Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/springer-journals/transgenic-indica-rice-plants-harboring-a-synthetic-cry2a-gene-of-fs0iRF7bl3
References (37)
-
Yongjun Lin, Qifa Zhang (2004)
Optimising the tissue culture conditions for high efficiency transformation of indica ricePlant Cell Reports, 23
-
MB Cohen (2000)
4Int Rice Res Notes, 25
-
F. Gould (2003)
Bt-resistance management—theory meets dataNature Biotechnology, 21
-
X. Duan, Xiaogang Li, Q. Xue, Mahmoud Abo-EI-Saad, Deping Xu, R. Wu (1996)
Transgenic rice plants harboring an introduced potato proteinase inhibitor II gene are insect resistantNature Biotechnology, 14
-
X. Cheng (1998)
Agrobacterium-transformed rice plants expressing synthetic cry1Ab and cry1Ac genes are highly toxic to yellow stem borer and striped stem borer, 95
-
J. Ferré, J. Rie (2002)
Biochemistry and Genetics of Insect Resistance to Bacillus thuringiensisAnnual Review of Entomology, 47
-
Xiongying Cheng, R. Sardana, Harvey Kaplan, I. Altosaar (1998)
Agrobacterium-transformed rice plants expressing synthetic cryIA(b) and cryIA(c) genes are highly toxic to striped stem borer and yellow stem borer.Proceedings of the National Academy of Sciences of the United States of America, 95 6
-
S. Maqbool, S. Riazuddin, N. Lộc, A. Gatehouse, J. Gatehouse, P. Christou (2004)
Expression of multiple insecticidal genes confers broad resistance against a range of different rice pestsMolecular Breeding, 7
-
B. Tabashnik, Y. Carrière, T. Dennehy, S. Morin, M. Sisterson, R. Roush, A. Shelton, Jian-Zhou Zhao (2003)
Insect Resistance to Transgenic Bt Crops: Lessons from the Laboratory and Field, 96
-
Michael Cohen, F. Gould, J. Bentur (2000)
Bt rice: practical steps to sustainable use.International Rice Research Notes, 25
-
S. Karim, D. Dean (2000)
Toxicity and Receptor Binding Properties of Bacillus thuringiensisδ-Endotoxins to the Midgut Brush Border Membrane Vesicles of the Rice Leaf Folders, Cnaphalocrocis medinalis and Marasmia patnalisCurrent Microbiology, 41
-
J. Breitler, V. Marfâ, M. Royer, D. Meynard, J. Vassal, B. Vercambre, R. Frutos, J. Messeguer, R. Gabarra, E. Guiderdoni (2000)
Expression of a Bacillus thuringiensis cry1B synthetic gene protects Mediterranean rice against the striped stem borerPlant Cell Reports, 19
-
B. Tabashnik, T. Dennehy, M. Sims, K. Larkin, G. Head, W. Moar, Y. Carrière (2002)
Control of Resistant Pink Bollworm (Pectinophora gossypiella) by Transgenic Cotton That Produces Bacillus thuringiensis Toxin Cry2AbApplied and Environmental Microbiology, 68
-
R. Roush (1998)
Two-toxin strategies for management of insecticidal transgenic crops: can pyramiding succeed where pesticide mixtures have not?Philosophical Transactions of the Royal Society B, 353
-
P. Nayak, D. Basu, Sampa Das, Asitava Basu, Dipankar Ghosh, Neeliyath Ramakrishnan, Maloy Ghosh, S. Sen (1997)
Transgenic elite indica rice plants expressing CryIAc delta-endotoxin of Bacillus thuringiensis are resistant against yellow stem borer (Scirpophaga incertulas).Proceedings of the National Academy of Sciences of the United States of America, 94 6
-
E. Alcantara, R. Aguda, A. Curtiss, D. Dean, Michael Cohen (2004)
Bacillus thuringiensis delta-endotoxin binding to brush border membrane vesicles of rice stem borers.Archives of insect biochemistry and physiology, 55 4
-
R. Frutos, C. Rang, M. Royer (1999)
Managing Insect Resistance to Plants Producing Bacillus thuringiensis ToxinsCritical Reviews in Biotechnology, 19
-
S. Bates, Jian-Zhou Zhao, R. Roush, A. Shelton (2005)
Insect resistance management in GM crops: past, present and futureNature Biotechnology, 23
-
M. Murray, W. Thompson (1980)
Rapid isolation of high molecular weight plant DNA.Nucleic acids research, 8 19
-
A. Shelton, Jian-Zhou Zhao, R. Roush (2002)
Economic, ecological, food safety, and social consequences of the deployment of bt transgenic plants.Annual review of entomology, 47
-
X. Qi, F. Fufa, D. Sijtsma, R. Niks, P. Lindhout, P. Stam (2000)
The evidence for abundance of QTLs for partial resistance to Puccinia hordei on the barley genomeMolecular Breeding, 6
-
Kede Liu, Jing Wang, Haitao Li, C. Xu, A. Liu, X. Li, Qinglu Zhang (1997)
A genome-wide analysis of wide compatibility in rice and the precise location of the S5 locus in the molecular mapTheoretical and Applied Genetics, 95
-
