Access the full text.
Sign up today, get DeepDyve free for 14 days.
R Amasino (2010)
Seasonal and developmental timing of floweringPlant J, 61
A. Samach, H. Onouchi, S. Gold, G. Ditta, Z. Schwarz‐Sommer, M. Yanofsky, G. Coupland (2000)
Distinct roles of CONSTANS target genes in reproductive development of Arabidopsis.Science, 288 5471
Chengying Shi, Hua Yang, Chaoling Wei, O. Yu, Zheng-Zhu Zhang, Changjun Jiang, Jun Sun, Yeyun Li, Qi Chen, Tao Xia, X. Wan (2011)
Deep sequencing of the Camellia sinensis transcriptome revealed candidate genes for major metabolic pathways of tea-specific compoundsBMC Genomics, 12
Ryosuke Hayama, G. Coupland (2003)
Shedding light on the circadian clock and the photoperiodic control of flowering.Current opinion in plant biology, 6 1
C. Iseli, C. Jongeneel, P. Bucher (1999)
ESTScan: A Program for Detecting, Evaluating, and Reconstructing Potential Coding Regions in EST SequencesProceedings. International Conference on Intelligent Systems for Molecular Biology
M. Metzker (2010)
Sequencing technologies — the next generationNature Reviews Genetics, 11
F. Valverde (2011)
CONSTANS and the evolutionary origin of photoperiodic timing of flowering.Journal of experimental botany, 62 8
P. Suárez-López, Kay Wheatley, F. Robson, H. Onouchi, F. Valverde, G. Coupland (2001)
CONSTANS mediates between the circadian clock and the control of flowering in ArabidopsisNature, 410
M. Bouzidi, Jérôme Franchel, Q. Tao, K. Stormo, A. Mraz, P. Nicolas, S. Mouzeyar (2006)
A sunflower BAC library suitable for PCR screening and physical mapping of targeted genomic regionsTheoretical and Applied Genetics, 113
M. Harris, Jennifer Clark, A. Ireland, J. Lomax, M. Ashburner, M. Ashburner, R. Foulger, R. Foulger, K. Eilbeck, K. Eilbeck, S. Lewis, S. Lewis, B. Marshall, B. Marshall, C. Mungall, C. Mungall, J. Richter, J. Richter, G. Rubin, Gerald Rubin, J. Blake, C. Bult, M. Dolan, H. Drabkin, J. Eppig, Hill Dp, L. Ni, M. Ringwald, R. Balakrishnan, R. Balakrishnan, J. Cherry, J. Cherry, K. Christie, K. Christie, M. Costanzo, M. Costanzo, S. Dwight, S. Dwight, S. Engel, S. Engel, D. Fisk, D. Fisk, J. Hirschman, J. Hirschman, Eurie Hong, Eurie Hong, R. Nash, R. Nash, A. Sethuraman, A. Sethuraman, C. Theesfeld, C. Theesfeld, D. Botstein, D. Botstein, K. Dolinski, K. Dolinski, B. Feierbach, B. Feierbach, T. Berardini, T. Berardini, S. Mundodi, S. Mundodi, S. Rhee, S. Rhee, R. Apweiler, D. Barrell, E. Camon, E. Dimmer, Lee, R. Chisholm, P. Gaudet, P. Gaudet, W. Kibbe, W. Kibbe, R. Kishore, R. Kishore, E. Schwarz, E. Schwarz, P. Sternberg, P. Sternberg, M. Gwinn, L. Hannick, J. Wortman, M. Berriman, Wood, N. Delacruz, N. Delacruz, P. Tonellato, P. Tonellato, P. Jaiswal, P. Jaiswal, T. Seigfried, T. Seigfried, R. White, R. White (2004)
Gene Ontology Consortium: The Gene Ontology (GO) database and informatics resourceNucleic acids research, 32 Database issue
K. Borden (1998)
RING fingers and B-boxes: zinc-binding protein-protein interaction domains.Biochemistry and cell biology = Biochimie et biologie cellulaire, 76 2-3
Cheng-Jing Hou, Chang-Hsien Yang (2009)
Functional analysis of FT and TFL1 orthologs from orchid (Oncidium Gower Ramsey) that regulate the vegetative to reproductive transition.Plant & cell physiology, 50 8
