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M. Baucher, B. Chabbert, C. Pilate, J. Doorsselaere, Marie-ThCrPse Tollier, -. MichelPetit, Conil, D. Cornu, B. Monties, M. Montagu, Dirk InzC, L. Jouanin, W. Boerjan (1996)
Red Xylem and Higher Lignin Extractability by Down-Regulating a Cinnamyl Alcohol Dehydrogenase in Poplar, 112
C. Lapierre, B. Pollet, C. Rolando (1995)
New insights into the molecular architecture of hardwood lignins by chemical degradative methodsResearch on Chemical Intermediates, 21
J. Leplé, Rebecca Dauwe, Kris Morreel, V. Storme, C. Lapierre, B. Pollet, A. Naumann, K. Kang, Hoon Kim, K. Ruel, A. Lefèbvre, J. Joseleau, J. Grima-Pettenati, R. Rycke, Sara Andersson-Gunnerås, A. Erban, I. Fehrle, M. Petit-Conil, J. Kopka, A. Polle, E. Messens, B. Sundberg, S. Mansfield, J. Ralph, G. Pilate, W. Boerjan (2007)
Downregulation of Cinnamoyl-Coenzyme A Reductase in Poplar: Multiple-Level Phenotyping Reveals Effects on Cell Wall Polymer Metabolism and Structure[W]The Plant Cell Online, 19
K. Vogel, Jeffrey Pedersen, S. Masterson, J. Toy (1999)
Evaluation of a Filter Bag System for NDF, ADF, and IVDMD Forage AnalysisCrop Science, 39
C. Lapierre, G. Pilate, B. Pollet, I. Mila, J. Leplé, L. Jouanin, Hoon Kim, J. Ralph (2004)
Signatures of cinnamyl alcohol dehydrogenase deficiency in poplar lignins.Phytochemistry, 65 3
(2006)
Journal of Experimental Botany Advance Access published March 21, 2006 Journal of Experimental Botany, Page 1 of 13
M. Ashburner (1989)
A Laboratory manual
Matthieu Chabannes, A. Barakate, Catherine Lapierre, J. Marita, John Ralph, Michel Pean, S. Danoun, C. Halpin, J. Grima-Pettenati, and Boudet (2001)
Strong decrease in lignin content without significant alteration of plant development is induced by simultaneous down-regulation of cinnamoyl CoA reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD) in tobacco plants.The Plant journal : for cell and molecular biology, 28 3
W. Boerjan, J. Ralph, M. Baucher (2003)
Lignin biosynthesis.Annual review of plant biology, 54
Thomas Goujon, Valérie Ferret, I. Mila, B. Pollet, K. Ruel, V. Burlat, J. Joseleau, Y. Barrière, C. Lapierre, L. Jouanin (2003)
Down-regulation of the AtCCR1 gene in Arabidopsis thaliana: effects on phenotype, lignins and cell wall degradabilityPlanta, 217
Stephen Lin, C. Dence (1992)
Methods in Lignin Chemistry
Di Guo, Fang Chen, J. Wheeler, J. Winder, Susan Selman, M. Peterson, R. Dixon (2001)
Improvement of in-rumen digestibility of alfalfa forage by genetic manipulation of lignin O-methyltransferasesTransgenic Research, 10
A. Ragauskas, Charlotte Williams, B. Davison, George Britovsek, J. Cairney, C. Eckert, W. Frederick, J. Hallett, D. Leak, C. Liotta, J. Mielenz, R. Murphy, R. Templer, T. Tschaplinski (2006)
The Path Forward for Biofuels and BiomaterialsScience, 311
H. Wengenmayer, J. Ebel, H. Grisebach (1976)
Enzymic Synthesis of Lignin PrecursorsFEBS Journal, 65
B. Dien, Hansang Jung, K. Vogel, M. Casler, J. Lamb, L. Iten, R. Mitchell, G. Sarath (2006)
Chemical composition and response to dilute-acid pretreatment and enzymatic saccharification of alfalfa, reed canarygrass, and switchgrassBiomass & Bioenergy, 30
Fang Chen, M. Reddy, S. Temple, Lisa Jackson, Gail Shadle, R. Dixon (2006)
Multi-site genetic modulation of monolignol biosynthesis suggests new routes for formation of syringyl lignin and wall-bound ferulic acid in alfalfa (Medicago sativa L.).The Plant journal : for cell and molecular biology, 48 1
