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Different Potential of Cellular and Viral Activators of Transcription Revealed in Oocytes and Early Embryos of Xenopus laevis

Different Potential of Cellular and Viral Activators of Transcription Revealed in Oocytes and... Biol.Chem. Hoppe-Seyler, Vol. 375, pp. 105-112, February 1994 · Copyright ©by Walter de Gruyter& Co· Berlin · New York Licen Xu, Duri Rungger1, Oleg Georgiev, Katja Seipel and Walter Schaffner* Institut für Molekularbiologie II, Universität Zürich, Winterthurerstr. 190, CH-8057 Zürich, Switzerland 1 Station de Zoologie experimental, University of Geneva, 154 route de Malagnou, CH-1224 Chene-Bougeries, Switzerland * Corresponding author Introduction Microinjection into oocytes or fertilized eggs of the african clawed toad Xenopus laevis has long been used to study transcription processes (Mous et al., 1985; Gurdon and Wakefield, 1986 and references therein). In the oocyte, there is generally high transcriptional activity, which attenuates as genes are inactivated upon maturation towards the egg. After fertilization, Xenopus zygotes undergo 12 synchronous rapid divisions which are followed, after a so-called midblastula transition (MBT), by slower and asynchronous cell proliferation. At midblastula transition, RNA polymerases II and III begin to transcribe genes, and cells become motile (Newport and Kirschner, 1982; Kimelman et al, 1987). Promoters of injected genes are subject to the same timing of expression as the endogenous genes in both amphibian and mouse (Gurdon and Wakefield, 1986; Majumder et al., 1993). The function of two classes of c/s-acting transcriptional control http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biological Chemistry Hoppe-Seyler de Gruyter

Different Potential of Cellular and Viral Activators of Transcription Revealed in Oocytes and Early Embryos of Xenopus laevis

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References (5)

Publisher
de Gruyter
Copyright
Copyright © 1994 by the
ISSN
0177-3593
eISSN
1437-4315
DOI
10.1515/bchm3.1994.375.2.105
Publisher site
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Abstract

Biol.Chem. Hoppe-Seyler, Vol. 375, pp. 105-112, February 1994 · Copyright ©by Walter de Gruyter& Co· Berlin · New York Licen Xu, Duri Rungger1, Oleg Georgiev, Katja Seipel and Walter Schaffner* Institut für Molekularbiologie II, Universität Zürich, Winterthurerstr. 190, CH-8057 Zürich, Switzerland 1 Station de Zoologie experimental, University of Geneva, 154 route de Malagnou, CH-1224 Chene-Bougeries, Switzerland * Corresponding author Introduction Microinjection into oocytes or fertilized eggs of the african clawed toad Xenopus laevis has long been used to study transcription processes (Mous et al., 1985; Gurdon and Wakefield, 1986 and references therein). In the oocyte, there is generally high transcriptional activity, which attenuates as genes are inactivated upon maturation towards the egg. After fertilization, Xenopus zygotes undergo 12 synchronous rapid divisions which are followed, after a so-called midblastula transition (MBT), by slower and asynchronous cell proliferation. At midblastula transition, RNA polymerases II and III begin to transcribe genes, and cells become motile (Newport and Kirschner, 1982; Kimelman et al, 1987). Promoters of injected genes are subject to the same timing of expression as the endogenous genes in both amphibian and mouse (Gurdon and Wakefield, 1986; Majumder et al., 1993). The function of two classes of c/s-acting transcriptional control

Journal

Biological Chemistry Hoppe-Seyler – de Gruyter

Published: Feb 1, 1994

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