Access the full text.
Sign up today, get DeepDyve free for 14 days.
H. Kwon, L. Saif, D. Jackwood (1998)
Field isolates of transmissible gastroenteritis virus differ at the molecular level from the Miller and Purdue virulent and attenuated strains and from porcine respiratory coronaviruses.The Journal of veterinary medical science, 60 5
H. Laude, K. Reeth, M. Pensaert (1993)
Porcine respiratory coronavirus: molecular features and virus-host interactions.Veterinary research, 24 2
M. Ballesteros, C. Sánchez, L. Enjuanes (1997)
Two Amino Acid Changes at the N-Terminus of Transmissible Gastroenteritis Coronavirus Spike Protein Result in the Loss of Enteric TropismVirology, 227
R. Wesley, R. Woods, A. Cheung (1991)
Genetic analysis of porcine respiratory coronavirus, an attenuated variant of transmissible gastroenteritis virusJournal of Virology, 65
J. O'Connor, D. Brian (1999)
The Major Product of Porcine Transmissible Gastroenteritis Coronavirus Gene 3b Is an Integral Membrane Glycoprotein of 31 kDa☆Virology, 256
K. Page, K. Mawditt, P. Britton (1991)
Sequence comparison of the 5' end of mRNA 3 from transmissible gastroenteritis virus and porcine respiratory coronavirus.The Journal of general virology, 72 ( Pt 3)
R. Wesley, R. Woods, A. Cheung (1990)
Genetic basis for the pathogenesis of transmissible gastroenteritis virusJournal of Virology, 64
P. Paul, E. Vaughn, P. Halbur (1997)
Pathogenicity and sequence analysis studies suggest potential role of gene 3 in virulence of swine enteric and respiratory coronaviruses.Advances in experimental medicine and biology, 412
P. Chomczyński, N. Sacchi (1987)
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.Analytical biochemistry, 162 1
Serge Bernard, Hubert Laude (1995)
Site-specific alteration of transmissible gastroenteritis virus spike protein results in markedly reduced pathogenicity.The Journal of general virology, 76 ( Pt 9)
E. Vaughn, P. Halbur, P. Paul (1995)
Sequence comparison of porcine respiratory coronavirus isolates reveals heterogeneity in the S, 3, and 3-1 genesJournal of Virology, 69
R. Wesley (1990)
Nucleotide sequence of the E2-peplomer protein gene and partial nucleotide sequence of the upstream polymerase gene of transmissible gas gastroenteritis virus (Miller strain).Advances in experimental medicine and biology, 276
L. Saif, J. Cott, T. Brim (1994)
Immunity to transmissible gastroenteritis virus and porcine respiratory coronavirus infections in swineVeterinary Immunology and Immunopathology, 43
R. Wesley, A. Cheung, David Michael, R. Woods (1989)
Nucleotide sequence of coronavirus TGEV genomic RNA: evidence for 3 mRNA species between the peplomer and matrix protein genesVirus Research, 13
B. Delmas, D. Rasschaert, M. Godet, J. Gelfi, H. Laude (1990)
Four major antigenic sites of the coronavirus transmissible gastroenteritis virus are located on the amino-terminal half of spike glycoprotein S.The Journal of general virology, 71 ( Pt 6)
B. Schultze, C. Krempl, M. Ballesteros, L. Shaw, R. Schauer, L. Enjuanes, G. Herrler (1996)
Transmissible gastroenteritis coronavirus, but not the related porcine respiratory coronavirus, has a sialic acid (N-glycolylneuraminic acid) binding activityJournal of Virology, 70
G. Pritchard, D. Paton, G. Wibberley, G. Ibata (1999)
Transmissible gastroenteritis and porcine epidemic diarrhoea in BritainVeterinary Record, 144
A. McGoldrick, J. Lowings, D. Paton (2014)
Characterisation of a recent virulent transmissible gastroenteritis virus from Britain with a deleted ORF 3aArchives of Virology, 144
P. Kapke, D. Brian (1986)
Sequence analysis of the porcine transmissible gastroenteritis coronavirus nucleocapsid protein geneVirology, 151
W. Spaan, D. Cavanagh, Marian Horzinek (1988)
Coronaviruses: structure and genome expression.The Journal of general virology, 69 ( Pt 12)
P. Britton, K. Page (1990)
Sequence of the S gene from a virulent British field isolate of transmissible gastroenteritis virusVirus Research, 18
