Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/wiley/in-vitro-evaluation-of-the-biological-performance-of-macro-micro-mVlAQ0Ud8V
References (43)
-
Yufeng Zhang, Chengtie Wu, T. Friis, Yin Xiao (2010)
The osteogenic properties of CaP/silk composite scaffolds.Biomaterials, 31 10
-
Charu Vepari, D. Kaplan (2007)
Silk as a Biomaterial.Progress in polymer science, 32 8-9
-
W. Grayson, P. Chao, Darja Marolt, D. Kaplan, G. Vunjak‐Novakovic (2008)
Engineering custom-designed osteochondral tissue grafts.Trends in biotechnology, 26 4
-
V. Karageorgiou, D. Kaplan (2005)
Porosity of 3D biomaterial scaffolds and osteogenesis.Biomaterials, 26 27
-
Sang‐Soo Kim, Min Park, Oju Jeon, Cha Choi, Byung‐Soo Kim (2006)
Poly(lactide-co-glycolide)/hydroxyapatite composite scaffolds for bone tissue engineering.Biomaterials, 27 8
-
B. Mandal, S. Kundu (2009)
Cell proliferation and migration in silk fibroin 3D scaffolds.Biomaterials, 30 15
-
A. Lammel, Xiao Hu, Sang-Hyug Park, D. Kaplan, T. Scheibel (2010)
Controlling silk fibroin particle features for drug delivery.Biomaterials, 31 16
-
P. Malafaya, G. Silva, R. Reis (2007)
Natural-origin polymers as carriers and scaffolds for biomolecules and cell delivery in tissue engineering applications.Advanced drug delivery reviews, 59 4-5
-
G. Altman, Frank Diaz, Caroline Jakuba, T. Calabro, Rebecca Horan, Jingsong Chen, Helen Lu, J. Richmond, D. Kaplan (2003)
Silk-based biomaterials.Biomaterials, 24 3
-
C. Murphy, M. Haugh, F. O'Brien (2010)
The effect of mean pore size on cell attachment, proliferation and migration in collagen-glycosaminoglycan scaffolds for bone tissue engineering.Biomaterials, 31 3
-
P. Malafaya, A. Pedro, A. Peterbauer, Christian Gabriel, H. Redl, Rui Reis (2005)
Chitosan particles agglomerated scaffolds for cartilage and osteochondral tissue engineering approaches with adipose tissue derived stem cellsJournal of Materials Science: Materials in Medicine, 16
-
A. Duarte, J. Mano, R. Reis (2009)
Supercritical fluids in biomedical and tissue engineering applications: a reviewInternational Materials Reviews, 54
-
A. Collins, N. Skaer, T. Gheysens, D. Knight, C. Bertram, H. Roach, R. Oreffo, Sonja Von-Aulock, Teodora Baris, J. Skinner, S. Mann (2009)
Bone‐like Resorbable Silk‐based Scaffolds for Load‐bearing Osteoregenerative ApplicationsAdvanced Materials, 21
-
Le-Ping Yan, A. Salgado, J. Oliveira, Ana Oliveira, R. Reis (2013)
De novo bone formation on macro/microporous silk and silk/nano-sized calcium phosphate scaffoldsJournal of Bioactive and Compatible Polymers, 28
-
Sarindr Bhumiratana, W. Grayson, A. Castaneda, D. Rockwood, E. Gil, D. Kaplan, G. Vunjak‐Novakovic (2011)
Nucleation and growth of mineralized bone matrix on silk-hydroxyapatite composite scaffolds.Biomaterials, 32 11
-
Le-Ping Yan, Yingjun Wang, L. Ren, Gang Wu, S. Caridade, Jia-Bing Fan, Lingying Wang, P. Ji, J. Oliveira, J. Oliveira, J. Mano, R. Reis (2010)
Genipin-cross-linked collagen/chitosan biomimetic scaffolds for articular cartilage tissue engineering applications.Journal of biomedical materials research. Part A, 95 2
-
R. Nazarov, H. Jin, D. Kaplan (2004)
Porous 3-D scaffolds from regenerated silk fibroin.Biomacromolecules, 5 3
