Access the full text.
Sign up today, get DeepDyve free for 14 days.
C. Verweij, C. Guidos, G. Crabtree (1990)
Cell type specificity and activation requirements for NFAT-1 (nuclear factor of activated T-cells) transcriptional activity determined by a new method using transgenic mice to assay transcriptional activity of an individual nuclear factor.The Journal of biological chemistry, 265 26
Michael Rosen, Robert Standaert, Andrzej Galat, Masashi Nakatsuka, Stuart Schreiber (1990)
Inhibition of FKBP rotamase activity by immunosuppressant FK506: twisted amide surrogate.Science, 248 4957
D. Durand, '. JENG-PYNG, Shaw, E. Robert, Replogle, Rama Belagaje, R. Gerald, '. Crabtree (1988)
Characterization of antigen receptor response elements within the interleukin-2 enhancerMolecular and Cellular Biology, 8
B. Willumsen, Annette Christensen, N. Hubbert, A. Papageorge, D. Lowy (1984)
The p21 ras C-terminus is required for transformation and membrane associationNature, 310
P. Belshaw, Steffan Ho, Gerald Crabtree, Stuart Schreiber (1996)
Controlling protein association and subcellular localization with a synthetic ligand that induces heterodimerization of proteins.Proceedings of the National Academy of Sciences of the United States of America, 93 10
R. Wange, Ramón Guitián, N. Isakov, J. Watts, R. Aebersold, L. Samelson (1995)
Activating and Inhibitory Mutations in Adjacent Tyrosines in the Kinase Domain of ZAP-70 (*)The Journal of Biological Chemistry, 270
J. Berger, J. Hauber, R. Hauber, R. Geiger, B. Cullen (1988)
Secreted placental alkaline phosphatase: a powerful new quantitative indicator of gene expression in eukaryotic cells.Gene, 66 1
D. Mochly‐Rosen (1995)
Localization of protein kinases by anchoring proteins: a theme in signal transduction.Science, 268 5208
Richard Bram, Deborah Hung, Patrick Martin, Stuart Schreiber, Gerald Crabtree (1993)
Identification of the immunophilins capable of mediating inhibition of signal transduction by cyclosporin A and FK506: roles of calcineurin binding and cellular locationMolecular and Cellular Biology, 13
(1988)
The first T-cells) transcriptional activity determined by a new method
J. Buss, P. Solski, J. Schaeffer, M. Macdonald, C. Der (1989)
Activation of the cellular proto-oncogene product p21Ras by addition of a myristylation signal.Science, 243 4898
(1992)
in RIPA buffer; Extracts were analyzed by SDS-PAGE and electrotrans-(1995) Caveolin cycles between plasma membrane caveolae and the ferred to nitrocellulose
R. Perlmutter, S. Levin, M. Appleby, Steven Anderson, José Alberola-Ila (1993)
Regulation of lymphocyte function by protein phosphorylation.Annual review of immunology, 11
Wei Li, C. Whaley, A. Mondino, D. Mueller (1996)
Blocked Signal Transduction to the ERK and JNK Protein Kinases in Anergic CD4+ T CellsScience, 271
Andrew Chan, M. Dalton, Robin Johnson, Guang-hui Kong, Tian Wang, Rich Thoma, Tomohiro Kurosaki (1995)
Activation of ZAP‐70 kinase activity by phosphorylation of tyrosine 493 is required for lymphocyte antigen receptor function.The EMBO Journal, 14
(1996)
Three-part inventions: peroxidase-conjugated rabbit anti-mouse IgG (Zymed)
Patricia Conrad, Eric Smart, Yun-shu Ying, R. Anderson, G. Bloom (1995)
Caveolin cycles between plasma membrane caveolae and the Golgi complex by microtubule-dependent and microtubule-independent stepsThe Journal of Cell Biology, 131
N. Clipstone, G. Crabtree (1992)
Identification of calcineurin as a key signalling enzyme in T-lymphocyte activationNature, 357
J. Kaplan, G. Mardon, J. Bishop, H. Varmus (1988)
The first seven amino acids encoded by the v-src oncogene act as a myristylation signal: lysine 7 is a critical determinantMolecular and Cellular Biology, 8