Gene Consortium (2003)
The Gene Ontology (GO) database and informatics resource
C. Bishop (2003)
Control of FloweringGenome Biology, 4
F. Robson, M. Costa, S. Hepworth, I. Vizir, M. Piñeiro, P. Reeves, J. Putterill, G. Coupland (2002)
Functional importance of conserved domains in the flowering-time gene CONSTANS demonstrated by analysis of mutant alleles and transgenic plants.The Plant journal : for cell and molecular biology, 28 6
S. Altschul, W. Gish, W. Miller, E. Myers, D. Lipman (1990)
Basic local alignment search tool.Journal of molecular biology, 215 3
W Garner, HA Allard (1920)
Effect of the relative length of day and night and other factors of the environment on growth and reproduction in plantsJ Agricultural Res, 18
M. Koornneef, C. Hanhart, J. Veen (1991)
A genetic and physiological analysis of late flowering mutants in Arabidopsis thalianaMolecular and General Genetics MGG, 229
T. Murashige, F. Skoog (1962)
A revised medium for rapid growth and bio assays with tobacco tissue culturesPhysiologia Plantarum, 15
Chih-Hsiung Fu, Y. Chen, Yu-Yun Hsiao, Zhao-Jun Pan, Zhongjian Liu, Yueh-Min Huang, W. Tsai, Hong-Hwa Chen (2011)
OrchidBase: a collection of sequences of the transcriptome derived from orchids.Plant & cell physiology, 52 2
R. Tatusov, D. Natale, I. Garkavtsev, T. Tatusova, U. Shankavaram, B. Rao, B. Kiryutin, Michael Galperin, N. Fedorova, E. Koonin (2001)
The COG database: new developments in phylogenetic classification of proteins from complete genomesNucleic acids research, 29 1
Hsing-Fun Hsu, Wen-Ping Hsieh, Ming-Kun Chen, Yu-Yun Chang, Chang-Hsien Yang (2010)
C/D class MADS box genes from two monocots, orchid (Oncidium Gower Ramsey) and lily (Lilium longiflorum), exhibit different effects on floral transition and formation in Arabidopsis thaliana.Plant & cell physiology, 51 6
(2011)
In vitro fruting and seed production in Erycina pusilla ( L . ) N . H . Willams and M . W . Chase
Maureen Torok, L. Etkin (2001)
Two B or not two B? Overview of the rapidly expanding B-box family of proteins.Differentiation; research in biological diversity, 67 3
A. Russell, R. Samuel, Verena Klejna, Michael Barfuss, Barbara Rupp, M. Chase (2010)
Reticulate evolution in diploid and tetraploid species of Polystachya (Orchidaceae) as shown by plastid DNA sequences and low-copy nuclear genes.Annals of botany, 106 1
Jia Ye, L. Fang, Hongkun Zheng, Yong Zhang, Jing Chen, Zengjin Zhang, Jing Wang, Shengting Li, Ruiqiang Li, L. Bolund, Jun Wang (2006)
WEGO: a web tool for plotting GO annotationsNucleic Acids Research, 34
Hsing-Fun Hsu, Chang-Hsien Yang (2002)
An orchid (Oncidium Gower Ramsey) AP3-like MADS gene regulates floral formation and initiation.Plant & cell physiology, 43 10
Da-Cheng Hao, Pei Ma, J. Mu, Shi-lin Chen, P. Xiao, Yong Peng, Liping Huo, Lijia Xu, Chao Sun (2012)
De novo characterization of the root transcriptome of a traditional Chinese medicinal plant Polygonum cuspidatumScience China Life Sciences, 55
S. Datta, G. Hettiarachchi, X. Deng, M. Holm (2005)
Arabidopsis CONSTANS-LIKE3 Is a Positive Regulator of Red Light Signaling and Root Growth[W]The Plant Cell Online, 18