-
D. Hutmacher (2000)
Scaffolds in tissue engineering bone and cartilage.Biomaterials, 21 24
-
A. Murphy, D. Kaplan (2009)
Biomedical applications of chemically-modified silk fibroin.Journal of materials chemistry, 19 36
-
S. Oh, I. Park, Jin Kim, J. Lee (2007)
In vitro and in vivo characteristics of PCL scaffolds with pore size gradient fabricated by a centrifugation method.Biomaterials, 28 9
-
J. Guan, K. Fujimoto, M. Sacks, W. Wagner (2005)
Preparation and characterization of highly porous, biodegradable polyurethane scaffolds for soft tissue applications.Biomaterials, 26 18
-
A. Motta, C. Migliaresi, F. Faccioni, P. Torricelli, M. Fini, R. Giardino (2004)
Fibroin hydrogels for biomedical applications: preparation, characterization and in vitro cell culture studiesJournal of Biomaterials Science, Polymer Edition, 15
-
J. Elisseeff (2004)
Injectable cartilage tissue engineeringExpert Opinion on Biological Therapy, 4
-
Langer (1993)
Tissue engineeringScience, 260
-
H. Jin, Jaehyung Park, V. Karageorgiou, Ung-Jin Kim, R. Valluzzi, P. Cebe, D. Kaplan (2005)
Water‐Stable Silk Films with Reduced β‐Sheet ContentAdvanced Functional Materials, 15
-
C. Agrawal, Robert Ray (2001)
Biodegradable polymeric scaffolds for musculoskeletal tissue engineering.Journal of biomedical materials research, 55 2
-
J. Oliveira, S. Silva, P. Malafaya, M. Rodrigues, N. Kotobuki, M. Hirose, M. Gomes, J. Mano, H. Ohgushi, R. Reis (2009)
Macroporous hydroxyapatite scaffolds for bone tissue engineering applications: physicochemical characterization and assessment of rat bone marrow stromal cell viability.Journal of biomedical materials research. Part A, 91 1
-
D. Hutmacher, J. Schantz, C. Lam, K. Tan, T. Lim (2007)
State of the art and future directions of scaffold‐based bone engineering from a biomaterials perspectiveJournal of Tissue Engineering and Regenerative Medicine, 1
-
D. Hutmacher, M. Sittinger, M. Risbud (2004)
Scaffold-based tissue engineering: rationale for computer-aided design and solid free-form fabrication systems.Trends in biotechnology, 22 7
-
C. Correia, Sarindr Bhumiratana, Le-Ping Yan, A. Oliveira, J. Gimble, D. Rockwood, D. Kaplan, R. Sousa, R. Reis, G. Vunjak‐Novakovic (2012)
Development of silk-based scaffolds for tissue engineering of bone from human adipose-derived stem cells.Acta biomaterialia, 8 7
-
Le-Ping Yan, J. Silva-Correia, C. Correia, S. Caridade, E. Fernandes, R. Sousa, J. Mano, J. Oliveira, A. Oliveira, R. Reis (2013)
Bioactive macro/micro porous silk fibroin/nano-sized calcium phosphate scaffolds with potential for bone-tissue-engineering applications.Nanomedicine, 8 3
-
A. Oliveira, Lin Sun, Hyeon-Joo Kim, Xiao Hu, William Rice, J. Kluge, R. Reis, D. Kaplan (2012)
Aligned silk-based 3-D architectures for contact guidance in tissue engineering.Acta biomaterialia, 8 4
-
Yongzhong Wang, D. Blasioli, Hyeon-Joo Kim, Hyun Kim, D. Kaplan (2006)
Cartilage tissue engineering with silk scaffolds and human articular chondrocytes.Biomaterials, 27 25
-
S. Abbah, C. Lam, D. Hutmacher, J. Goh, H. Wong (2009)
Biological performance of a polycaprolactone-based scaffold used as fusion cage device in a large animal model of spinal reconstructive surgery.Biomaterials, 30 28
