses in Lebanon A survey for viruses was carried out in 2003 in the main olive‐growing areas of Lebanon (South Lebanon, North Lebanon, Mount Lebanon and Bekaa). A total of 300 samples was collected in 31 different locations in 76 different commercial orchards and checked by RT‐PCR for the presence of Arabis mosaic virus (ArMV), Cherry leaf roll virus (CLRV), Strawberry latent ringspot virus (SLRV), Olive latent virus 1 (OLV‐1) and Olive leaf yellowing‐associated virus (OLYaV), using virus‐specific primers reported in the literature. About one third (31%) of the trees were infected. In particular, the closterovirus OLYaV was the most widespread, as it was detected in 23.7% of the samples, followed by the necrovirus OLV‐1 (8.3%), the two nepoviruses CLRV (2%) and ArMV (0.3%), and the sadwavirus SLRV (0.3%). A high variability was detected in the HSP70 gene of Lebanese and Italian OLYaV isolates, for at least nine different patterns were obtained when this genomic region was subjected to single‐strand conformation polymorphism (SSCP) analysis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png EPPO Bulletin Wiley

On the presence and distribution of olive viruses in Lebanon

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References (8)

Publisher
Wiley
Copyright
Copyright © 2005 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0250-8052
eISSN
1365-2338
DOI
10.1111/j.1365-2338.2005.00776.x
Publisher site
See Article on Publisher Site

Abstract

A survey for viruses was carried out in 2003 in the main olive‐growing areas of Lebanon (South Lebanon, North Lebanon, Mount Lebanon and Bekaa). A total of 300 samples was collected in 31 different locations in 76 different commercial orchards and checked by RT‐PCR for the presence of Arabis mosaic virus (ArMV), Cherry leaf roll virus (CLRV), Strawberry latent ringspot virus (SLRV), Olive latent virus 1 (OLV‐1) and Olive leaf yellowing‐associated virus (OLYaV), using virus‐specific primers reported in the literature. About one third (31%) of the trees were infected. In particular, the closterovirus OLYaV was the most widespread, as it was detected in 23.7% of the samples, followed by the necrovirus OLV‐1 (8.3%), the two nepoviruses CLRV (2%) and ArMV (0.3%), and the sadwavirus SLRV (0.3%). A high variability was detected in the HSP70 gene of Lebanese and Italian OLYaV isolates, for at least nine different patterns were obtained when this genomic region was subjected to single‐strand conformation polymorphism (SSCP) analysis.

Journal

EPPO BulletinWiley

Published: Apr 1, 2005

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