A. O'connell, Karen Holt, J. Piquemal, J. Grima-Pettenati, A. Boudet, B. Pollet, C. Lapierre, M. Petit-Conil, W. Schuch, C. Halpin (2002)
Improved Paper Pulp from Plants with Suppressed Cinnamoyl-CoA Reductase or Cinnamyl Alcohol DehydrogenaseTransgenic Research, 11
B. Davison, S. Drescher, G. Tuskan, Mark Davis, N. Nghiem (2006)
Variation of S/G ratio and lignin content in a Populus family influences the release of xylose by dilute acid hydrolysisApplied Biochemistry and Biotechnology, 130
C. Halpin, M. Knight, G. Foxon, M. Campbell, A. Boudet, J. Boon, B. Chabbert, M. Tollier, W. Schuch (1994)
Manipulation of lignin quality by downregulation of cinnamyl alcohol dehydrogenasePlant Journal, 6
C. Luo, D. Brink, H. Blanch (2002)
Identification of potential fermentation inhibitors in conversion of hybrid poplar hydrolyzate to ethanolBiomass & Bioenergy, 22
E. Kellogg (2001)
Medicago truncatula, going where no plant has gone before
Cao-Trung Do, B. Pollet, J. Thévenin, Richard Sibout, D. Denoue, Y. Barrière, C. Lapierre, L. Jouanin (2007)
Both caffeoyl Coenzyme A 3-O-methyltransferase 1 and caffeic acid O-methyltransferase 1 are involved in redundant functions for lignin, flavonoids and sinapoyl malate biosynthesis in ArabidopsisPlanta, 226
A. Boudet, S. Kajita, J. Grima-Pettenati, D. Goffner (2003)
Lignins and lignocellulosics: a better control of synthesis for new and improved uses.Trends in plant science, 8 12
(2007)
Alfalfa’s bioenergy appeal
B Rest, S Danoun, AM Boudet (2006)
Down-regulation of cinnamoyl-CoA reductase in tomato (Solanum lycopersicum L.) induces dramatic changes in soluble phenolic poolsJ Exp Bot, 57
Gail Shadle, Fang Chen, M. Reddy, Lisa Jackson, J. Nakashima, R. Dixon (2007)
Down-regulation of hydroxycinnamoyl CoA: shikimate hydroxycinnamoyl transferase in transgenic alfalfa affects lignification, development and forage quality.Phytochemistry, 68 11
J. Hein (1990)
Unified approach to alignment and phylogenies.Methods in enzymology, 183
L. Jones, A. Ennos, S. Turner (2001)
Cloning and characterization of irregular xylem4 (irx4): a severely lignin-deficient mutant of Arabidopsis.The Plant journal : for cell and molecular biology, 26 2
Lei Chen, C. Auh, P. Dowling, J. Bell, Fang Chen, A. Hopkins, R. Dixon, Zeng‐Yu Wang (2003)
Improved forage digestibility of tall fescue (Festuca arundinacea) by transgenic down-regulation of cinnamyl alcohol dehydrogenase.Plant biotechnology journal, 1 6
T. Hibino, K. Takabe, T. Kawazu, D. Shibata, T. Higuchi (1995)
Increase of Cinnamaldehyde Groups in Lignin of Transgenic Tobacco Plants Carrying an Antisense Gene for Cinnamyl Alcohol DehydrogenaseBioscience, Biotechnology, and Biochemistry, 59
D. Wyrambik, H. Grisebach (1979)
Enzymic synthesis of lignin precursors. Further studies on cinnamyl-alcohol dehydrogenase from soybean-cell-suspension cultures.European journal of biochemistry, 97 2
K. Parvathi, Fang Chen, D. Guo, J. Blount, R. Dixon (2008)
Substrate preferences of O-methyltransferases in alfalfa suggest new pathways for 3-O-methylation of monolignols.The Plant journal : for cell and molecular biology, 25 2
J. Sambrook, E. Fritsch, T. Maniatis (2001)
Molecular Cloning: A Laboratory Manual
Sébastien Besseau, Laurent Hoffmann, P. Geoffroy, C. Lapierre, B. Pollet, M. Legrand (2007)