-
A. Salgado, O. Coutinho, R. Reis (2004)
Bone tissue engineering: state of the art and future trends.Macromolecular bioscience, 4 8
-
Joaquim Oliveira, Joaquim Oliveira, N. Kotobuki, M. Tadokoro, M. Hirose, J. Mano, Rui Reis, H. Ohgushi (2010)
Ex vivo culturing of stromal cells with dexamethasone-loaded carboxymethylchitosan/poly(amidoamine) dendrimer nanoparticles promotes ectopic bone formation.Bone, 46 5
-
U. Kim, Jaehyung Park, Hyeon-Joo Kim, M. Wada, D. Kaplan (2005)
Three-dimensional aqueous-derived biomaterial scaffolds from silk fibroin.Biomaterials, 26 15
-
Le-Ping Yan, J. Oliveira, A. Oliveira, S. Caridade, J. Mano, R. Reis (2012)
Macro/microporous silk fibroin scaffolds with potential for articular cartilage and meniscus tissue engineering applications.Acta biomaterialia, 8 1
-
Hyeon-Joo Kim, Sang-Hyug Park, Jennah Durham, J. Gimble, D. Kaplan, J. Dragoo (2012)
In vitro chondrogenic differentiation of human adipose-derived stem cells with silk scaffoldsJournal of Tissue Engineering, 3
-
Nandana Bhardwaj, S. Kundu (2012)
Chondrogenic differentiation of rat MSCs on porous scaffolds of silk fibroin/chitosan blends.Biomaterials, 33 10
-
R. Langer, J. Vacanti (1993)
Tissue engineering : Frontiers in biotechnologyScience, 260
-
X. Kong, F. Cui, Xiaohao Wang, M. Zhang, Wei Zhang (2004)
Silk fibroin regulated mineralization of hydroxyapatite nanocrystalsJournal of Crystal Growth, 270
-
Hyeon-Joo Kim, U. Kim, G. Leisk, Christopher Bayan, I. Georgakoudi, D. Kaplan (2007)
Bone regeneration on macroporous aqueous-derived silk 3-D scaffolds.Macromolecular bioscience, 7 5
- Publisher
- Wiley
- Copyright
- © 2015 Wiley Periodicals, Inc.
- ISSN
- 1549-3296
- eISSN
- 1552-4965
- DOI
- 10.1002/jbm.b.33267
- pmid
- 25164158
- Publisher site
- See Article on Publisher Site
Abstract
This study evaluates the biological performance of salt‐leached macro/microporous silk scaffolds (S16) and silk‐nano calcium phosphate scaffolds (SC16), both deriving from a 16 wt % aqueous SF solution. Enzymatic degradation results showed that the silk‐based scaffolds presented desirable biostability, and the incorporation of calcium phosphate further improved the scaffolds' biostability. Human adipose tissue derived stromal cells (hASCs) were cultured onto the scaffolds in vitro. The Alamar blue assay and DNA content revealed that both scaffolds were non‐cytotoxic and can support the viability and proliferation of the hASCs. Scanning electron microscopy observation demonstrated that the microporous structure was beneficial for the cell adhesion while the macroporous structure favored the cell migration and proliferation. The histological analysis displayed abundant extracellular matrix formed inside the scaffolds, leading to the significant increase of scaffolds' modulus. These results revealed that S16 and SC16 could be promising alternatives for cartilage and bone tissue engineering scaffolding applications, respectively. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 888–898, 2015.
Journal
Journal of Biomedical Materials Research – Wiley
Published: May 1, 2015