Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Two short‐chain dehydrogenase/reductases, NON‐YELLOW COLORING 1 and NYC1‐LIKE, are required for chlorophyll b and light‐harvesting complex II degradation during senescence in rice

Two short‐chain dehydrogenase/reductases, NON‐YELLOW COLORING 1 and NYC1‐LIKE, are required for... Yellowing, which is related to the degradation of chlorophyll and chlorophyll–protein complexes, is a notable phenomenon during leaf senescence. NON‐YELLOW COLORING1 (NYC1) in rice encodes a membrane‐localized short‐chain dehydrogenase/reductase (SDR) that is thought to represent a chlorophyll b reductase necessary for catalyzing the first step of chlorophyll b degradation. Analysis of the nyc1 mutant, which shows the stay‐green phenotype, revealed that chlorophyll b degradation is required for the degradation of light‐harvesting complex II and thylakoid grana in leaf senescence. Phylogenetic analysis further revealed the existence of NYC1‐LIKE (NOL) as the most closely related protein to NYC1. In the present paper, the nol mutant in rice was also found to show a stay‐green phenotype very similar to that of the nyc1 mutant, i.e. the degradation of chlorophyll b was severely inhibited and light‐harvesting complex II was selectively retained during senescence, resulting in the retention of thylakoid grana even at a late stage of senescence. The nyc1 nol double mutant did not show prominent enhancement of inhibition of chlorophyll degradation. NOL was localized on the stromal side of the thylakoid membrane despite the lack of a transmembrane domain. Immunoprecipitation analysis revealed that NOL and NYC1 interact physically in vitro. These observations suggest that NOL and NYC1 are co‐localized in the thylakoid membrane and act in the form of a complex as a chlorophyll b reductase in rice. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Plant Journal Wiley

Two short‐chain dehydrogenase/reductases, NON‐YELLOW COLORING 1 and NYC1‐LIKE, are required for chlorophyll b and light‐harvesting complex II degradation during senescence in rice

Download PDF
12 pages

Loading next page...
 
/lp/wiley/two-short-chain-dehydrogenase-reductases-non-yellow-coloring-1-and-rJvrSOGIfE

References (41)

Publisher
Wiley
Copyright
Copyright © 2009 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0960-7412
eISSN
1365-313X
DOI
10.1111/j.1365-313X.2008.03670.x
pmid
18778405
Publisher site
See Article on Publisher Site

Abstract

Yellowing, which is related to the degradation of chlorophyll and chlorophyll–protein complexes, is a notable phenomenon during leaf senescence. NON‐YELLOW COLORING1 (NYC1) in rice encodes a membrane‐localized short‐chain dehydrogenase/reductase (SDR) that is thought to represent a chlorophyll b reductase necessary for catalyzing the first step of chlorophyll b degradation. Analysis of the nyc1 mutant, which shows the stay‐green phenotype, revealed that chlorophyll b degradation is required for the degradation of light‐harvesting complex II and thylakoid grana in leaf senescence. Phylogenetic analysis further revealed the existence of NYC1‐LIKE (NOL) as the most closely related protein to NYC1. In the present paper, the nol mutant in rice was also found to show a stay‐green phenotype very similar to that of the nyc1 mutant, i.e. the degradation of chlorophyll b was severely inhibited and light‐harvesting complex II was selectively retained during senescence, resulting in the retention of thylakoid grana even at a late stage of senescence. The nyc1 nol double mutant did not show prominent enhancement of inhibition of chlorophyll degradation. NOL was localized on the stromal side of the thylakoid membrane despite the lack of a transmembrane domain. Immunoprecipitation analysis revealed that NOL and NYC1 interact physically in vitro. These observations suggest that NOL and NYC1 are co‐localized in the thylakoid membrane and act in the form of a complex as a chlorophyll b reductase in rice.

Journal

The Plant Journal – Wiley

Published: Jan 1, 2009

Keywords: ; ; ; ; ;

There are no references for this article.