Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Expression pattern of two paralogs of the PI / GLO -like locus during Orchis italica (Orchidaceae, Orchidinae) flower development

Expression pattern of two paralogs of the PI / GLO -like locus during Orchis italica... The class B MADS-box genes belong to two distinct functional groups: the AP3/DEF -like and the PI/GLO -like sub-families. In orchids, AP3/DEF -like genes are present in four copies, each with a different role in floral organ formation, which is described in the “orchid code” model. Interestingly, the orchid PI/GLO -like genes are present in two copies in Orchidinae, whereas they are described as single copy in the other orchid lineages. The two PI/GLO -like paralogs have site-specific different selective constraints; in addition, they show relaxation of purifying selection when compared to the single-copy lineages. In this study, we present a comparative analysis of the expression patterns of the two PI/GLO -like paralogs, OrcPI and OrcPI2 , in floral tissues of Orchis italica in different developmental stages using real-time PCR. The two genes show similar expression profiles in the tissue examined, with differences detectable between immature and mature inflorescence. In all cases, OrcPI2 is expressed at a higher level than OrcPI . Real-time PCR results reveal that the co-expression of the two duplicated loci could have a fully or partially redundant function. The possible evolutionary fate of OrcPI and OrcPI2 is discussed as well as their involvement in ovary development. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Development Genes and Evolution Springer Journals

Expression pattern of two paralogs of the PI / GLO -like locus during Orchis italica (Orchidaceae, Orchidinae) flower development

Loading next page...
 
/lp/springer-journals/expression-pattern-of-two-paralogs-of-the-pi-glo-like-locus-during-iz8i9tT8cc

References (16)

Publisher
Springer Journals
Copyright
Copyright © 2011 by Springer-Verlag
Subject
Life Sciences; Animal Genetics and Genomics; Biochemistry, general; Developmental Biology; Cell Biology; Neurosciences
ISSN
0949-944X
eISSN
1432-041X
DOI
10.1007/s00427-011-0372-6
pmid
21892737
Publisher site
See Article on Publisher Site

Abstract

The class B MADS-box genes belong to two distinct functional groups: the AP3/DEF -like and the PI/GLO -like sub-families. In orchids, AP3/DEF -like genes are present in four copies, each with a different role in floral organ formation, which is described in the “orchid code” model. Interestingly, the orchid PI/GLO -like genes are present in two copies in Orchidinae, whereas they are described as single copy in the other orchid lineages. The two PI/GLO -like paralogs have site-specific different selective constraints; in addition, they show relaxation of purifying selection when compared to the single-copy lineages. In this study, we present a comparative analysis of the expression patterns of the two PI/GLO -like paralogs, OrcPI and OrcPI2 , in floral tissues of Orchis italica in different developmental stages using real-time PCR. The two genes show similar expression profiles in the tissue examined, with differences detectable between immature and mature inflorescence. In all cases, OrcPI2 is expressed at a higher level than OrcPI . Real-time PCR results reveal that the co-expression of the two duplicated loci could have a fully or partially redundant function. The possible evolutionary fate of OrcPI and OrcPI2 is discussed as well as their involvement in ovary development.

Journal

Development Genes and EvolutionSpringer Journals

Published: Oct 1, 2011

There are no references for this article.