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Effect of zinc on gene expression in osteoblastic MC3T3-E1 cells: enhancement of Runx2, OPG, and regucalcin mRNA expressions

Effect of zinc on gene expression in osteoblastic MC3T3-E1 cells: enhancement of Runx2, OPG, and... The effect of zinc sulfate on the mRNA expressions in Runx2, osteocalcin, α1(I) collagen, insulin-like growth factor-I (IGF-I), transforming growth factor-β1 (TGF-β1), osteoprotegerin (OPG), regucalcin, zinc transporter 1 (ZIP1), or glyceroaldehyde-3-phosphate dehydrogenase (G3PDH) in osteoblastic MC3T3-E1 cells in vitro was investigated. Cells with subconfluency were cultured for 48 h in a medium containing either vehicle or zinc sulfate (10−6–10−4 M) without fetal bovine serum. Culture with zinc sulfate (10−5 M) caused a significant increase in Runx2, OPG, or regucalcin mRNA expressions in the cells, while it did not have a significant effect on osteocalcin, α1(I) collagen, IGF-I, TGF-β1, ZIP1, or G3PDH mRNA expressions. The effect of zinc sulfate (10−4 M) in increasing Runx2 mRNA expression was seen at 24–72 h after culture. A significant increase in OPG mRNA expression was observed at 24 or 48 h after culture. Regucalcin mRNA expression was significantly increased at 48 or 72 h after culture with zinc sulfate (10−4 M). The stimulatory effects of zinc sulfate on Runx2, OPG, or regucalcin mRNAs were significantly prevented in the presence of cycloheximide (10−7 M), an inhibitor of protein synthesis, or 5,6-dichloro-1-β-d-ribofuranosyl-benzimidazole (10−6 M), an inhibitor of transcription activity. Culture with β-alanyl-l-histidinato zinc (10−5 M) caused a significant increase in Runx2 or regucalcin mRNA expressions, while zinc acexamate (10−5 M) did not have a significant effect on Runx2, OPG, ZIP1, or regucalcin mRNA expressions. This study demonstrates that zinc sulfate has a role in the enhancement of Runx2, OPG, or regucalcin mRNA expression in osteoblastic cells in vitro, suggesting its role in the regulation of gene expression in the cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular and Cellular Biochemistry Springer Journals

Effect of zinc on gene expression in osteoblastic MC3T3-E1 cells: enhancement of Runx2, OPG, and regucalcin mRNA expressions

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References (47)

Publisher
Springer Journals
Copyright
Copyright © 2008 by Springer Science+Business Media, LLC.
Subject
Life Sciences; Cardiology; Oncology ; Medical Biochemistry; Biochemistry, general
ISSN
0300-8177
eISSN
1573-4919
DOI
10.1007/s11010-008-9731-7
pmid
18327666
Publisher site
See Article on Publisher Site

Abstract

The effect of zinc sulfate on the mRNA expressions in Runx2, osteocalcin, α1(I) collagen, insulin-like growth factor-I (IGF-I), transforming growth factor-β1 (TGF-β1), osteoprotegerin (OPG), regucalcin, zinc transporter 1 (ZIP1), or glyceroaldehyde-3-phosphate dehydrogenase (G3PDH) in osteoblastic MC3T3-E1 cells in vitro was investigated. Cells with subconfluency were cultured for 48 h in a medium containing either vehicle or zinc sulfate (10−6–10−4 M) without fetal bovine serum. Culture with zinc sulfate (10−5 M) caused a significant increase in Runx2, OPG, or regucalcin mRNA expressions in the cells, while it did not have a significant effect on osteocalcin, α1(I) collagen, IGF-I, TGF-β1, ZIP1, or G3PDH mRNA expressions. The effect of zinc sulfate (10−4 M) in increasing Runx2 mRNA expression was seen at 24–72 h after culture. A significant increase in OPG mRNA expression was observed at 24 or 48 h after culture. Regucalcin mRNA expression was significantly increased at 48 or 72 h after culture with zinc sulfate (10−4 M). The stimulatory effects of zinc sulfate on Runx2, OPG, or regucalcin mRNAs were significantly prevented in the presence of cycloheximide (10−7 M), an inhibitor of protein synthesis, or 5,6-dichloro-1-β-d-ribofuranosyl-benzimidazole (10−6 M), an inhibitor of transcription activity. Culture with β-alanyl-l-histidinato zinc (10−5 M) caused a significant increase in Runx2 or regucalcin mRNA expressions, while zinc acexamate (10−5 M) did not have a significant effect on Runx2, OPG, ZIP1, or regucalcin mRNA expressions. This study demonstrates that zinc sulfate has a role in the enhancement of Runx2, OPG, or regucalcin mRNA expression in osteoblastic cells in vitro, suggesting its role in the regulation of gene expression in the cells.

Journal

Molecular and Cellular Biochemistry – Springer Journals

Published: Mar 9, 2008

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