M. Reth (1989)
Antigen receptor tail clueNature, 338
B. Sefton, I. Trowbridge, Jonathan Cooper, E. Scolnick (1982)
The transforming proteins of Rous sarcoma virus, Harvey sarcoma virus and Abelson virus contain tightly bound lipidCell, 31
(1994)
Signal transduction by lymphocyte S.L. (1991) Calcineurin is a common target of cyclophilin-cyclosporin antigen receptors
M. Iwashima, B. Irving, N. Oers, A. Chan, A. Chan, Arthur Weiss (1994)
Sequential interactions of the TCR with two distinct cytoplasmic tyrosine kinases.Science, 263 5150
(1993)
AP-1-SEAP have been described elsewhere
Gerald Crabtree, Stuart Schreiber (1996)
Three-part inventions: intracellular signaling and induced proximity.Trends in biochemical sciences, 21 11
(1991)
Structure of human cyclophilin and its thymocytes and lymph node T cells
(1988)
Structure of the phosphatase was measured 18 h after stimulation, as described previously FKBP12–rapamycin complex interacting with the binding domain of
Andrew Chan, T. Kadlecek, M. Elder, A. Filipovich, W. Kuo, M. Iwashima, T. Parslow, A. Weiss (1994)
ZAP-70 deficiency in an autosomal recessive form of severe combined immunodeficiency.Science, 264 5165
L. Samelson, J. Harford, R. Schwartz, R. Klausner (1985)
A 20-kDa protein associated with the murine T-cell antigen receptor is phosphorylated in response to activation by antigen or concanavalin A.Proceedings of the National Academy of Sciences of the United States of America, 82 7
D. Spencer, I. Graef, D. Austin, S. Schreiber, G. Crabtree (1995)
A general strategy for producing conditional alleles of Src-like tyrosine kinases.Proceedings of the National Academy of Sciences of the United States of America, 92 21
J. Kallen, C. Spitzfaden, M. Zurini, G. Wider, H. Widmer, K. Wüthrich, M. Walkinshaw (1991)
Structure of human cyclophilin and its binding site for cyclosporin A determined by X-ray crystallography and NMR spectroscopyNature, 353
eukaryotic expression construct Lck dn . The constructs SF1Lck
J. Northrop, S. Ho, Lei Chen, D. Thomas, L. Timmerman, G. Nolan, A. Admon, G. Crabtree (1994)
NF-AT components define a family of transcription factors targeted in T-cell activationNature, 369
(1984)
Antigen-reactive cloned helper T Madrenas,J., Wange,R.L
TAg Jurkat cells were transfected by electroporation with 1 µg MF3E Biol
M. Hatada, Xiaode Lu, E. Laird, Jeremy Green, J. Morgenstern, M. Lou, C. Marr, T. Phillips, M. Ram, K. Theriault, M. Zoller, J. Karas (1995)
Molecular basis for interaction of the protein tyrosine kinase ZAP-70 with the T-cell receptorNature, 377
Joaquín Madrenas, R. Wange, Jennifer Wang, Noah Isakov, L. Samelson, Ronald Germain (1995)
Zeta phosphorylation without ZAP-70 activation induced by TCR antagonists or partial agonistsScience, 267
(1992)
The 70 kDa protein-tyrosine kinase that associates with the TCR zeta NF-ATc1 eukaryotic expression construct pSH160c (kindly provided by chain
A. Weiss, D. Littman (1994)
Signal transduction by lymphocyte antigen receptorsCell, 76
D. Wilde, F. Fitch (1984)
Antigen-reactive cloned helper T cells. I. Unresponsiveness to antigenic restimulation develops after stimulation of cloned helper T cells.Journal of immunology, 132 4
(1988)
Science
G. Duyne, R. Standaert, P. Karplus, S. Schreiber, J. Clardy (1993)
Atomic structures of the human immunophilin FKBP-12 complexes with FK506 and rapamycin.Journal of molecular biology, 229 1
M. Resh (1994)
Myristylation and palmitylation of Src family members: The fats of the matterCell, 76
W. Flanagan, B. Corthésy, R. Bram, G. Crabtree (1991)
Nuclear association of a T-cell transcription factor blocked by FK-506 and cyclosporin ANature, 352
S. Ho, S. Biggar, David Spencer, S. Schreiber, G. Crabtree (1996)