S. Griffiths, R. Dunford, G. Coupland, D. Laurie (2003)
The Evolution of CONSTANS-Like Gene Families in Barley, Rice, and Arabidopsis1Plant Physiology, 131
Rie Kikuchi, H. Kawahigashi, M. Oshima, T. Ando, H. Handa (2011)
The differential expression of HvCO9, a member of the CONSTANS-like gene family, contributes to the control of flowering under short-day conditions in barleyJournal of Experimental Botany, 63
Orna Ben-Naim, Ravit Eshed, Anna Parnis, Paula Teper-Bamnolker, Akiva Shalit, G. Coupland, A. Samach, E. Lifschitz (2006)
The CCAAT binding factor can mediate interactions between CONSTANS-like proteins and DNA.The Plant journal : for cell and molecular biology, 46 3
A Conesa, S Götz, JM García-Gómez, J Terol, M Talón, M Robles (2005)
Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics researchBioinformatics, 21
R. Khanna, B. Kronmiller, D. Maszle, G. Coupland, M. Holm, T. Mizuno, Shu-Hsing Wu (2009)
The Arabidopsis B-Box Zinc Finger Family[W]The Plant Cell Online, 21
A. Mouradov, F. Cremer, G. Coupland (2002)
Control of flowering time: interacting pathways as a basis for diversity.The Plant cell, 14 Suppl
Jiao Chen, Jian-ye Chen, Jun-ning Wang, J. Kuang, W. Shan, Wang-jin Lu (2012)
Molecular characterization and expression profiles of MaCOL1, a CONSTANS-like gene in banana fruit.Gene, 496 2
J. Heslop-Harrison (1964)
The Transition to FloweringNature, 202
R. Amasino (2010)
ARABIDOPSIS : A RICH HARVEST 10 YEARS AFTER COMPLETION OF THE GENOME SEQUENCE Seasonal and developmental timing of flowering
Ruiqiang Li, Hong-mei Zhu, Jue Ruan, W. Qian, X. Fang, Z. Shi, Yingrui Li, Shengting Li, Gao Shan, K. Kristiansen, Songgang Li, Huanming Yang, Jian Wang, Jun Wang (2010)
De novo assembly of human genomes with massively parallel short read sequencing.Genome research, 20 2
R. Hayama, T. Izawa, K. Shimamoto (2002)
Isolation of rice genes possibly involved in the photoperiodic control of flowering by a fluorescent differential display method.Plant & cell physiology, 43 5
I. Pan, De-Chih Liao, Fu-Huei Wu, H. Daniell, N. Singh, Chen Chang, M. Shih, M. Chan, Choun-Sea Lin (2012)
Complete Chloroplast Genome Sequence of an Orchid Model Plant Candidate: Erycina pusilla Apply in Tropical Oncidium BreedingPLoS ONE, 7
R. Ness, M. Siol, S. Barrett (2011)
De novo sequence assembly and characterization of the floral transcriptome in cross- and self-fertilizing plantsBMC Genomics, 12
Chen-Tran Hsu, De-Chih Liao, Fu-Hui Wu, Nien-Tze Liu, Shu-Chen Shen, S. Chou, Shu-Yun Tung, Chang-Hsien Yang, M. Chan, Choun-Sea Lin (2011)
Integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of Oncidium Gower RamseyBMC Plant Biology, 11
E. Mardis (2008)
Next-generation DNA sequencing methods.Annual review of genomics and human genetics, 9
W. Garner, H. Allard (1920)
EFFECT OF THE RELATIVE LENGTH OF DAY AND NIGHT AND OTHER FACTORS OF THE ENVIRONMENT ON GROWTH AND REPRODUCTION IN PLANTS1Monthly Weather Review, 48
(2005)
BIOINFORMATICS APPLICATIONS NOTE Sequence analysis r2cat: synteny plots and comparative assembly
M. Kanehisa, S. Goto, S. Kawashima, Y. Okuno, M. Hattori (2004)
The KEGG resource for deciphering the genomeNucleic acids research, 32 Database issue
N. González-Schain, M. Diaz-Mendoza, Marek Żurczak, P. Suárez-López (2012)
