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Anders Björklund, S. Dunnett, S. Dunnett, Ulf Stenevi, M. Lewis, M. Lewis, Susan Iversen, Susan Iversen (1980)
Reinnervation of the denervated striatum by substantia nigra transplants: Functional consequences as revealed by pharmacological and sensorimotor testingBrain Research, 199
A. Prochiantz, U. Porzio, A. Kato, B. Berger, J. Glowinski (1979)
In vitro maturation of mesencephalic dopaminergic neurons from mouse embryos is enhanced in presence of their striatal target cells.Proceedings of the National Academy of Sciences of the United States of America, 76 10
I. Lorén, A. Björklund, B. Falck, O. Lindvall (1980)
The aluminum-formaldehyde (ALFA) histofluorescence method for improved visualization of catecholamines and indoleamines. 1. A detailed account of methodology for central nervous tissue using paraffin, cryostat or vibratome sectionsJournal of Neuroscience Methods, 2
M. Perlow, W. Freed, B. Hoffer, A. Seiger, L. Olson, R. Wyatt (1979)
Brain grafts reduce motor abnormalities produced by destruction of nigrostriatal dopamine system.Science, 204 4393
Gerald Fischbach (1972)
Synapse formation between dissociated nerve and muscle cells in low density cell cultures.Developmental biology, 28 2
G. Banker, W. Cowan (1977)
Rat hippocampal neurons in dispersed cell cultureBrain Research, 126
U. Stenevi, A. Björklund (1978)
Transplantation techniques for the study of regeneration in the central nervous system.Progress in brain research, 48
D. Gash, J. Sladek (1980)
Vasopressin neurons grafted into Brattleboro rats: Viability and activityPeptides, 1
U. Stenevi, A. Bjo¨rklund, N. Svendgaard (1976)
Transplantation of central and peripheral monoamine neurons to the adult rat brain: Techniques and conditions for survivalBrain Research, 114
(1980)
The aluminum-formaldehyde (ALFA) histogluores
A. Bjo¨rklund, U. Stenevi (1979)
Reconstruction of the nigrostriatal dopamine pathway by intracerebral nigral transplantsBrain Research, 177
J. Marshall, U. Ungerstedt (1977)
Striatal efferent fibers play a role in maintaining rotational behavior in the ratScience, 198
U. Ungerstedt, G. Arbuthnott (1970)
Quantitative recording of rotational behavior in rats after 6-hydroxy-dopamine lesions of the nigrostriatal dopamine system.Brain research, 24 3
Dissociated cell suspensions were prepared from the substantia nigra of 15–17 day-old rat embryos and grafted via an intraparenchymal injection into the depth of the neostriatum of adult recipient rats. The survival and fibre outgrowth of the dopamine-containing neurones in the implants were studied by fluorescence histochemistry, and the functional capacity of the grafts was monitored by repeated testing of the amphetamine-induced turning behaviour of the implanted rats. Before transplantation the target neostriatum of the recipient rats was denervated of its normal dopaminergic innervation by an injection of 6-hydroxydopamine into the ipsilateral nigrostriatal dopamine pathway. The completeness of the denervation was ascertained by measurement of the intensity of the amphetamine-induced turning response. After injection of the dissociated cells large numbers of dopamine-containing neurones were found in clusters at the site of injection as well as scattered in the apparently intact neostriatal tissue up to a distance of about 0.5 mm from the site of injection. Extensive dopamine-containing fibre networks had developed around the implant. These newly formed fibres, which were most abundant around the cell clusters at the injection site, extended in a loose network into large areas of the initially denervated caudate-putamen. In all animals with surviving dopamine neurones the amphetamine-induced turning response was reduced, and in the most extensively reinnervated cases even reversed, within 3–5 weeks after transplantation. This strongly suggests that the implanted dopamine neurones are capable of restoring dopaminergic neurotransmission in the denervated neostriatum, probably via reinnervation of the denervated neostriatal tissue. The use of dissociated brain tissue preparations thus permits reliable intraparenchymal grafting of neurones to plausibly any desired site within the central nervous system, and should open entirely new possibilities for investigation of neuronal growth dynamics and functional reconstruction of damaged brain circuits, perhaps even in brains of larger mammals.
Cell and Tissue Research – Springer Journals
Published: Nov 1, 1980
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