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Evidence that anandamide and EDHF act via different mechanisms in rat isolated mesenteric arteries

Evidence that anandamide and EDHF act via different mechanisms in rat isolated mesenteric arteries The endogenous cannabinoid, anandamide, has been suggested as an endothelium‐derived hyperpolarizing factor (EDHF). We found that anandamide‐evoked relaxation in isolated segments of rat mesenteric artery was associated with smooth muscle hyperpolarization. However, although anandamide‐evoked relaxation was inhibited by either charybdotoxin (ChTX) or iberiotoxin, inhibition of the relaxation to EDHF required a combination of ChTX and apamin. The relaxations induced by either anandamide or EDHF were not inhibited by the cannabinoid receptor (CB1) antagonist SRI41716A, or mimicked by selective CB1 agonists. Thus, anandamide appears to cause smooth muscle relaxation via a CB1 receptor‐independent mechanism and cannabinoid receptor activation apparently does not contribute to EDHF‐mediated relaxation in this resistance artery. British Journal of Pharmacology (1997) 121, 1509–1511; doi:10.1038/sj.bjp.0701361 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png British Journal of Pharmacology Wiley

Evidence that anandamide and EDHF act via different mechanisms in rat isolated mesenteric arteries

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References (8)

Publisher
Wiley
Copyright
1997 British Pharmacological Society
ISSN
0007-1188
eISSN
1476-5381
DOI
10.1038/sj.bjp.0701361
pmid
9283682
Publisher site
See Article on Publisher Site

Abstract

The endogenous cannabinoid, anandamide, has been suggested as an endothelium‐derived hyperpolarizing factor (EDHF). We found that anandamide‐evoked relaxation in isolated segments of rat mesenteric artery was associated with smooth muscle hyperpolarization. However, although anandamide‐evoked relaxation was inhibited by either charybdotoxin (ChTX) or iberiotoxin, inhibition of the relaxation to EDHF required a combination of ChTX and apamin. The relaxations induced by either anandamide or EDHF were not inhibited by the cannabinoid receptor (CB1) antagonist SRI41716A, or mimicked by selective CB1 agonists. Thus, anandamide appears to cause smooth muscle relaxation via a CB1 receptor‐independent mechanism and cannabinoid receptor activation apparently does not contribute to EDHF‐mediated relaxation in this resistance artery. British Journal of Pharmacology (1997) 121, 1509–1511; doi:10.1038/sj.bjp.0701361

Journal

British Journal of PharmacologyWiley

Published: Aug 1, 1997

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