T Murashige, F Skoog (1962)
A revised medium for rapid growth and bioassays with tobacco culturesPlant Physiol, 15
-
T. Murashige, F. Skoog (1962)
A revised medium for rapid growth and bio assays with tobacco tissue culturesPhysiologia Plantarum, 15
-
Jian-Zhou Zhao, Jun Cao, Yaxin Li, Hilda Collins, R. Roush, E. Earle, A. Shelton (2003)
Transgenic plants expressing two Bacillus thuringiensis toxins delay insect resistance evolutionNature Biotechnology, 21
-
S. Maqbool, T. Husnain, S. Riazuddin, L. Masson, Paul Christou (1998)
Effective control of yellow stem borer and rice leaf folder in transgenic rice indica varieties Basmati 370 and M 7 using the novel δ-endotoxin cry2A Bacillus thuringiensis geneMolecular Breeding, 4
-
MG Murray, WF Thompson (1980)
Isolation of high molecular weight plant DNANucl Acid Res, 19
-
Khurram Bashir, T. Husnain, Tahira Fatima, Z. Latif, Syed Mehdi, S. Riazuddin (2004)
Field evaluation and risk assessment of transgenic indica basmati riceMolecular Breeding, 13
-
RT Roush (1998)
Two-toxin strategies for management of insecticidal transgenic crops: can pyramiding succeed where pesticide mixtures have notPhil Trans R Soc Lond B, 35
-
Maike Tam, J. Oseph, N. Mol, M. Ooter, Biocentrum Amsterdam (1997)
THE SILENCE OF GENES IN TRANSGENIC PLANTSAnnals of Botany, 79
-
J. Tu, Guoan Zhang, K. Datta, Cai-guo Xu, Yuqing He, Qifa Zhang, G. Khush, S. Datta (2000)
Field performance of transgenic elite commercial hybrid rice expressing Bacillus thuringiensis δ-endotoxinNature Biotechnology, 18
-
T. Husnain, Jan Asad, S. Maqbool, S. Datta, S. Riazuddin (2002)
Variability in expression of insecticidal Cry1Ab gene in Indica Basmati riceEuphytica, 128
-
G. Yè, Q. Shu, Hong Yao, H. Cui, Xi Cheng, Cui Hu, Ying-wu Xia, M. Gao, I. Altosaar (2001)
Field Evaluation of Resistance of Transgenic Rice Containing a Synthetic cry1Ab Gene from Bacillus thuringiensis Berliner to Two Stem Borers, 94
-
S. Ramesh, D. Nagadhara, I. Pasalu, A. Kumari, N. Sarma, V. Reddy, K. Rao (2004)
Development of stem borer resistant transgenic parental lines involved in the production of hybrid rice.Journal of biotechnology, 111 2
-
Zhonghua Wang, Q. Shu, G. Yè, H. Cui, Dianxing Wu, I. Altosaar, Ying-wu Xia (2002)
Genetic analysis of resistance of Bt rice to stripe stem borer (Chilo suppressalis)Euphytica, 123
-
Sasha High, Michael Cohen, Q. Shu, I. Altosaar (2004)
Achieving successful deployment of Bt rice.Trends in plant science, 9 6
-
M. Stam, J. Mol, J. Kooter (1997)
Review Article: The Silence of Genes in Transgenic PlantsAnnals of Botany, 79
- Publisher
- Springer Journals
- Copyright
- Copyright © 2005 by Springer-Verlag
- Subject
- Life Sciences; Biotechnology; Biochemistry, general; Plant Biochemistry; Agriculture; Plant Genetics & Genomics; Plant Breeding/Biotechnology
- ISSN
- 0040-5752
- eISSN
- 1432-2242
- DOI
- 10.1007/s00122-005-0062-8
- pmid
- 16187120
- Publisher site
- See Article on Publisher Site
Abstract
A novel synthetic cry2A* gene was introduced into the elite indica rice restorer line Minghui 63 by Agrobacterium -mediated transformation. A total of 102 independent transformants were obtained. Among them, 71 transformants were positive cry2A* plants according to PCR analysis. Four highly insect-resistant lines with single-copy insertion (designated as 2A-1, 2A-2, 2A-3, and 2A-4) were selected based on field assessment and Southern blot analysis in the T 1 generation. All four transgenic lines showed Mendelian segregation by seed germination on 1/2 MS medium containing Basta. Homozygous transgenic plants were selected according to germination ratio (100%) in the T 2 generation. Cry2A* protein concentrations were determined in homozygous transgenic lines, their derived hybrids, and their backcross offspring. The Cry2A* protein concentrations of four homozygous transgenic lines ranged from 9.65 to 12.11 μg/g of leaf fresh weight. There was little variation in the hybrids and backcross offspring. Insect bioassays were conducted in both the laboratory and field. All four transgenic lines were significantly resistant to lepidopteran rice pests. These cry2A* transgenic lines can be used to produce insect-resistant hybrids and serve as a resistant source for the development of two-toxin Bt rice.
Journal
TAG Theoretical and Applied Genetics – Springer Journals
Published: Nov 1, 2005