D. Paton, G. Ibata, J. Sands, A. McGoldrick (1997)
Detection of transmissible gastroenteritis virus by RT-PCR and differentiation from porcine respiratory coronavirusJournal of Virological Methods, 66
J. Vancott, T. Brim, J. Lunney, L. Saif (1994)
Contribution of antibody-secreting cells induced in mucosal lymphoid tissues of pigs inoculated with respiratory or enteric strains of coronavirus to immunity against enteric coronavirus challenge.Journal of immunology, 152 8
C. Sánchez, F. Gebauer, C. Suñé, A. Méndez, J. Dopazo, L. Enjuanes (1992)
Genetic evolution and tropism of transmissible gastroenteritis coronavirusesVirology, 190
C. Krempl, B. Schultze, H. Laude, G. Herrler (1997)
Point mutations in the S protein connect the sialic acid binding activity with the enteropathogenicity of transmissible gastroenteritis coronavirusJournal of Virology, 71
M. Godet, R. L'Haridon, J. Vautherot, H. Laude (1992)
TGEV corona virus ORF4 encodes a membrane protein that is incorporated into virionsVirology, 188
B. Eh, L. Saif, K. Theil, Agnes Ag, Cross Rf (1982)
Porcine pararotavirus: detection, differentiation from rotavirus, and pathogenesis in gnotobiotic pigsJournal of Clinical Microbiology, 15
R. Simkins, P. Weilnau, J. Bias, L. Saif (1992)
Antigenic variation among transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus strains detected with monoclonal antibodies to the S protein of TGEV.American journal of veterinary research, 53 7
L. Jacobs, B. Zeijst, Marian Horzinek (1986)
Characterization and translation of transmissible gastroenteritis virus mRNAsJournal of Virology, 57
(1990)
Intestinal replication of PRCV closely related antigenically to the enteric transmissible gastroenteritis virus
TGEV replicates in intestinal enterocytes and causes diarrhea in young pigs. PRCV, a spike (S) gene deletion mutant of TGEV with an altered respiratory tissue tropism, causes mild or subclinical respiratory infections. Comparisons of TGEV and PRCV strains suggest that tropism and pathogenicity are influenced by the S gene and ORF3, respectively. Recently, outbreaks of TGE of reduced virulence were reported in the field. We investigated a similar suspect TGEV outbreak of reduced virulence in nursery pigs from a swine herd in the Midwest. A TGEV strain (BW021898B) was isolated in swine testicular cells from gut contents of a diarrheic pig and three PRCV strains (BW126, BW154, BW155) were isolated from nasal swabs from normal TGEV-seronegative sentinel pigs in contact with the diarrheic pigs. Sequence analysis of the TGEV isolate in the partial S gene and ORF3/3a and ORF3-1/3b revealed high homology with enteropathogenic TGEV strains. Gnotobiotic pig inoculation and histopathological results revealed that this TGEV isolate retained virulence even though in the field outbreak the diarrheal disease was of reduced severity. Sequence analysis of the S gene deletion region of the three PRCV isolates revealed identical deletions between nt 105–752, which differ from deletions previously reported among PRCV strains. The three PRCV isolates had variable sequence changes in ORF 3/3a and ORF 3-1/3b, affecting the ORF size and amino acid sequence. Thus, sequence analysis and pathogenicity studies indicate that this TGEV isolate resembles other enteropathogenic TGEV strains. Therefore, the reduced severity of TGE observed in this herd may be due to the ongoing PRCV infections, which induce antibodies cross-reactive with TGEV and result in decreased disease severity. The results outlined in this study highlight the need to monitor the molecular epidemiology of TGEV/PRCV strains with sensitive differential diagnostic assays, followed by sequence analysis of the critical regions to identify changes and pathogenicity studies to confirm the disease potential of the TGEV isolates.
Archives of Virology – Springer Journals
Published: Jun 1, 2000
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.