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- Publisher
- Wiley
- Copyright
- Copyright © 1993 Wiley Subscription Services, Inc., A Wiley Company
- ISSN
- 0962-1083
- eISSN
- 1365-294X
- DOI
- 10.1111/j.1365-294X.1993.tb00006.x
- Publisher site
- See Article on Publisher Site
Abstract
This article reviews four ‘DNA screening techniques’, namely heteroduplex analysis, single‐strand conformational polymorphism (SSCP), denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) as tools for the study of allelic variation in natural populations. The resolving power, advantages, and limitations of each technique are discussed and compared. We also provide some criteria for choosing among techniques and illustrate some practical issues with examples taken primarily from our own laboratory experience.
Journal
Molecular Ecology – Wiley
Published: Apr 1, 1993
Keywords: ; ; ; ; ;