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Isolation and characterization of a thermostable cellulase-producing Fusarium chlamydosporum

Isolation and characterization of a thermostable cellulase-producing Fusarium chlamydosporum A thermostable cellulase-producing fungus, HML 0278, was identified as Fusarium chlamydosporum by morphological characteristics and ITS rDNA sequence analysis. HML 0278 produced extracellular cellulases in solid-state fermentation using sugar cane bassage as the carbon source. Native-PAGE analysis demonstrated that this fungus strain was capable of producing the three major components of cellulases and xylanase, with a yield of 281.8 IU/g for CMCase, 182.4 IU/g for cellobiohydrolase, 135.2 IU/g for β-glucosidase, 95.2 IU/g for filter paper activity, and 4,720 IU/g for xylanase. More importantly, the CMCase and β-glucosidase produced by HML 0278 showed stable enzymatic activities within pH 4–9 and pH 4–10, and at temperatures below 70 and 60°C, respectively. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png World Journal of Microbiology and Biotechnology Springer Journals

Isolation and characterization of a thermostable cellulase-producing Fusarium chlamydosporum

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References (31)

Publisher
Springer Journals
Copyright
Copyright © 2010 by Springer Science+Business Media B.V.
Subject
Chemistry; Microbiology ; Environmental Engineering/Biotechnology; Applied Microbiology; Biochemistry, general; Biotechnology
ISSN
0959-3993
eISSN
1573-0972
DOI
10.1007/s11274-010-0383-x
Publisher site
See Article on Publisher Site

Abstract

A thermostable cellulase-producing fungus, HML 0278, was identified as Fusarium chlamydosporum by morphological characteristics and ITS rDNA sequence analysis. HML 0278 produced extracellular cellulases in solid-state fermentation using sugar cane bassage as the carbon source. Native-PAGE analysis demonstrated that this fungus strain was capable of producing the three major components of cellulases and xylanase, with a yield of 281.8 IU/g for CMCase, 182.4 IU/g for cellobiohydrolase, 135.2 IU/g for β-glucosidase, 95.2 IU/g for filter paper activity, and 4,720 IU/g for xylanase. More importantly, the CMCase and β-glucosidase produced by HML 0278 showed stable enzymatic activities within pH 4–9 and pH 4–10, and at temperatures below 70 and 60°C, respectively.

Journal

World Journal of Microbiology and BiotechnologySpringer Journals

Published: Mar 30, 2010

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