Potato CONSTANS is involved in photoperiodic tuberization in a graft-transmissible manner.The Plant journal : for cell and molecular biology, 70 4
F. Turck, F. Fornara, G. Coupland (2008)
Regulation and identity of florigen: FLOWERING LOCUS T moves center stage.Annual review of plant biology, 59
Y. Yim, P. Moak, Hector Sanchez-Villeda, T. Musket, P. Close, P. Klein, J. Mullet, M. McMullen, Zheiwei Fang, M. Schaeffer, J. Gardiner, E. Coe, G. Davis (2007)
A BAC pooling strategy combined with PCR-based screenings in a large, highly repetitive genome enables integration of the maize genetic and physical mapsBMC Genomics, 8
Maria Albani, G. Coupland (2010)
Comparative analysis of flowering in annual and perennial plants.Current topics in developmental biology, 91
Xiao-Fei Cheng, Zeng‐Yu Wang (2005)
Overexpression of COL9, a CONSTANS-LIKE gene, delays flowering by reducing expression of CO and FT in Arabidopsis thaliana.The Plant journal : for cell and molecular biology, 43 5
Alberto Cenci, S. Somma, Nathalie Chantret, Jorge Dubcovsky, Alicia Blanco (2004)
PCR identification of durum wheat BAC clones containing genes coding for carotenoid biosynthesis enzymes and their chromosome localization.Genome, 47 5
Ravi Patel, Mukesh Jain (2012)
NGS QC Toolkit: A Toolkit for Quality Control of Next Generation Sequencing DataPLoS ONE, 7
C. Su, Y. Chao, Yao-Chien Chang, Wan-Chieh Chen, Chun-Yi Chen, Ann-Ying Lee, Kee Hwa, M. Shih (2011)
De novo assembly of expressed transcripts and global analysis of the Phalaenopsis aphrodite transcriptome.Plant & cell physiology, 52 9
A. Holefors, Lars Opseth, A. Rosnes, Linda Ripel, L. Snipen, Carl Fossdal, J. Olsen (2009)
Identification of PaCOL1 and PaCOL2, two CONSTANS-like genes showing decreased transcript levels preceding short day induced growth cessation in Norway spruce.Plant physiology and biochemistry : PPB, 47 2
Evangeline Ballerini, E. Kramer (2011)
In the Light of Evolution: A Reevaluation of Conservation in the CO–FT Regulon and Its Role in Photoperiodic Regulation of Flowering TimeFrontiers in plant science, 2
T. Chia, A. Müller, C. Jung, Euphemia Mutasa-Gottgens (2008)
Sugar beet contains a large CONSTANS-LIKE gene family including a CO homologue that is independent of the early-bolting (B) gene locusJournal of Experimental Botany, 59
YY Chang, NH Kao, JY Li, WH Hsu, YL Liang, JW Wu, CH Yang (2010)
Characterization of the possible roles for B Class MADS box genes in regulation of perianth formation in Orchid (Oncidium Gower Ramsey)Plant Physiol, 152
M. Yano, Y. Katayose, M. Ashikari, U. Yamanouchi, L. Monna, T. Fuse, T. Baba, Kimiko Yamamoto, Y. Umehara, Y. Nagamura, Takuji Sasaki (2000)
Hd1, a Major Photoperiod Sensitivity Quantitative Trait Locus in Rice, Is Closely Related to the Arabidopsis Flowering Time Gene CONSTANSPlant Cell, 12
M. Febrer, E. Wilhelm, N. Al-Kaff, Jonathan Wright, W. Powell, M. Bevan, M. Boulton (2009)
Rapid identification of the three homoeologues of the wheat dwarfing gene Rht using a novel PCR-based screen of three-dimensional BAC pools.Genome, 52 12
Smita Kurup, Huw Jones, Michael Holdsworth (2000)
