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References (34)
-
D. Fischhoff, K. Bowdish, F. Perlak, P. Marrone, S. McCormick, J. Niedermeyer, Duff Dean, K. Kusano-Kretzmer, E. Mayer, D. Rochester, S. Rogers, R. Fraley (1987)
Insect Tolerant Transgenic Tomato PlantsBio/Technology, 5
-
A. Klier, F. Fargette, J. Ribier, G. Rapoport (1982)
Cloning and expression of the crystal protein genes from Bacillus thuringiensis strain berliner 1715.The EMBO Journal, 1
-
Fran�oise Jaquet, Ralf H�tter, Peter L�thy (1987)
Specificity of Bacillus thuringiensis Delta-EndotoxinApplied and Environmental Microbiology, 53
-
H. Schnepf, H. Whiteley (1981)
Cloning and expression of the Bacillus thuringiensis crystal protein gene in Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America, 78 5
-
M. Adang, M. Staver, T. Rocheleau, J. Leighton, R. Barker, D. Thompson (1985)
Characterized full-length and truncated plasmid clones of the crystal protein of Bacillus thuringiensis subsp. kurstaki HD-73 and their toxicity to Manduca sexta.Gene, 36 3
-
G Certain (1989)
Star searchCotton Grower, 25
-
C. Bergsten (1974)
The Threat Is RealForeign Policy
-
K. Barton, W. Brill (1983)
Prospects in plant genetic engineering.Science, 219 4585
-
M. Vaeck, A. Reynaerts, H. Höfte, S. Jansens, M. Beuckeleer, C. Dean, M. Zabeau, M. Montagu, J. Leemans (1987)
Transgenic plants protected from insect attackNature, 328
-
Robert Kay, Amy Chan, M. Daly, J. Mcpherson (1987)
Duplication of CaMV 35S Promoter Sequences Creates a Strong Enhancer for Plant GenesScience, 236
-
NL Trolinder, JR Goodin (1987)
Somatic embryogenesis and plant regeneration in cotton (Gossypium hisutum L.)Plant Cell Reports, 6
-
M. Bevan, R. Flavell, M. Chilton (1983)
A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformationNature, 304
-
A. Aronson, W. Beckman, P. Dunn (1986)
Bacillus thuringiensis and related insect pathogens.Microbiological reviews, 50 1
-
E Firoozabady, DL DeBoer, DJ Merlo, EL Halk, LN Amerson, KE Rashka, EE Murray (1987)
Transformation of cotton (Gossypium hirsutum L.) by Agrobacterium tumefaciens and regeneration of transgenic plantsPlant Mol. Biol., 10
-
P. Umbeck, G. Johnson, K. Barton, W. Swain (1987)
Genetically Transformed Cotton (Gossypium Hirsutum L.) PlantsBio/Technology, 5
-
S. Macintosh, T. Stone, S. Sims, P. Hunst, J. Greenplate, P. Marrone, F. Perlak, D. Fischhoff, R. Fuchs (1990)
Specificity and efficacy of purified Bacillus thuringiensis proteins against agronomically important insects.Journal of invertebrate pathology, 56 2
-
G. Held, L. Bulla, E. Ferrari, J. Hoch, A. Aronson, S. Minnich (1982)
Cloning and localization of the lepidopteran protoxin gene of Bacillus thuringiensis subsp. kurstaki.Proceedings of the National Academy of Sciences of the United States of America, 79 19
-
HT Towbin, T Staelhelin, J Gordon (1979)
Electrophoretic transfer of proteins from polyacrylAmide gels to nitrocellulose sheetsProc. Natl. Acad. Sci. USA, 76
-
H. Towbin, T. Staehelin, J. Gordon (1979)
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences of the United States of America, 76 9
-
H. Seney (1984)
Integrated pest management for cotton in the western region of the United States. -
X. Delannay, B. LaVallee, Robert Proksch, R. Fuchs, S. Sims, J. Greenplate, P. Marrone, R. Dodson, J. Augustine, J. Layton, D. Fischhoff (1989)
Field Performance of Transgenic Tomato Plants Expressing the Bacillus Thuringiensis Var. Kurstaki Insect Control ProteinNature Biotechnology, 7
-
L. Herrera-Estrella, A. Depicker, M. Montagu, J. Schell (1983)
Expression of chimaeric genes transferred into plant cells using a Ti-plasmid-derived vectorNature, 303
-
M. Geiser, S. Schweitzer, C. Grimm (1986)