Flavonoid Accumulation in Arabidopsis Repressed in Lignin Synthesis Affects Auxin Transport and Plant GrowthThe Plant Cell Online, 19
M. Chabannes, K. Ruel, A. Yoshinaga, B. Chabbert, A. Jauneau, J. Joseleau, A. Boudet (2001)
In situ analysis of lignins in transgenic tobacco reveals a differential impact of individual transformations on the spatial patterns of lignin deposition at the cellular and subcellular levels.The Plant journal : for cell and molecular biology, 28 3
John Ralph, Ronald Hatfield, J. Piquemal, N. Yahiaoui, Michel Pean, Catherine Lapierre, A. Boudet (1998)
NMR characterization of altered lignins extracted from tobacco plants down-regulated for lignification enzymes cinnamylalcohol dehydrogenase and cinnamoyl-CoA reductase.Proceedings of the National Academy of Sciences of the United States of America, 95 22
Richard Sibout, A. Eudes, G. Mouille, B. Pollet, C. Lapierre, L. Jouanin, A. Séguin (2005)
CINNAMYL ALCOHOL DEHYDROGENASE-C and -D Are the Primary Genes Involved in Lignin Biosynthesis in the Floral Stem of Arabidopsisw⃞The Plant Cell Online, 17
Marie Vailhé, J. Besle, M. Maillot, A. Cornu, C. Halpin, M. Knight (1998)
Effect of down-regulation of cinnamyl alcohol dehydrogenase on cell wall composition and on degradability of tobacco stemsJournal of the Science of Food and Agriculture, 76
K. Vogel, Hansang Jung (2001)
Genetic Modification of Herbaceous Plants for Feed and FuelCritical Reviews in Plant Sciences, 20
(1992)
Thioacidolysis. In: Lin SY, Dence CW (eds) Methods in lignin chemistry
G. Pilate, E. Guiney, Karen Holt, M. Petit-Conil, C. Lapierre, J. Leplé, B. Pollet, I. Mila, E. Webster, H. Marstorp, D. Hopkins, L. Jouanin, W. Boerjan, W. Schuch, D. Cornu, C. Halpin (2002)
Field and pulping performances of transgenic trees with altered lignificationNature Biotechnology, 20
J. Nakashima, Fang Chen, Lisa Jackson, Gail Shadle, R. Dixon (2008)
Multi-site genetic modification of monolignol biosynthesis in alfalfa (Medicago sativa): effects on lignin composition in specific cell types.The New phytologist, 179 3
D. Guo, Fang Chen, K. Inoue, J. Blount, R. Dixon (2001)
Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa: Impacts on Lignin Structure and Implications for the Biosynthesis of G and S LigninPlant Cell, 13
J. Lamb, C. Sheaffer, D. Samac (2003)
Population Density and Harvest Maturity Effects on Leaf and Stem Yield in AlfalfaAgronomy Journal, 95
M. Bradford (1976)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical biochemistry, 72
J. Humphreys, C. Chapple (2002)
Rewriting the lignin roadmap.Current opinion in plant biology, 5 3
C. Lapierre, B. Pollet, J. MacKay, R. Sederoff (2000)
Lignin structure in a mutant pine deficient in cinnamyl alcohol dehydrogenase.Journal of agricultural and food chemistry, 48 6
Laurent Hoffmann, S. Maury, F. Martz, P. Geoffroy, M. Legrand (2003)
Purification, Cloning, and Properties of an Acyltransferase Controlling Shikimate and Quinate Ester Intermediates in Phenylpropanoid Metabolism*The Journal of Biological Chemistry, 278
C. Lapierre, B. Pollet, M. Petit-Conil, G. Toval, J. Romero, G. Pilate, J. Leplé, W. Boerjan, Valérie Ferret, V. Nadai, L. Jouanin (1999)
Structural alterations of lignins in transgenic poplars with depressed cinnamyl alcohol dehydrogenase or caffeic acid O-methyltransferase activity have an opposite impact on the efficiency of industrial kraft pulpingPlant physiology, 119 1
