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RNA/DNA ratios of laboratory‐reared and wild herring larvae determined with a highly sensitive fluorescence method

RNA/DNA ratios of laboratory‐reared and wild herring larvae determined with a highly sensitive... Institut fur Hydrobiologie und Fischereiwissenschaft an der Universitat Hamburg, Olbersweg 24,2000 Hamburg 50, F.R.G. Key words: RNAiDNA ratio; fluorescence technique; starvation; laboratory-reared herring larvae; wild herring larvae. I t has been shown that the ratio of ribonucleic acid (RNA) to deoxyribonucleic acid (DNA) is a useful indicator of nutritional condition. Previous studies measuring RNA/DNA ratios in larval fish used a method which required a minimum of 800 pg dry weight per sample (Buckley, 1979), so pooled samples had to be analysed and estimates of individual variability in the RNA/DNA ratio were impossible. But such estimates might be essential for detecting starving individuals, in determining whether starvation plays a major role in the recruitment process. Therefore, a more sensitive fluorometric method for RNA/DNA quantification in individual fish (Clemmesen, 1988) was applied to fed and starving laboratory-reared herring, Clupea harengus L., larvae and to field-caught herring larvae. Fertilized eggs from spring-spawning herring were incubated and the larvae were fed on Blac.hionusplicatilis and Artemia nauplii or deprived of food for periods of 2-6 days. For the assessment of possible starvation in the wild, special tows with a ' Helgolander larvae net ' were made during a herring larvae survey by R.V. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Fish Biology Wiley

RNA/DNA ratios of laboratory‐reared and wild herring larvae determined with a highly sensitive fluorescence method

Journal of Fish Biology , Volume 35 – Dec 1, 1989

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References (2)

Publisher
Wiley
Copyright
Copyright © 1989 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-1112
eISSN
1095-8649
DOI
10.1111/j.1095-8649.1989.tb03078.x
Publisher site
See Article on Publisher Site

Abstract

Institut fur Hydrobiologie und Fischereiwissenschaft an der Universitat Hamburg, Olbersweg 24,2000 Hamburg 50, F.R.G. Key words: RNAiDNA ratio; fluorescence technique; starvation; laboratory-reared herring larvae; wild herring larvae. I t has been shown that the ratio of ribonucleic acid (RNA) to deoxyribonucleic acid (DNA) is a useful indicator of nutritional condition. Previous studies measuring RNA/DNA ratios in larval fish used a method which required a minimum of 800 pg dry weight per sample (Buckley, 1979), so pooled samples had to be analysed and estimates of individual variability in the RNA/DNA ratio were impossible. But such estimates might be essential for detecting starving individuals, in determining whether starvation plays a major role in the recruitment process. Therefore, a more sensitive fluorometric method for RNA/DNA quantification in individual fish (Clemmesen, 1988) was applied to fed and starving laboratory-reared herring, Clupea harengus L., larvae and to field-caught herring larvae. Fertilized eggs from spring-spawning herring were incubated and the larvae were fed on Blac.hionusplicatilis and Artemia nauplii or deprived of food for periods of 2-6 days. For the assessment of possible starvation in the wild, special tows with a ' Helgolander larvae net ' were made during a herring larvae survey by R.V.

Journal

Journal of Fish BiologyWiley

Published: Dec 1, 1989

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