Dimeric ligands define a role for transcriptional activation domains in reinitiationNature, 382
Andrew Chan, B. Irving, J. Fraser, Arthur Weiss (1991)
The zeta chain is associated with a tyrosine kinase and upon T-cell antigen receptor stimulation associates with ZAP-70, a 70-kDa tyrosine phosphoprotein.Proceedings of the National Academy of Sciences of the United States of America, 88
(1990)
Cell type specificityand NMR spectroscopy
(1996)
Widmer,H. transduction to the ERK and JNK protein kinases in anergic CD4ϩ and Wüthrich,K. (1991) The NMR structure of cyclosporin A bound T cells
Andrew Chan, D. Desai, Arthur Weiss (1994)
The role of protein tyrosine kinases and protein tyrosine phosphatases in T cell antigen receptor signal transduction.Annual review of immunology, 12
T. Pawson, G. Gish (1992)
SH2 and SH3 domains: From structure to functionCell, 71
C. Weber, G. Wider, B. Freyberg, R. Traber, W. Braun, H. Widmer, K. Wüthrich (1991)
The NMR structure of cyclosporin A bound to cyclophilin in aqueous solution.Biochemistry, 30 26
C. Koch, D. Anderson, M. Moran, C. Ellis, T. Pawson (1991)
SH2 and SH3 domains: elements that control interactions of cytoplasmic signaling proteins.Science, 252 5006
L. Holsinger, D. Spencer, D. Austin, S. Schreiber, G. Crabtree (1995)
Signal transduction in T lymphocytes using a conditional allele of Sos.Proceedings of the National Academy of Sciences of the United States of America, 92 21
W. Kolanus, C. Romeo, B. Seed (1993)
T cell activation by clustered tyrosine kinasesCell, 74
N. Oers, Nigel Killeen, Arthur Welss (1994)
ZAP-70 is constitutively associated with tyrosine-phosphorylated TCR zeta in murine thymocytes and lymph node T cells.Immunity, 1 8
S. Dalton, R. Treisman (1992)
Characterization of SAP-1, a protein recruited by serum response factor to the c-fos serum response elementCell, 68
NA Clipstone, GR Crabtree (1992)
Identification of calcineurin as a key signalling enzyme in T cell activationNature, 357
R. Wange, A. Kong, L. Samelson (1992)
A tyrosine-phosphorylated 70-kDa protein binds a photoaffinity analogue of ATP and associates with both the zeta chain and CD3 components of the activated T cell antigen receptor.The Journal of biological chemistry, 267 17
J. Schnitzer, P. Oh, D. Mcintosh (1996)
Role of GTP Hydrolysis in Fission of Caveolae Directly from Plasma MembranesScience, 274
A. Chan, M. Iwashima, C. Turck, A. Weiss (1992)
ZAP-70: A 70 kd protein-tyrosine kinase that associates with the TCR ζ chainCell, 71
M. Elder, D. Lin, J. Clever, Andrew Chan, T. Hope, A. Weiss, T. Parslow (1994)
Human severe combined immunodeficiency due to a defect in ZAP-70, a T cell tyrosine kinase.Science, 264 5165
E. Arpaia, M. Shahar, H. Dadi, A. Cohen, Chaim Rolfman (1994)
Defective T cell receptor signaling and CD8+ thymic selection in humans lacking Zap-70 kinaseCell, 76
Y. Takebe, M. Seiki, J. Fujisawa, P. Hoy, K. Yokota, K. Arai, Mitsuaki Yoshida, N. Arai (1988)
SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeatMolecular and Cellular Biology, 8
Jun Liu, J. Farmer, W. Lane, J. Friedman, I. Weissman, S. Schreiber (1991)
Calcineurin is a common target of cyclophilin-cyclosporin A and FKBP-FK506 complexesCell, 66
M. Izquierdo, D. Cantrell (1992)
T-cell activation.Trends in cell biology, 2 9
C. Goodnow, J. Crosbie, S. Adelstein, T. Lavoie, S. Smith‐Gill, R. Brink, H. Pritchard-Briscoe, J. Wotherspoon, R. Loblay, Kathy Raphael, R. Trent, A. Basten (1988)
Altered immunoglobulin expression and functional silencing of self-reactive B lymphocytes in transgenic miceNature, 334
(1991)
The zeta chain et al . , 1988 ) . A Bgl II – Pfl MI fragment of SF 1 ∆ KLck ( K 273 R ) ( Spencer is associated with a tyrosine kinase and upon T - cell antigen receptor et al . ,