M. Reddy, Fang Chen, Gail Shadle, Lisa Jackson, H. Aljoe, R. Dixon (2005)
Targeted down-regulation of cytochrome P450 enzymes for forage quality improvement in alfalfa (Medicago sativa L.).Proceedings of the National Academy of Sciences of the United States of America, 102 46
J. Piquemal, C. Lapierre, K. Myton, A. O'connell, W. Schuch, J. Grima-Pettenati, A. Boudet (2002)
Down-regulation of Cinnamoyl-CoA reductase induces significant changes of lignin profiles in transgenic tobacco plantsPlant Journal, 13
C. Lapierre, B. Monties, C. Rolando, Laboratorie Chirale (1985)
Thioacidolysis of Lignin: Comparison with AcidolysisJournal of Wood Chemistry and Technology, 5
(2006)
Variation of S/ G ratio and lignin content in a Populus family influences the release of fermentable sugars by dilute acid hydrolysis
A. Eudes, B. Pollet, Richard Sibout, Cao-Trung Do, A. Séguin, C. Lapierre, L. Jouanin (2006)
Evidence for a role of AtCAD 1 in lignification of elongating stems of Arabidopsis thalianaPlanta, 225
M. Baucher, M. Bernard-vailhé, B. Chabbert, J. Besle, C. Opsomer, M. Montagu, J. Botterman (1999)
Down-regulation of cinnamyl alcohol dehydrogenase in transgenic alfalfa (Medicago sativa L.) and the effect on lignin composition and digestibilityPlant Molecular Biology, 39
M. Dubois, K. Gilles, J. Hamilton, P. Rebers, F. Smith (1956)
Colorimetric Method for Determination of Sugars and Related SubstancesAnalytical Chemistry, 28
M. Reddy, S. Ghabrial, C. Redmond, R. Dinkins, G. Collins (2001)
Resistance to Bean pod mottle virus in Transgenic Soybean Lines Expressing the Capsid Polyprotein.Phytopathology, 91 9
Fang Chen, R. Dixon (2007)
Lignin modification improves fermentable sugar yields for biofuel productionNature Biotechnology, 25
A series of transgenic lines of alfalfa (Medicago sativa) were generated in which either one of the two potentially terminal enzymes of the monolignol pathway, cinnamoyl CoA reductase (CCR) or cinnamyl alcohol dehydrogenase (CAD) was down-regulated by expression of antisense transgenes. Levels of CCR enzymatic activity were reduced to between 10% to 65% of the control level, and levels of CAD activity were similarly reduced to between 5% to 40% of the control. Biomass yields were reduced in the most strongly down-regulated lines for both transgenes, but many of the lines exhibited reduced lignin levels but normal biomass and flowering time. In vitro dry matter digestibility was increased for most transgenic lines compared to controls. Saccharification efficiency was determined by measuring the release of sugars from cell walls directly, or after sulfuric acid pre-treatment and subsequent digestion with a mixture of cellulase and cellobiase. Several CCR down-regulated lines had significantly enhanced saccharification efficiency with both pre-treated and untreated tissues, whereas CAD down-regulation had less impact on sugar release when compared to that from CCR lines with similar lignin contents. One CCR line with a 50–60% improvement in saccharification efficiency exhibited normal biomass production, indicating the potential for producing high yielding, improved feedstocks for bioethanol production through genetic modification of the monolignol pathway.
BioEnergy Research – Springer Journals
Published: Sep 27, 2008
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