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Enhanced oxidative-stress defense in transgenic potato expressing tomato Cu,Zn superoxide dismutases

Enhanced oxidative-stress defense in transgenic potato expressing tomato Cu,Zn superoxide dismutases 122 85 85 5 5 A. Perl R. Perl-Treves S. Galili D. Aviv E. Shalgi S. Malkin E. Galun Department of Plant Genetics Weizmann Institute of Science 76100 Rehovot Israel Department of Biochemistry The Weizmann Institute of Science 76100 Rehovot Israel Summary The two cDNAs coding for the cytosolic ( cyt ) and the chloroplast-located ( chl ) Cu,Zn superoxide dismutases (SODs) of tomato (Perl-Treves et al. 1988) were cloned into respective binary vectors and mobilized into Agrobacterium strains. Potato tuber discs were infected with either of the two agrobacterial strains and cultured on selective medium containing kanaymcin. The integration of either of the cyt or the chl SOD transgenes was verified by Southern-blot hybridization. The enzymatic activity of the additional tomato chl Cu,Zn SOD could be distinguished from endogenous SOD activity since the latter isozyme migrated faster on SOD-activity gels. Several transgenic potato lines harboring either the cyt or the chl SOD genes of tomato showed elevated tolerance to the superoxide-generating herbicide paraquat (methyl viologen). After exposure of shoots to paraquat, tolerance was recorded either by scoring symptoms visually or by measurements of photosynthesis using the photoacoustic method. Root cultures from transgenic lines that harbored the additional cyt Cu,Zn SOD gene of tomato were tolerant to methyl viologen up to 10 -5 M; a lower tolerance was recorded in roots of transgenic lines that expressed the additional chl Cu,Zn SOD of tomato. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png TAG Theoretical and Applied Genetics Springer Journals

Enhanced oxidative-stress defense in transgenic potato expressing tomato Cu,Zn superoxide dismutases

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References (55)

Publisher
Springer Journals
Copyright
Copyright © 1993 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Agriculture; Biochemistry, general; Plant Biochemistry; Plant Sciences; Plant Genetics & Genomics
ISSN
0040-5752
eISSN
1432-2242
DOI
10.1007/BF00220915
pmid
24195931
Publisher site
See Article on Publisher Site

Abstract

122 85 85 5 5 A. Perl R. Perl-Treves S. Galili D. Aviv E. Shalgi S. Malkin E. Galun Department of Plant Genetics Weizmann Institute of Science 76100 Rehovot Israel Department of Biochemistry The Weizmann Institute of Science 76100 Rehovot Israel Summary The two cDNAs coding for the cytosolic ( cyt ) and the chloroplast-located ( chl ) Cu,Zn superoxide dismutases (SODs) of tomato (Perl-Treves et al. 1988) were cloned into respective binary vectors and mobilized into Agrobacterium strains. Potato tuber discs were infected with either of the two agrobacterial strains and cultured on selective medium containing kanaymcin. The integration of either of the cyt or the chl SOD transgenes was verified by Southern-blot hybridization. The enzymatic activity of the additional tomato chl Cu,Zn SOD could be distinguished from endogenous SOD activity since the latter isozyme migrated faster on SOD-activity gels. Several transgenic potato lines harboring either the cyt or the chl SOD genes of tomato showed elevated tolerance to the superoxide-generating herbicide paraquat (methyl viologen). After exposure of shoots to paraquat, tolerance was recorded either by scoring symptoms visually or by measurements of photosynthesis using the photoacoustic method. Root cultures from transgenic lines that harbored the additional cyt Cu,Zn SOD gene of tomato were tolerant to methyl viologen up to 10 -5 M; a lower tolerance was recorded in roots of transgenic lines that expressed the additional chl Cu,Zn SOD of tomato.

Journal

TAG Theoretical and Applied Genetics – Springer Journals

Published: Jan 1, 1993

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