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S. Elmer, S. Rogers (1990)
Selection for wild type size derivatives of tomato golden mosaic virus during systemic infection.Nucleic acids research, 18 8
M. Huisman, H. Linthorst, J. Bol, Ben Cornelissen (1988)
The complete nucleotide sequence of potato virus X and its homologies at the amino acid level with various plus-stranded RNA viruses.The Journal of general virology, 69 ( Pt 8)
J. Stanley, R. Townsend (1986)
Infectious mutants of cassava latent virus generated in vivo from intact recombinant DNA clones containing single copies of the genome.Nucleic acids research, 14 15
D. Baulcombe, R. Flavell, R. Boulton, G. Jellis (1984)
The sensitivity and specificity of a rapid nucleic acid hybridization method for the detection of potato virus X in crude sap samples.Plant Pathology, 33
N. Sonenberg, A. Shatkin, R. Ricciardi, M. Rubin, R. Goodman (1978)
Analysis of terminal structures of RNA from potato virus X.Nucleic acids research, 5 7
W. Dawson, D. Lewandowski, M. Hilf, P. Bubrick, A. Raffo, J. Shaw, G. Grantham, P. Desjardins (1989)
A tobacco mosaic virus-hybrid expresses and loses an added gene.Virology, 172 1
David Beck, P. Guilford, Davin Voot, Mark Andersen, Richard Forster (1991)
Triple gene block proteins of white clover mosaic potexvirus are required for transport.Virology, 183 2
R. Jefferson, T. Kavanagh, M. Bevan (1987)
GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants.The EMBO Journal, 6
V. Dolja, D. Grama, S. Morozov, J. Atabekov (1987)
Potato virus X‐related single‐ and double‐stranded RNAsFEBS Letters, 214
R. Hayes, R. Coutts, K. Buck (1989)
Stability and expression of bacterial genes in replicating geminivirus vectors in plants.Nucleic acids research, 17 7
C. Brough, R. Hayes, A. Morgan, R. Coutts, K. Buck (1988)
Effects of Mutagenesis in vitro on the Ability of Cloned Tomato Golden Mosaic Virus DNA to Infect Nicotiana benthamiana PlantsJournal of General Virology, 69
R. Hull (1990)
The Use and Misuse of Viruses in Cloning and Expression in Plants
N. Brisson, Jerzy Paszkowski, J. Penswick, B. Gronenborn, Ingo Potrykus, Thomas Hohn (1984)
Expression of a bacterial gene in plants by using a viral vectorNature, 310
W. Dawson, P. Bubrick, G. Grantham (1988)
Modifications of the tobacco mosaic virus coat protein gene affecting replication, movement, and symptomatologyPhytopathology, 78
I. Petty, A. Jackson (1990)
Mutational analysis of barley stripe mosaic virus RNA beta.Virology, 179 2
Gabriel Iturriaga, Richard Jefferson, Michael Bevan (1989)
Endoplasmic reticulum targeting and glycosylation of hybrid proteins in transgenic tobacco.The Plant cell, 1
R. French, M. Janda, P. Ahlquist (1986)
Bacterial Gene Inserted in an Engineered RNA Virus: Efficient Expression in Monocotyledonous Plant CellsScience, 231
R. Jefferson, S. Burgess, D. Hirsh (1986)
beta-Glucuronidase from Escherichia coli as a gene-fusion marker.Proceedings of the National Academy of Sciences of the United States of America, 83 22
R. Hayes, I.T.D. Petty, I.T.D. Petty, Robert Coutts, K. Buck (1988)
Gene amplification and expression in plants by a replicating geminivirus vectorNature, 334
J. Bol, L. Vloten-Doting, E. Jaspars (1971)
A functional equivalence of top component a RNA and coat protein in the initiation of infection by alfalfa mosaic virus.Virology, 46 1
R. Joshi, V. Joshi, D. Ow (1990)
BSMV genome mediated expression of a foreign gene in dicot and monocot plant cells.The EMBO Journal, 9
J. Donson, Christopher Kearney, M. Hilf, William Dawson (1991)
Systemic expression of a bacterial gene by a tobacco mosaic virus-based vector.Proceedings of the National Academy of Sciences of the United States of America, 88
K. Skryabin, A. Kraev, Morozov SYu, M. Rozanov, B. Chernov, L. Lukasheva, J. Atabekov (1988)
The nucleotide sequence of potato virus X RNA.Nucleic acids research, 16 22
M. Bradford (1976)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical biochemistry, 72
B. Orman, R. Celnik, A. Mandel, H. Torres, A. Mentaberry (1990)
Complete cDNA sequence of a South American isolate of potato virus X.Virus research, 16 3
P. Ahlquist, R. Pacha (1990)
Gene amplification and expression by RNA viruses and potential for further application to plant gene transferPhysiologia Plantarum, 79
N. Takamatsu, M. Ishikawa, T. Meshi, Y. Okada (1987)
Expression of bacterial chloramphenicol acetyltransferase gene in tobacco plants mediated by TMV‐RNAThe EMBO Journal, 6
Andrew Ward, P. Etessami, J. Stanley (1988)
Expression of a bacterial gene in plants mediated by infectious geminivirus DNAThe EMBO Journal, 7
G. Murphy, T. Kavanagh (1988)
Speeding-up the sequencing of double-stranded DNA.Nucleic acids research, 16 11
R. French, P. Ahlquist (1988)
Characterization and engineering of sequences controlling in vivo synthesis of brome mosaic virus subgenomic RNAJournal of Virology, 62
D. Lefebvre, B. Miki, J. Laliberté (1987)
Mammalian Metallothionein Functions in PlantsBio/Technology, 5
Konstantin Skryabin, S. Morozov, A. Kraev, M. Rozanov, B. Chernov, Lukasheva Li, J. Atabekov (1988)
Conserved and variable elements in RNA genomes of potexvirusesFEBS Letters, 240
The suitability of potato virus X (PVX) as a gene vector in plants was tested by analysis of two viral constructs. In the first, the GUS gene of Escherichia coli was substituted for the viral coat protein gene. In the second, GUS was added into the viral genome coupled to a duplicated copy of the viral promoter for the coat protein mRNA. The viral construct with the substituted coat protein gene accumulated poorly in inoculated protoplasts and failed to spread from the site of infection in plants. These results suggest a role for the viral coat protein in key stages of the viral infection cycle and show that gene replacement constructs are not suitable for the production of PVX‐based gene vector. The construct with GUS coupled to the duplicated promoter for coat protein mRNA also accumulated less well in protoplasts than the unmodified PVX, but did infect systemically and directed high level synthesis of GUS in inoculated and systemically infected tissue. Although there was some genome instability in the PVX construct, much of the viral RNA in the systemically infected tissue had retained the foreign gene insertion, especially in infected Nicotiana clevelandii plants. These data point to a general utility of PVX as a vector for unregulated gene expression in plants.
The Plant Journal – Wiley
Published: Jul 1, 1992
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