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Michael Miller, C. Seighman, R. Ulrich (1985)
Inhibition of DNA replication and DNA polymerase alpha activity by monoclonal anti-(DNA polymerase alpha) immunoglobulin G and F(ab) fragments.Biochemistry, 24 25
Ross TuboS, Ronald Berezneyg (1987)
Identification of 100 and 150 S DNA polymerase alpha-primase megacomplexes solubilized from the nuclear matrix of regenerating rat liver.The Journal of biological chemistry, 262 12
V. Mikhailov, R. Tsanev (1983)
intranuclear localization of dna polymerases α and β in regenerating rat liverInternational Journal of Biochemistry, 15
P. Madsen, J. Celis (1985)
S‐phase patterns of cyclin (PCNA) antigen staining resemble topographical patterns of DNA synthesisFEBS Letters, 193
R. Painter, B. Young (1976)
Formation of nascent DNA molecules during inhibition of replicon initiation in mammalian cells.Biochimica et biophysica acta, 418 2
(1977)
Semi-conservative DNA replication in vitro
M. Nishizawa, K. Tanabe, T. Takahashi (1984)
DNA polymerases and DNA topoisomerases solubilized from nuclear matrices of regenerating rat livers.Biochemical and biophysical research communications, 124 3
H. Gratzner (1982)
Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication.Science, 218 4571
R. Hand (1975)
Regulation of DNA replication on subchromosomal units of mammalian cellsThe Journal of Cell Biology, 64
Peter Reinhard, Marianne Burkhalter, J. Gautschi (1977)
Semiconservative DNA replication in vitro. I. Properties of two systems derived from mouse P-815 cells by permeabilization or lysis with Brij-58.Biochimica et biophysica acta, 474 4
R. Bravo, R. Frank, P. Blundell, H. Macdonald-Bravo (1987)
Cyclin/PCNA is the auxiliary protein of DNA polymerase-δNature, 326
H. Velden, M. Poot, F. Wanka (1984)
In vitro DNA replication in association with the nuclear matrix of permeable mammalian cells.Biochimica et biophysica acta, 782 4
R. Bravo (1987)
Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle: association with DNA replication sitesThe Journal of Cell Biology, 105
M. Pardue, J. Gall (1970)
Chromosomal Localization of Mouse Satellite DNAScience, 168
G. Holmquist, M. Gray, T. Porter, John Jordan (1982)
Characterization of Giemsa dark- and light-band DNACell, 31
MA Goldman, Gerald Holmquist, MC Gray, LA Caston, A. Nag (1984)
Replication timing of genes and middle repetitive sequences.Science, 224 4650
H. Smith, R. Berezney (1980)
DNA polymerase alpha is tightly bound to the nuclear matrix of actively replicating liver.Biochemical and biophysical research communications, 97 4
R. Tubo, R. Berezney (1987)
Pre-replicative association of multiple replicative enzyme activities with the nuclear matrix during rat liver regeneration.The Journal of biological chemistry, 262 3
R. Berezney, L. Buchholtz (1981)
Dynamic association of replicating DNA fragments with the nuclear matrix of regenerating liver.Experimental cell research, 132 1
M. Staufenbiel, W. Deppert (1984)
Preparation of nuclear matrices from cultured cells: subfractionation of nuclei in situThe Journal of Cell Biology, 98
M. Gefter (1975)
DNA replication.Annual review of biochemistry, 44
J. Gautschi, Marianne Burkhalter, Peter Reinhard (1977)
Semiconservative DNA replication in vitro. II. Replicative intermediates of mouse P-815 cells.Biochimica et biophysica acta, 474 4
S. Wood, J. Collins (1986)
Preferential binding of DNA primase to the nuclear matrix in HeLa cells.The Journal of biological chemistry, 261 16
S. Yamamoto, T. Takahashi, A. Matsukage (1984)
Tight association of DNA polymerase alpha with granular structures in the nuclear matrix of chick embryo cell: immunocytochemical detection with monoclonal antibody against DNA polymerase alpha.Cell structure and function, 9 1
R. Tubo, R. Berezney (1987)
Nuclear matrix-bound DNA primase. Elucidation of an RNA priming system in nuclear matrix isolated from regenerating rat liver.The Journal of biological chemistry, 262 14
H. Velden, Gijsbert Willigen, R. Wetzels, F. Wanka (1984)
Attachment of origins of replication to the nuclear matrix and the chromosomal scaffoldFEBS Letters, 171
R. Painter (1978)
Inhibition of DNA replicon initiation by 4-nitroquinoline 1-oxide, adriamycin, and ethyleneimine.Cancer research, 38 12
