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Gene expression in response to endoplasmic reticulum stress in Arabidopsis thaliana

Gene expression in response to endoplasmic reticulum stress in Arabidopsis thaliana Eukaryotic cells respond to the accumulation of unfolded proteins in the endoplasmic reticulum (ER). In this case, so‐called unfolded protein response (UPR) genes are induced. We determined the transcriptional expression of Arabidopsis thaliana UPR genes by fluid microarray analysis of tunicamycin‐treated plantlets. Two hundred and fifteen up‐regulated genes and 17 down‐regulated ones were identified. These genes were reanalyzed with functional DNA microarrays, using DNA fragments cloned through fluid microarray analysis. Finally, 36 up‐regulated and two down‐regulated genes were recognized as UPR genes. Among them, the up‐regulation of genes related to protein degradation (HRD1, SEL‐1L/HRD3 and DER1), regulation of translation (P58IPK), and apoptosis (BAX inhibitor‐1) was reconfirmed by real‐time reverse transcriptase‐PCR. The induction of SEL‐1L protein in an Arabidopsis membrane fraction on tunicamycin‐treatment was demonstrated. Phosphorylation of initiation factor‐2α, which was inhibited by P58IPK, was decreased in tunicamycin‐treated plantlets. However, regulatory changes in translation caused by ER stress were not detected in Arabidopsis. Plant cells appeared to have a strategy for overcoming ER stress through enhancement of protein folding activity, degradation of unfolded proteins, and regulation of apoptosis, but not regulation of translation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Febs Journal Wiley

Gene expression in response to endoplasmic reticulum stress in Arabidopsis thaliana

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References (80)

Publisher
Wiley
Copyright
Copyright © 2005 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1742-464X
eISSN
1742-4658
DOI
10.1111/j.1742-4658.2005.04770.x
pmid
15978049
Publisher site
See Article on Publisher Site

Abstract

Eukaryotic cells respond to the accumulation of unfolded proteins in the endoplasmic reticulum (ER). In this case, so‐called unfolded protein response (UPR) genes are induced. We determined the transcriptional expression of Arabidopsis thaliana UPR genes by fluid microarray analysis of tunicamycin‐treated plantlets. Two hundred and fifteen up‐regulated genes and 17 down‐regulated ones were identified. These genes were reanalyzed with functional DNA microarrays, using DNA fragments cloned through fluid microarray analysis. Finally, 36 up‐regulated and two down‐regulated genes were recognized as UPR genes. Among them, the up‐regulation of genes related to protein degradation (HRD1, SEL‐1L/HRD3 and DER1), regulation of translation (P58IPK), and apoptosis (BAX inhibitor‐1) was reconfirmed by real‐time reverse transcriptase‐PCR. The induction of SEL‐1L protein in an Arabidopsis membrane fraction on tunicamycin‐treatment was demonstrated. Phosphorylation of initiation factor‐2α, which was inhibited by P58IPK, was decreased in tunicamycin‐treated plantlets. However, regulatory changes in translation caused by ER stress were not detected in Arabidopsis. Plant cells appeared to have a strategy for overcoming ER stress through enhancement of protein folding activity, degradation of unfolded proteins, and regulation of apoptosis, but not regulation of translation.

Journal

Febs JournalWiley

Published: Jul 1, 2005

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