Interactions of the developmental regulator ABI3 with proteins identified from developing Arabidopsis seeds.The Plant journal : for cell and molecular biology, 21 2
O. Morozova, M. Marra (2008)
Applications of next-generation sequencing technologies in functional genomics.Genomics, 92 5
Zhangying Wang, B. Fang, Jingyi Chen, Xiongjian Zhang, Zhongxia Luo, Lifei Huang, Xinliang Chen, Yujun Li (2010)
De novo assembly and characterization of root transcriptome using Illumina paired-end sequencing and development of cSSR markers in sweetpotato (Ipomoea batatas)BMC Genomics, 11
T. Bailey, M. Bodén, Fabian Buske, M. Frith, Charles Grant, Luca Clementi, Jingyuan Ren, Wilfred Li, William Noble (2009)
MEME Suite: tools for motif discovery and searchingNucleic Acids Research, 37
Yu-Yun Chang, Yi-Feng Chiu, Jia-Wei Wu, Chang-Hsien Yang (2009)
Four orchid (Oncidium Gower Ramsey) AP1/AGL9-like MADS box genes show novel expression patterns and cause different effects on floral transition and formation in Arabidopsis thaliana.Plant & cell physiology, 50 8
Manuel Garber, M. Grabherr, M. Guttman, C. Trapnell (2011)
Computational methods for transcriptome annotation and quantification using RNA-seqNature Methods, 8
G. Pellegrino, F. Bellusci, A. Musacchio (2009)
Genetic integrity of sympatric hybridising plant species: the case of Orchis italica and O. anthropophora.Plant biology, 11 3
K. Tamura, J. Dudley, M. Nei, Sudhir Kumar (2007)
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.Molecular biology and evolution, 24 8
B. Thomas (2006)
Light signals and flowering.Journal of experimental botany, 57 13
T. Imaizumi, S. Kay (2006)
Photoperiodic control of flowering: not only by coincidence.Trends in plant science, 11 11
Yu-Yun Chang, Yen-Wei Chu, Chi-Wei Chen, W. Leu, Hsing-Fun Hsu, Chang-Hsien Yang (2011)
Characterization of Oncidium 'Gower Ramsey' transcriptomes using 454 GS-FLX pyrosequencing and their application to the identification of genes associated with flowering time.Plant & cell physiology, 52 9
C. Strayer, T. Oyama, T. Schultz, Ramanujam Raman, D. Somers, P. Más, Satchidananda Panda, J. Kreps, S. Kay (2000)
Cloning of the Arabidopsis clock gene TOC1, an autoregulatory response regulator homolog.Science, 289 5480
M. Margulies, M. Egholm, William Altman, S. Attiya, J. Bader, Lisa Bemben, J. Berka, Michael Braverman, Yi-Ju Chen, Zhoutao Chen, Scott Dewell, Lei Du, J. Fierro, Xavier Gomes, B. Godwin, Wenshe He, S. Helgesen, Chun Ho, G. Irzyk, Szilveszter Jando, Maria Alenquer, T. Jarvie, K. Jirage, Jong-Bum Kim, James Knight, Janna Lanza, J. Leamon, S. Lefkowitz, M. Lei, Jing Li, K. Lohman, Hong Lu, V. Makhijani, K. McDade, M. McKenna, E. Myers, E. Nickerson, J. Nobile, Ramona Plant, Bernard Puc, M. Ronan, G. Roth, G. Sarkis, J. Simons, J. Simpson, Maithreyan Srinivasan, K. Tartaro, A. Tomasz, K. Vogt, G. Volkmer, Shally Wang, Yong Wang, M. Weiner, Pengguang Yu, R. Begley, J. Rothberg (2005)
Genome sequencing in microfabricated high-density picolitre reactorsNature, 437
Ryosuke Hayama, G. Coupland (2004)
The Molecular Basis of Diversity in the Photoperiodic Flowering Responses of Arabidopsis and RicePlant Physiology, 135
Xiudong Sun, Shumei Zhou, Fanlu Meng, Shiqi Liu (2012)
