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C. Clemmesen (1988)
A RNA and DNA fluorescence technique to evaluate the nutritional condition of individual marine fish larvae, 32
L. Buckley (1979)
Relationships Between RNA–DNA Ratio, Prey Density, and Growth Rate in Atlantic Cod (Gadus morhua) LarvaeWsq: Women's Studies Quarterly, 36
Institut fur Hydrobiologie und Fischereiwissenschaft an der Universitat Hamburg, Olbersweg 24,2000 Hamburg 50, F.R.G. Key words: RNAiDNA ratio; fluorescence technique; starvation; laboratory-reared herring larvae; wild herring larvae. I t has been shown that the ratio of ribonucleic acid (RNA) to deoxyribonucleic acid (DNA) is a useful indicator of nutritional condition. Previous studies measuring RNA/DNA ratios in larval fish used a method which required a minimum of 800 pg dry weight per sample (Buckley, 1979), so pooled samples had to be analysed and estimates of individual variability in the RNA/DNA ratio were impossible. But such estimates might be essential for detecting starving individuals, in determining whether starvation plays a major role in the recruitment process. Therefore, a more sensitive fluorometric method for RNA/DNA quantification in individual fish (Clemmesen, 1988) was applied to fed and starving laboratory-reared herring, Clupea harengus L., larvae and to field-caught herring larvae. Fertilized eggs from spring-spawning herring were incubated and the larvae were fed on Blac.hionusplicatilis and Artemia nauplii or deprived of food for periods of 2-6 days. For the assessment of possible starvation in the wild, special tows with a ' Helgolander larvae net ' were made during a herring larvae survey by R.V.
Journal of Fish Biology – Wiley
Published: Dec 1, 1989
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