The hypervariable region in the genes coding for entomopathogenic crystal proteins of Bacillus thuringiensis: nucleotide sequence of the kurhd1 gene of subsp. kurstaki HD1.Gene, 48 1
-
T. Kunkel (1985)
Rapid and efficient site-specific mutagenesis without phenotypic selection.Proceedings of the National Academy of Sciences of the United States of America, 82 2
-
X Delannay, BJ LaVallee, RK Proksch, RL Fuchs, SR Sims, JT Greenplate, PG Marrone, RB Dodson, JJ Augustine, JG Layton, DA Fischhoff (1989)
Field performance of transgenic tomato plants expressing the Bacillus thuringiensis var. kurstaki insect control proteinBio/Technology, 7
-
SC MacIntosh, TB Stone, SR Sims, PL Hunst, JT Greenplate, PG Marrone, FJ Perlak, DA Fischhoff, RL Fuchs (1990)
J. Invert. Pathol -
K. Barton, A. Binns, A. Matzke, M. Chilton (1983)
Regeneration of intact tobacco plants containing full length copies of genetically engineered T-DNA, and transmission of T-DNA to R1 progenyCell, 32
-
L Suguiyama, C Osteen (1988)
The economic importance of cotton insects and mites -
R. Fraley, S. Rogers, R. Horsch, P. Sanders, J. Flick, S. Adams, M. Bittner, L. Brand, C. Fink, J. Fry, G. Galluppi, S. Goldberg, N. Hoffmann, S. Woo (1983)
Expression of bacterial genes in plant cells.Proceedings of the National Academy of Sciences of the United States of America, 80 15
-
W Spencer (1989)
The threat is realCotton Grower, 25
-
H. Höfte, J. Rie, S. Jansens, A. Houtven, H. Vanderbruggen, M. Vaeck (1988)
Monoclonal Antibody Analysis and Insecticidal Spectrum of Three Types of Lepidopteran-Specific Insecticidal Crystal Proteins of Bacillus thuringiensisApplied and Environmental Microbiology, 54
-
M. Hinchee, Dannette Connor-Ward, C. Newell, R. Mcdonnell, Shirley Sato, C. Gasser, D. Fischhoff, D. Re, R. Fraley, R. Horsch (1988)
Production of Transgenic Soybean Plants Using Agrobacterium-Mediated DNA TransferBio/Technology, 6
-
H. Whiteley, J. Kronstad, H. Schnepf, J. Desrosier (1982)
Cloning the Crystal Protein Gene of B. thuringiensis in E. coli -
RL Fuchs, SC MacIntosh, DA Dean, JT Greenplate, FJ Perlak, JC Pershing, PG Marrone, DA Fischhoff (1990)
Quantitation of Bacillus thuringiensis insect control protein as expressed in transgenic plants
- Publisher
- Springer Journals
- Copyright
- Copyright © 1990 by Nature Publishing Company
- Subject
- Life Sciences; Life Sciences, general; Biotechnology; Biomedicine, general; Agriculture; Biomedical Engineering/Biotechnology; Bioinformatics
- ISSN
- 1087-0156
- eISSN
- 1546-1696
- DOI
- 10.1038/nbt1090-939
- Publisher site
- See Article on Publisher Site
Abstract
We have expressed truncated forms of the insect control protein genes of Bacillus thuringiensis var. kurstaki HD-1(cryIA(b)) and HD-73 (cryIA(c)) in cotton plants at levels that provided effective control of agronomically important lepidopteran insect pests. Total protection from insect damage of leaf tissue from these plants was observed in laboratory assays when tested with two lepidopteran insects, an insect relatively sensitive to the B.t.k. insect control protein, Trichoplusia ni (cabbage looper) and an insect that is 100 fold less sensitive, Spodoptera exigua (beet armyworm). Whole plants, assayed under conditions of high insect pressure with Heliothis zea (cotton bollworm) showed effective square and boll protection. Immunological analysis of the cotton plants indicated that the insect control protein represented 0.05% to 0.1% of the total soluble protein. We view these results as a major step towards the agricultural use of genetically modified plants with insect resistance in this valuable, high acreage crop.
Journal
Nature Biotechnology – Springer Journals
Published: Oct 1, 1990