David Straus, Arthur Weiss (1993)
The CD3 chains of the T cell antigen receptor associate with the ZAP-70 tyrosine kinase and are tyrosine phosphorylated after receptor stimulationThe Journal of Experimental Medicine, 178
I. Negishi, N. Motoyama, K. Nakayama, Kei-ichi Nakayama, S. Senju, S. Hatakeyama, Qing Zhang, A. Chan, D. Loh (1995)
Essential role for ZAP-70 in both positive and negative selection of thymocytesNature, 376
R. Fotedar, A. Fotedar (1995)
Cell cycle control of DNA replication.Progress in cell cycle research, 1
DM Spencer, TJ Wandless, SL Schreiber, GR Crabtree (1993)
Controlling signal transduction with synthetic ligandsScience, 262
J. Sloan-Lancaster, A. Shaw, J. Rothbard, P. Allen (1994)
Partial T cell signaling: Altered phospho-ζ and lack of zap70 recruitment in APL-induced T cell anergyCell, 79
M. Faux, John Scott (1996)
Molecular Glue: Kinase Anchoring and Scaffold ProteinsCell, 85
S. Levin, S. Anderson, K. Forbush, R. Perlmutter (1993)
A dominant‐negative transgene defines a role for p56lck in thymopoiesis.The EMBO Journal, 12
F. Cross, E. Garber, D. Pellman, H. Hanafusa (1984)
A short sequence in the p60src N terminus is required for p60src myristylation and membrane association and for cell transformationMolecular and Cellular Biology, 4
P. Fields, T. Gajewski, F. Fitch (1996)
Blocked Ras Activation in Anergic CD4+ T CellsScience, 271
L. Gauen, Y. Zhu, F. Letourneur, Q. Hu, J. Bolen, L Matis, R. Klausner, A. Shaw (1994)
Interactions of p59fyn and ZAP-70 with T-cell receptor activation motifs: defining the nature of a signalling motifMolecular and Cellular Biology, 14
J Berger, J Hauber, R Hauber, R Geiger, BR Cullen (1988)
Secreted placental alkaline phosphatase: a powerful new quantitative indicator of gene expressionGene, 66
Jungwon Choi, Jie Chen, S. Schreiber, J. Clardy (1996)
Structure of the FKBP12-Rapamycin Complex Interacting with Binding Domain of Human FRAPScience, 273
Signaling by the antigen receptor of T lymphocytes initiates different developmental transitions, each of which require the tyrosine kinase ZAP70. Previous studies with agonist and antagonist peptides have indicated that ZAP70 might respond differently to different structures of the TCR–CD3 complex induced by bound peptides. The roles of membrane proximity and orientation in activation of ZAP70 signaling were explored using synthetic ligands and their binding proteins designed to produce different architectures of membrane‐bound complexes composed of ZAP70 fusion proteins. Transient membrane recruitment of physiological levels of ZAP70 with the membrane‐permeable synthetic ligand FK1012A leads to rapid phosphorylation of ZAP70 and activation of the ras/MAPK and Ca2+/calcineurin signaling pathways. ZAP70 SH2 domains are not required for signaling when the kinase is artifically recruited to the membrane, indicating that the SH2 domains function solely in recruitment and not in kinase activation. Using additional synthetic ligands and their binding proteins that recruit ZAP70 equally well but orient it at the cell membrane in different ways, we define a requirement for a specific presentation of ZAP70 to its downstream targets. These results provide a mechanism by which ZAP70, bound to the phosphorylated receptor, could discriminate between conformational changes induced by the binding of different MHC–peptide complexes to the antigen receptor and introduce an approach to exploring the role of spatial orientation of signaling complexes in living cells.
The EMBO Journal – Wiley
Published: Mar 15, 1998
Keywords: ; ; ; ;
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.