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Genetic transformation of crop plants using microprojectile bombardment

Genetic transformation of crop plants using microprojectile bombardment Paul Christou* Agracetus Inc., 8520 University Green, Middleton, WI 53562, USA Regeneration of intact plants from transformed tissue is not always an easy task. In a number of systems it is quite straightforward to engineer tissue that is not competent for regeneration(Christou et a/., 1987).Additional barriers include tissue culture-induced variation, time factors for the recovery of transformants, labor intensive protocols, and limitations in regenerating plants from protoplast, callus and suspension cultures. It would be advantageous therefore, to develop efficient transformation methodology which would allow recovery of transgenic plants without the above constraints. Introduction Development of procedures in cell biology to regenerate plants from single cells and organized tissue, and the discovery of novel techniques to transfer genes to plant cells provided the prerequisite for the practical use of genetic engineering in crop improvement. These advances have given us the opportunity to create, characterize and select plant cultivars which could not be obtained by traditional breeding methods. Genetic engineeringof such recalcitrant crops as maize (Fromm et a/., 1990; GordonKamm eta/.,1990), rice (Christou eta/.,1991; Datta eta/., 1990; Toriyama eta/.,1988), cotton (McCabe and Martinell, 1991; Umbeck et a/., 1987), and soybean (Christou et a/., 1990; McCabe et a/., 1988) is now http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Plant Journal Wiley

Genetic transformation of crop plants using microprojectile bombardment

Christou, Paul
The Plant Journal , Volume 2 (3) – May 1, 1992
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References (63)

Publisher
Wiley
Copyright
Copyright © 1992 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0960-7412
eISSN
1365-313X
DOI
10.1111/j.1365-313X.1992.00275.x
Publisher site
See Article on Publisher Site

Abstract

Paul Christou* Agracetus Inc., 8520 University Green, Middleton, WI 53562, USA Regeneration of intact plants from transformed tissue is not always an easy task. In a number of systems it is quite straightforward to engineer tissue that is not competent for regeneration(Christou et a/., 1987).Additional barriers include tissue culture-induced variation, time factors for the recovery of transformants, labor intensive protocols, and limitations in regenerating plants from protoplast, callus and suspension cultures. It would be advantageous therefore, to develop efficient transformation methodology which would allow recovery of transgenic plants without the above constraints. Introduction Development of procedures in cell biology to regenerate plants from single cells and organized tissue, and the discovery of novel techniques to transfer genes to plant cells provided the prerequisite for the practical use of genetic engineering in crop improvement. These advances have given us the opportunity to create, characterize and select plant cultivars which could not be obtained by traditional breeding methods. Genetic engineeringof such recalcitrant crops as maize (Fromm et a/., 1990; GordonKamm eta/.,1990), rice (Christou eta/.,1991; Datta eta/., 1990; Toriyama eta/.,1988), cotton (McCabe and Martinell, 1991; Umbeck et a/., 1987), and soybean (Christou et a/., 1990; McCabe et a/., 1988) is now

Journal

The Plant Journal – Wiley

Published: May 1, 1992

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