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Peptidase D (prolidase) variants in man

Peptidase D (prolidase) variants in man Peptidase D (prolidase) variants in man BY W. H. P. LEWIS* AND HARRY HARRIS Medical Research Council Human Biochemical Genetics Unit, The Galton Laboratory, University College London A method for the detection of peptidases after electrophoresis on starch gel was described by Lewis & Harris (1967) and applied to the study of red cells obtained from different individuals. Five different peptidases, which could be distinguished in terms of their electrophoretic properties and their patterns of substrate specificity were found to occur, and were designated peptidases A, B, C, D and E. I n the course of population surveys a number of inherited variants of peptidase A and also peptidase B were discovered (Lewis & Harris, 1967, and Lewis,Corney & Harris, 1968). In the present paper we describe some variants of peptidam D. METHODS Red blood cells were prepared by washing twice in 0.9 % NaCl and lysed by freezing and thawing. Mercaptoethanol was added to the lysates to give a concentration of approximately 20 mM. Starch gel electrophoresis and the detection of peptidase activity was carried out as described by Lewis & Harris (1967), with the exception that the concentration of the gel buffer was 0.01 molar. This http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Annals of Human Genetics Wiley

Peptidase D (prolidase) variants in man

LEWIS, W. H. P.; HARRIS, HARRY
Annals of Human Genetics , Volume 32 (4) – May 1, 1969
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References (4)

Publisher
Wiley
Copyright
Copyright © 1969 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0003-4800
eISSN
1469-1809
DOI
10.1111/j.1469-1809.1969.tb00081.x
Publisher site
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Abstract

Peptidase D (prolidase) variants in man BY W. H. P. LEWIS* AND HARRY HARRIS Medical Research Council Human Biochemical Genetics Unit, The Galton Laboratory, University College London A method for the detection of peptidases after electrophoresis on starch gel was described by Lewis & Harris (1967) and applied to the study of red cells obtained from different individuals. Five different peptidases, which could be distinguished in terms of their electrophoretic properties and their patterns of substrate specificity were found to occur, and were designated peptidases A, B, C, D and E. I n the course of population surveys a number of inherited variants of peptidase A and also peptidase B were discovered (Lewis & Harris, 1967, and Lewis,Corney & Harris, 1968). In the present paper we describe some variants of peptidam D. METHODS Red blood cells were prepared by washing twice in 0.9 % NaCl and lysed by freezing and thawing. Mercaptoethanol was added to the lysates to give a concentration of approximately 20 mM. Starch gel electrophoresis and the detection of peptidase activity was carried out as described by Lewis & Harris (1967), with the exception that the concentration of the gel buffer was 0.01 molar. This

Journal

Annals of Human Genetics – Wiley

Published: May 1, 1969

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