Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/rockefeller-university-press/hypotonicity-activates-a-native-chloride-current-in-xenopus-oocytes-rYYpvIRi2N
References
References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.
- Publisher
- Rockefeller University Press
- Copyright
- © 1994 Rockefeller University Press
- ISSN
- 0022-1295
- eISSN
- 1540-7748
- DOI
- 10.1085/jgp.103.2.153
- Publisher site
- See Article on Publisher Site
Abstract
Xenopus oocytes are frequently utilized for in vivo expression of cellular proteins, especially ion channel proteins. A thorough understanding of the endogenous conductances and their regulation is paramount for proper characterization of expressed channel proteins. Here we detail a novel chloride current (ICl.swell) responsive to hypotonicity in Xenopus oocytes using the two-electrode voltage clamp technique. Reducing the extracellular osmolarity by 50% elicited a calcium-independent chloride current having an anion conductivity sequence identical with swelling-induced chloride currents observed in epithelial cells. The hypotonicity-activated current was blocked by chloride channel blockers, trivalent lanthanides, and nucleotides. G-protein, cAMP-PKA, and arachidonic acid signaling cascades were not involved in ICl.swell activation. ICl.swell is distinct from both stretch-activated nonselective cation channels and the calcium-activated chloride current in oocytes and may play a critical role in volume regulation in Xenopus oocytes.
Journal
The Journal of General Physiology – Rockefeller University Press
Published: Feb 1, 1994