De novo assembly and characterization of the garlic (Allium sativum) bud transcriptome by Illumina sequencingPlant Cell Reports, 31
Yu-Yun Hsiao, Zhao-Jun Pan, Chia-Chi Hsu, Ya-Ping Yang, Yi-Chin Hsu, Yu‐Chen Chuang, H. Shih, Wen-huei Chen, W. Tsai, Hong-Hwa Chen (2011)
Research on orchid biology and biotechnology.Plant & cell physiology, 52 9
Dejun Li, Z. Deng, B. Qin, Xianghong Liu, Z. Men (2012)
De novo assembly and characterization of bark transcriptome using Illumina sequencing and development of EST-SSR markers in rubber tree (Hevea brasiliensis Muell. Arg.)BMC Genomics, 13
T. Yamashino, A. Matsushika, Toru Fujimori, Shusei Sato, Tomohiko Kato, S. Tabata, T. Mizuno (2003)
A Link between circadian-controlled bHLH factors and the APRR1/TOC1 quintet in Arabidopsis thaliana.Plant & cell physiology, 44 6
J. Thompson, D. Higgins, T. Gibson (1994)
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.Nucleic acids research, 22 22
R. Stevens (2014)
Gene Ontology Consortium
Chung-yi Chiou, K. Yeh (2008)
Differential expression of MYB gene (OgMYB1) determines color patterning in floral tissue of Oncidium Gower RamseyPlant Molecular Biology, 66
J. Putterill, F. Robson, Karen Lee, R. Simon, G. Coupland (1995)
The CONSTANS gene of arabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factorsCell, 80
Yu-Yun Chang, Nai-Hsuan Kao, Jen-Ying Li, Wei-Han Hsu, Yu-Ling Liang, Jia-Wei Wu, Chang-Hsien Yang (2009)
Characterization of the Possible Roles for B Class MADS Box Genes in Regulation of Perianth Formation in Orchid1[C]Plant Physiology, 152
M. Delseny, B. Han, Y. Hsing (2010)
High throughput DNA sequencing: The new sequencing revolution.Plant science : an international journal of experimental plant biology, 179 5
F. Pinheiro, F. Barros, C. Palma‐Silva, D. Meyer, M. Fay, R. Suzuki, C. Lexer, S. Cozzolino (2010)
Hybridization and introgression across different ploidy levels in the Neotropical orchids Epidendrum fulgens and E. puniceoluteum (Orchidaceae)Molecular Ecology, 19
M. Thiruvengadam, Chang-Hsien Yang (2009)
Ectopic expression of two MADS box genes from orchid (Oncidium Gower Ramsey) and lily (Lilium longiflorum) alters flower transition and formation in Eustoma grandiflorumPlant Cell Reports, 28
Chiara Campoli, B. Drosse, I. Searle, G. Coupland, M. Korff (2012)
Functional characterisation of HvCO1, the barley (Hordeum vulgare) flowering time ortholog of CONSTANS.The Plant journal : for cell and molecular biology, 69 5
M. Kanehisa, M. Araki, S. Goto, M. Hattori, M. Hirakawa, M. Itoh, Toshiaki Katayama, S. Kawashima, Shujiro Okuda, T. Tokimatsu, Yoshihiro Yamanishi (2007)
KEGG for linking genomes to life and the environmentNucleic Acids Research, 36
Hsing-Fun Hsu, Chih‐Hsiang Huang, Lucy Chou, Chang-Hsien Yang (2003)
Ectopic expression of an orchid (Oncidium Gower Ramsey) AGL6-like gene promotes flowering by activating flowering time genes in Arabidopsis thaliana.Plant & cell physiology, 44 8
G. Simpson, C. Dean (2002)
Arabidopsis, the Rosetta stone of flowering time?Science, 296 5566
Jianxia Zhang, Kunjun Wu, L. Tian, S. Zeng, J. Duan (2011)
Cloning and characterization of a novel CONSTANS-like gene from Phalaenopsis hybridaActa Physiologiae Plantarum, 33
S. Hepworth, F. Valverde, Dean Ravenscroft, A. Mouradov, G. Coupland (2002)
