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Development of a model to demonstrate photosensitizer‐mediated viral inactivation in blood

Development of a model to demonstrate photosensitizer‐mediated viral inactivation in blood A model has been developed to demonstrate the use of photodynamic treatment (PDT) to eradicate viral contaminants from donated blood and blood products. Whole blood, spiked with vesicular stomatitis virus (VSV), was treated with the photosensitizer benzoporphyrin derivative‐monoacid ring A (BPD‐MA). After light activation of BPD‐MA, a neutral red dye uptake assay was carried out to determine virus inactivation. Various drug incubation times and light intensities were tested as well as red cell lysis and distribution of VSV in blood. At BPD‐MA concentrations between 2 and 4 μg per mL in whole blood, up to 107 VSV were inactivated. Several photosensitizers were also tested with this model to determine their relative efficacy in viral inactivation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Transfusion Wiley

Development of a model to demonstrate photosensitizer‐mediated viral inactivation in blood

Transfusion , Volume 30 (6) – Jul 8, 1990

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References (12)

Publisher
Wiley
Copyright
1990 AABB
ISSN
0041-1132
eISSN
1537-2995
DOI
10.1046/j.1537-2995.1990.30690333476.x
Publisher site
See Article on Publisher Site

Abstract

A model has been developed to demonstrate the use of photodynamic treatment (PDT) to eradicate viral contaminants from donated blood and blood products. Whole blood, spiked with vesicular stomatitis virus (VSV), was treated with the photosensitizer benzoporphyrin derivative‐monoacid ring A (BPD‐MA). After light activation of BPD‐MA, a neutral red dye uptake assay was carried out to determine virus inactivation. Various drug incubation times and light intensities were tested as well as red cell lysis and distribution of VSV in blood. At BPD‐MA concentrations between 2 and 4 μg per mL in whole blood, up to 107 VSV were inactivated. Several photosensitizers were also tested with this model to determine their relative efficacy in viral inactivation.

Journal

TransfusionWiley

Published: Jul 8, 1990

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