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(1975)
Tissue Culture ofthe Nervous System (SATO G., ed
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Protein measurement with the Folin phenol reagent.The Journal of biological chemistry, 193 1
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Astrocyte-specific protein and radial glia in the cerebral cortex of newborn ratNature, 252
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Identification and quantitation of antigens and antibodies by means of quantitative immunoelectrophoresis. A survey of methods.Journal of immunological methods, 1 2
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ANTIGEN—ANTIBODY CROSSED ELECTROPHORESIS OF WATER‐SOLUBLE RAT BRAIN ANTIGENSJournal of Neurochemistry, 18
E. Bock, O. Josrgensen, S. Morris (1974)
ANTIGEN‐ANTIBODY CROSSED ELECTROPHORESIS OF RAT BRAIN SYNAPTOSOMES AND SYNAPTIC VESICLES: CORRELATION TO WATER‐SOLUBLE ANTIGENS FROM RAT BRAINJournal of Neurochemistry, 22
H. Herschman, Barbara Grauling, M. Lerner (1973)
Nervous System-Specific Proteins in Cultured Neural Cells
Ole Jørgensen, E. Bock (1975)
Synaptic Plasma Mèmbrane Antigen D2 Measured in Human Cerebrospinal Fluid by Rocket‐Line Immunoelectrophoresis. Determination in Psychiatric and Neurological PatientsScandinavian Journal of Immunology, 4
B. Moore (1972)
Chemistry and biology of two proteins, S-100 and 14-3-2, specific to the nervous system.International review of neurobiology, 15
J. Schadé, H. Backer, E. Colon (1964)
Quantitative Analysis of Neuronal Parameters in the Maturing Cerebral CortexProgress in Brain Research, 4
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Complement Components in Normal Serum and Plasma Quantitated by ElectroimmunoassayScandinavian Journal of Immunology, 4
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Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence.Brain research, 43 2
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The chemical structure of synaptic membranes.Brain research, 62 2
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Brain specific synaptosomal membrane proteins demonstrated by crossed immunoelectrophoresisJournal of Neurochemistry, 23
H. Nielsen, Claus Koch (1975)
Genetic Control of the In Vitro Responses of Rat Blood LymphocytesScandinavian Journal of Immunology, 4
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Demonstration of Enolase Activity Connected to the Brain‐Specific Protein 14‐3‐2Scandinavian Journal of Immunology, 4
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Rat brain synaptic vesicles and synaptic plasma membranes compared by crossed immunoelectrophoresisFEBS Letters, 52
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IDENTIFICATION AND CHARACTERIZATION OF WATER SOLUBLE RAT BRAIN ANTIGENS I. BRAIN AND SPECIES SPECIFICITYJournal of Neurochemistry, 19
—The brain‐specific antigens 14·3·2, GFA, A5, F3, D1, D2, D3 and C1 were quantitated in a short‐term astroglial cell culture taken as a model of glial cells, and in synaptosomes, synaptosomal membranes and synaptic vesicles as neuronal material. Furthermore, the antigens were quantitated in newborn rat brain, as this served as the starting material for the cell culture. The membrane antigens C1, D1, D2 and D3 were absent from the cultured astroglia, indicating a neuronal origin for these antigens. C1 was enriched 3‐fold in synaptosomes and synaptosomal membranes and more than 10‐fold in synaptic vesicles indicating that this antigen might be a marker protein for nerve endings. The name Synaptin is introduced for this antigen. Conversely, the data on the antigens D1, D2 and D3 indicated that these antigens were not restricted to the synaptosomes although they were of neuronal origin. Trace amounts of the cathodal part of the heterogeneous cytoplasmic antigen 14·3·2 were present in the cell culture, possibly originating from a few contaminating neurons. The cytoplasmic antigens A5 and F3 were found both in the astroglial culture and in the synaptosomal fraction. F3, however, was found in low concentration in the synaptosomes and 3‐fold enriched in newborn rat brain compared to rat brain from 35‐day‐old rats or to 21‐day‐old brain cell cultures. It was therefore regarded as a brain specific fetal antigen. The antigen GFA was highly enriched in the astroglial culture compared to whole brain and only trace amounts were found in the synaptosomal fraction supporting the astroglial origin of this antigen.
Journal of Neurochemistry – Wiley
Published: Dec 1, 1975
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