Antagonistic regulation of flowering‐time gene SOC1 by CONSTANS and FLC via separate promoter motifsThe EMBO Journal, 21
Susan Ledger, C. Strayer, Fern Ashton, Steve Kay, Jo Putterill (2001)
Analysis of the function of two circadian-regulated CONSTANS-LIKE genes.The Plant journal : for cell and molecular biology, 26 1
P. Wall, Jim Leebens-Mack, Andre Chanderbali, Abdelali Barakat, Erik Wolcott, Haiying Liang, Lena Landherr, L. Tomsho, Y. Hu, John Carlson, Hong Ma, Stephan Schuster, D. Soltis, P. Soltis, Naomi Altman, C. dePamphilis (2009)
Comparison of next generation sequencing technologies for transcriptome characterizationBMC Genomics, 10
S. Yang, Z. Tu, F. Cheung, W. Xu, J. Lamb, J. Lamb, Hansang Jung, Hansang Jung, C. Vance, C. Vance, J. Gronwald, J. Gronwald (2011)
Using RNA-Seq for gene identification, polymorphism detection and transcript profiling in two alfalfa genotypes with divergent cell wall composition in stemsBMC Genomics, 12
(2013)
Planta
Y. Nemoto, M. Kisaka, T. Fuse, M. Yano, Y. Ogihara (2003)
Characterization and functional analysis of three wheat genes with homology to the CONSTANS flowering time gene in transgenic rice.The Plant journal : for cell and molecular biology, 36 1
K. Link, Florian Vogel, U. Sonnewald (2011)
PD Trafficking of Potato Leaf Roll Virus Movement Protein in Arabidopsis Depends on Site-specific Protein PhosphorylationFrontiers in plant science, 2
The high chromosome numbers, polyploid genomes, and long juvenile phases of most ornamental orchid species render functional genomics difficult and limit the discovery of genes influencing horticultural traits. The orchid Erycina pusilla has a low chromosome number (2n = 12) and flowers in vitro within 1 year, making it a standout candidate for use as a model orchid. However, transcriptomic and genomic information from E. pusilla remains limited. In this study, next-generation sequencing (NGS) technology was used to identify 90,668 unigenes by de novo assembly. These unigenes were annotated functionally and analyzed with regard to their gene ontology (GO), clusters of orthologous groups (COG), and KEGG pathways. To validate the discovery methods, a homolog of CONSTANS (CO), one of the key genes in the flowering pathway, was further analyzed. The Arabidopsis CO-Like (COL) amino acid sequences were used to screen for homologs in the E. pusilla transcriptome database. Specific primers to the homologous unigenes were then used to isolate BAC clones, which were sequenced to identify 12 E. pusilla CO-like (EpCOL) full-length genes. Based on sequence homology, domain structure, and phylogenetic analysis, these EpCOL genes were divided into four groups. Four EpCOLs fused with GFP were localized in the nucleus. Some EpCOL genes were regulated by light. These results demonstrate that nascent E. pusilla resources (transcriptome and BAC library) can be used to investigate the E. pusilla photoperiod-dependent flowering genes. In future, this strategy can be applied to other biological processes, marketable traits, and molecular breeding in this model orchid.
Planta – Springer Journals
Published: Feb 16, 2013
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.