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Chlorophyll Fluorescence: A Probe of Photosynthesis In Vivo

Chlorophyll Fluorescence: A Probe of Photosynthesis In Vivo The use of chlorophyll fluorescence to monitor photosynthetic performance in algae and plants is now widespread. This review examines how fluorescence parameters can be used to evaluate changes in photosystem II (PSII) photochemistry, linear electron flux, and CO 2 assimilation in vivo, and outlines the theoretical bases for the use of specific fluorescence parameters. Although fluorescence parameters can be measured easily, many potential problems may arise when they are applied to predict changes in photosynthetic performance. In particular, consideration is given to problems associated with accurate estimation of the PSII operating efficiency measured by fluorescence and its relationship with the rates of linear electron flux and CO 2 assimilation. The roles of photochemical and nonphotochemical quenching in the determination of changes in PSII operating efficiency are examined. Finally, applications of fluorescence imaging to studies of photosynthetic heterogeneity and the rapid screening of large numbers of plants for perturbations in photosynthesis and associated metabolism are considered. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Annual Review of Plant Biology Annual Reviews

Chlorophyll Fluorescence: A Probe of Photosynthesis In Vivo

Annual Review of Plant Biology , Volume 59 – Jun 2, 2008

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References (109)

Publisher
Annual Reviews
Copyright
Copyright © 2008 by Annual Reviews. All rights reserved
ISSN
1040-2519
DOI
10.1146/annurev.arplant.59.032607.092759
pmid
18444897
Publisher site
See Article on Publisher Site

Abstract

The use of chlorophyll fluorescence to monitor photosynthetic performance in algae and plants is now widespread. This review examines how fluorescence parameters can be used to evaluate changes in photosystem II (PSII) photochemistry, linear electron flux, and CO 2 assimilation in vivo, and outlines the theoretical bases for the use of specific fluorescence parameters. Although fluorescence parameters can be measured easily, many potential problems may arise when they are applied to predict changes in photosynthetic performance. In particular, consideration is given to problems associated with accurate estimation of the PSII operating efficiency measured by fluorescence and its relationship with the rates of linear electron flux and CO 2 assimilation. The roles of photochemical and nonphotochemical quenching in the determination of changes in PSII operating efficiency are examined. Finally, applications of fluorescence imaging to studies of photosynthetic heterogeneity and the rapid screening of large numbers of plants for perturbations in photosynthesis and associated metabolism are considered.

Journal

Annual Review of Plant BiologyAnnual Reviews

Published: Jun 2, 2008

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