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Development of a cell line from primary cultures of rainbow trout, Oncorhynchus mykiss (Walbaum), gills

Development of a cell line from primary cultures of rainbow trout, Oncorhynchus mykiss (Walbaum),... Abstract. Collagenase was used to prepare primary cell cultures from rainbow trout, Oncorhynchus mykiss (Walbaum), gills. Although difficult to subcultivate, one primary culture led to the development of a cell line, RTgill‐W1. RTgill‐W1 grew in the basal medium, L‐15, supplemented with foetal bovine serum at between 5 and 10%, but not in L‐15 alone. The cells have been passaged approximately 50 times over a 4‐year period. At confluency, the cell shape was predominantly polygonal or epithelial‐like. RTgill‐Wl cultures were demonstrated by DNA staining with H33258 and by growth on agar to be contaminated with mycoplasma, but this contaminant was eradicated by treatment with mycoplasma removal agent (MRA) and BM cyclin. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Fish Diseases Wiley

Development of a cell line from primary cultures of rainbow trout, Oncorhynchus mykiss (Walbaum), gills

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References (19)

Publisher
Wiley
Copyright
Copyright © 1994 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0140-7775
eISSN
1365-2761
DOI
10.1111/j.1365-2761.1994.tb00258.x
Publisher site
See Article on Publisher Site

Abstract

Abstract. Collagenase was used to prepare primary cell cultures from rainbow trout, Oncorhynchus mykiss (Walbaum), gills. Although difficult to subcultivate, one primary culture led to the development of a cell line, RTgill‐W1. RTgill‐W1 grew in the basal medium, L‐15, supplemented with foetal bovine serum at between 5 and 10%, but not in L‐15 alone. The cells have been passaged approximately 50 times over a 4‐year period. At confluency, the cell shape was predominantly polygonal or epithelial‐like. RTgill‐Wl cultures were demonstrated by DNA staining with H33258 and by growth on agar to be contaminated with mycoplasma, but this contaminant was eradicated by treatment with mycoplasma removal agent (MRA) and BM cyclin.

Journal

Journal of Fish Diseases – Wiley

Published: Nov 1, 1994

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