(1978)
High resolution autoradiography studies on chromatin functions
HaroldC. Smith, R. Berezney (1980)
DNA polymerase α is tightly bound to the nuclear matrix of actively replicating liverBiochemical and Biophysical Research Communications, 97
B. Vogelstein, D. Pardoll, D. Coffey (1980)
Supercoiled loops and eucaryotic DNA replicationCell, 22
R. Jalouzot, B. Toublan, M. Wilhelm, F. Wilhelm (1985)
Replication timing of the H4 histone genes in Physarum polycephalum.Proceedings of the National Academy of Sciences of the United States of America, 82 19
(1981)
Studies of mammalian chromosome replication. II. Evidence for the existence of define chromosome replicating units
M. Marcus, A. Fainsod, G. Diamond (1985)
The genetic analysis of mammalian cell-cycle mutants.Annual review of genetics, 19
G. Pierron, D. Durica, H. Sauer (1984)
Invariant temporal order of replication of the four actin gene loci during the naturally synchronous mitotic cycles of Physarum polycephalum.Proceedings of the National Academy of Sciences of the United States of America, 81 20
(1983)
Intranuclear localization ofDNA
R. Tubo, H. Smith, R. Berezney (1985)
The nuclear matrix continues DNA synthesis at in vivo replicational forks.Biochimica et biophysica acta, 825 3
A. Mullinger, T. Robert, Johnson (1983)
Units of chromosome replication and packing.Journal of cell science, 64
R. Bravo, H. Macdonald-Bravo (1985)
Changes in the nuclear distribution of cyclin (PCNA) but not its synthesis depend on DNA replication.The EMBO Journal, 4
G. Milner (1969)
Nuclear morphology and the ultrastructural localization of deoxyribonucleic acid synthesis during interphase.Journal of cell science, 4 3
E. Fey, G. Krochmalnic, S. Penman (1986)
The nonchromatin substructures of the nucleus: the ribonucleoprotein (RNP)-containing and RNP-depleted matrices analyzed by sequential fractionation and resinless section electron microscopyThe Journal of Cell Biology, 102
R. Hand, J. Gautschi (1979)
Replication of mammalian DNA in vitro. Evidence for initiation from fiber autoradiographyThe Journal of Cell Biology, 82
(1988)
for publication 29 March 1988, and in revised form 16
(1981)
Proc. Natl. Acad. Sci. USA
H. Smith, R. Berezney (1982)
Nuclear matrix-bound deoxyribonucleic acid synthesis: an in vitro system.Biochemistry, 21 26
D. Capco, K. Wan, S. Penman (1982)
The nuclear matrix: Three-dimensional architecture and protein compositionCell, 29
J. Collins, A. Chu (1987)
Binding of the DNA polymerase alpha-DNA primase complex to the nuclear matrix in HeLa cells.Biochemistry, 26 18
H. Smith, E. Puvion, L. Buchholtz, R. Berezney (1984)
Spatial distribution of DNA loop attachment and replicational sites in the nuclear matrixThe Journal of Cell Biology, 99
S. McCready, J. Godwin, D. Mason, I. Brazell, P. Cook (1980)
DNA is replicated at the nuclear cage.Journal of cell science, 46
R. Hand (1978)
Eucaryotic DNA: Organization of the genome for replicationCell, 15
M. Carrì, G. Micheli, E. Graziano, T. Pace, M. Buongiorno-Nardelli (1986)
The relationship between chromosomal origins of replication and the nuclear matrix during the cell cycle.Experimental cell research, 164 2
H. Nakamura, T. Morita, S. Masaki, S. Yoshida (1984)
Intracellular localization and metabolism of DNA polymerase alpha in human cells visualized with monoclonal antibody.Experimental cell research, 151 1
S. Latt (1975)
Fluorescence analysis of late DNA replication in human metaphase chromosomesSomatic Cell Genetics, 1
P. Dijkwel, P. Wenink, J. Poddighe (1986)
Permanent attachment of replication origins to the nuclear matrix in BHK-cells.Nucleic acids research, 14 8
N. Berger, S. Petzold, E. Johnson (1977)
High molecular weight DNA intermediates synthesized by permeabilized L cells.Biochimica et biophysica acta, 478 1
S. Fakan, R. Hancock (1974)
Localization of newly-synthesized DNA in a mammalian cell as visualized by high resolution autoradiography.Experimental cell research, 83 1
D. Gilbert (1986)
Temporal order of replication of Xenopus laevis 5S ribosomal RNA genes in somatic cells.Proceedings of the National Academy of Sciences of the United States of America, 83 9
P. Dijkwel, L. Mullenders, F. Wanka (1979)
