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EB viral antigens in burkitt tumor biopsies and early cultures

EB viral antigens in burkitt tumor biopsies and early cultures The emergence of cells producing EB viral antigens in cultures of Burkitt tumor biopsies was studied by means of immunofluorescence techniques. Definitely or questionably positive cells were seen in acetone‐fixed smears of 6% and 29% of the biopsies, respectively, which amounted to no more than a few cells among several hundred thousands. Yet in culture, specifically stainable cells often appeared within 3 to 10 days, occasionally in substantial numbers (up to 5%). Repeated biopsies from the same donors gave remarkably similar results. In time all cultures which developed into lines showed the presence of EBV. Arginine‐free medium failed to facilitate emergence of EBV antigen‐producing cells in young cultures or to increase their number, unless about 0.5% or more of the cells in the control cultures were productively infected. The implications of these findings are discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Cancer Wiley

EB viral antigens in burkitt tumor biopsies and early cultures

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References (19)

Publisher
Wiley
Copyright
Copyright © 1970 Wiley‐Liss, Inc., A Wiley Company
ISSN
0020-7136
eISSN
1097-0215
DOI
10.1002/ijc.2910060103
Publisher site
See Article on Publisher Site

Abstract

The emergence of cells producing EB viral antigens in cultures of Burkitt tumor biopsies was studied by means of immunofluorescence techniques. Definitely or questionably positive cells were seen in acetone‐fixed smears of 6% and 29% of the biopsies, respectively, which amounted to no more than a few cells among several hundred thousands. Yet in culture, specifically stainable cells often appeared within 3 to 10 days, occasionally in substantial numbers (up to 5%). Repeated biopsies from the same donors gave remarkably similar results. In time all cultures which developed into lines showed the presence of EBV. Arginine‐free medium failed to facilitate emergence of EBV antigen‐producing cells in young cultures or to increase their number, unless about 0.5% or more of the cells in the control cultures were productively infected. The implications of these findings are discussed.

Journal

International Journal of CancerWiley

Published: Jul 15, 1970

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