Analysis of the attachment of replicating DNA to a nuclear matrix in mammalian interphase nucleiNucleic acids research, 6 1
Nakayasu and Berezney Replicational Sites in the Cell Nucleus I 1
D. Pardoll, B. Vogelstein, D. Coffey (1980)
A fixed site of DNA replication in eucaryotic cellsCell, 19
D. Olins, A. Olins (1987)
In vitro DNA synthesis in the macronuclear replication band of Euplotes eurystomusThe Journal of Cell Biology, 104
P. Langer, A. Waldrop, D. Ward (1981)
Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.Proceedings of the National Academy of Sciences of the United States of America, 78 11
(1984)
A in vitro system. Biochemistry
R. Hand, Igor Tamm (1974)
Initiation of DNA replication in mammalian cells and its inhibition by reovirus infection.Journal of molecular biology, 82 2
J. Celis, A. Celis (1985)
Cell cycle-dependent variations in the distribution of the nuclear protein cyclin proliferating cell nuclear antigen in cultured cells: subdivision of S phase.Proceedings of the National Academy of Sciences of the United States of America, 82 10
Ronald Berezney, D. Coffey (1975)
Nuclear protein matrix: association with newly synthesized DNA.Science, 189 4199
D. Jackson, P. Cook (1986)
A cell-cycle-dependent DNA polymerase activity that replicates intact DNA in chromatin.Journal of molecular biology, 192 1
E. Hay, J. Revel (1963)
THE FINE STRUCTURE OF THE DNP COMPONENT OF THE NUCLEUSThe Journal of Cell Biology, 16
K. Foster, J. Collins (1985)
The interrelation between DNA synthesis rates and DNA polymerases bound to the nuclear matrix in synchronized HeLa cells.The Journal of biological chemistry, 260 7
Taka Hori, Karl Lark (1974)
Autoradiographic studies of the replication of satellite DNA in the kangaroo rat. Autoradiographs of satellite DNA.Journal of molecular biology, 88 1
H. Nakamura, T. Morita, C. Sato (1986)
Structural organizations of replicon domains during DNA synthetic phase in the mammalian nucleus.Experimental cell research, 165 2
M. Camargo, J. Červenka (1982)
Patterns of DNA replication of human chromosomes. II. Replication map and replication model.American journal of human genetics, 34 5
C. Jones, R. Su (1982)
DNA polymerase alpha from the nuclear matrix of cells infected with simian virus 40.Nucleic acids research, 10 18
J. Aelen, R. Opstelten, F. Wanka (1983)
Organization of DNA replication in Physarum polycephalum. Attachment of origins of replicons and replication forks to the nuclear matrix.Nucleic acids research, 11 4
D. Jackson, P. Cook (1986)
Replication occurs at a nucleoskeleton.The EMBO Journal, 5
G. Prelich, Cheng Tan, M. Kostura, M. Mathews, A. So, K. Downey, B. Stillman (1987)
Functional identity of proliferating cell nuclear antigen and a DNA polymerase-δ auxiliary proteinNature, 326
M. Valenzuela, G. Mueller, S. Dasgupta (1983)
Nuclear matrix-DNA complex resulting from EcoR1 digestion of HeLa nucleoids is enriched for DNA replicating forks.Nucleic acids research, 11 7
We have used fluorescent microscopy to map DNA replication sites in the interphase cell nucleus after incorporation of biotinylated dUTP into permeabilized PtK-1 kangaroo kidney or 3T3 mouse fibroblast cells. Discrete replication granules were found distributed throughout the nuclear interior and along the periphery. Three distinct patterns of replication sites in relationship to chromatin domains in the cell nucleus and the period of S phase were detected and termed type I (early to mid S), type II (mid to late S) and type III (late S). Similar patterns were seen with in vivo replicated DNA using antibodies to 5-bromodeoxyuridine. Extraction of the permeabilized cells with DNase I and 0.2 M ammonium sulfate revealed a striking maintenance of these replication granules and their distinct intranuclear arrangements with the remaining nuclear matrix structures despite the removal of greater than 90% of the total nuclear DNA. The in situ prepared nuclear matrix structures also incorporated biotinylated dUTP into replication granules that were indistinguishable from those detected within the intact nucleus.
The Journal of Cell Biology – Rockefeller University Press
Published: Jan 1, 1989
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