Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

RFLP-based genetic relationships of Einkorn wheats

RFLP-based genetic relationships of Einkorn wheats 122 88 88 6 7 R. Castagna G. Maga M. Perenzin M. Heun F. Salamini Istituto Sperimentale per la Cerealicoltura Sezione di S.Angelo Lodigiano Via Molino 3-I-20079 S.Angelo Lodigiano (MI) Italy Max-Planck-Institut für Züchtungsforschung Carl-von-Linné-Weg 10 D-50829 Köln-Vogelsang Germany Abstract To study the relationships between different species of the Einkorn group, 55 different accessions of Triticum monococcum , T. boeoticum , T. urartu , T. sinskajae , T. thaoudar and T. aegilopoides were analyzed. Fifteen anonymous probes and four clones corresponding to storage protein genes were used for detecting restriction fragment length polymorphisms (RFLPs). The DNA was restricted with the restriction enzymes Alu I, Hae III, Rsa I and Taq I. The 25 probe/enzyme combinations employed yielded a total of 488 polymorphic fragments. Statistical analyses were performed using Jaccard's coefficient of similarity and principal coordinate analysis. Different values of similarity within the three main taxa, monococcum , boeoticum and urartu , were obtained; the grouping at the species level was quite well reflected by the RFLP analysis done here. The coincidence between RFLP data and the subspecies classification of the T. monococcum group was only partial. One T. urartu accession is clearly different from all of the other 54 accessions. The need for an RFLP based revision of the Einkorn taxonomy is evident. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png TAG Theoretical and Applied Genetics Springer Journals

RFLP-based genetic relationships of Einkorn wheats

Loading next page...
 
/lp/springer-journals/rflp-based-genetic-relationships-of-einkorn-wheats-eqtRnOlhXj

References (20)

Publisher
Springer Journals
Copyright
Copyright © 1994 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Agriculture; Biochemistry, general; Plant Biochemistry; Plant Sciences; Plant Genetics & Genomics
ISSN
0040-5752
eISSN
1432-2242
DOI
10.1007/BF01253991
pmid
24186183
Publisher site
See Article on Publisher Site

Abstract

122 88 88 6 7 R. Castagna G. Maga M. Perenzin M. Heun F. Salamini Istituto Sperimentale per la Cerealicoltura Sezione di S.Angelo Lodigiano Via Molino 3-I-20079 S.Angelo Lodigiano (MI) Italy Max-Planck-Institut für Züchtungsforschung Carl-von-Linné-Weg 10 D-50829 Köln-Vogelsang Germany Abstract To study the relationships between different species of the Einkorn group, 55 different accessions of Triticum monococcum , T. boeoticum , T. urartu , T. sinskajae , T. thaoudar and T. aegilopoides were analyzed. Fifteen anonymous probes and four clones corresponding to storage protein genes were used for detecting restriction fragment length polymorphisms (RFLPs). The DNA was restricted with the restriction enzymes Alu I, Hae III, Rsa I and Taq I. The 25 probe/enzyme combinations employed yielded a total of 488 polymorphic fragments. Statistical analyses were performed using Jaccard's coefficient of similarity and principal coordinate analysis. Different values of similarity within the three main taxa, monococcum , boeoticum and urartu , were obtained; the grouping at the species level was quite well reflected by the RFLP analysis done here. The coincidence between RFLP data and the subspecies classification of the T. monococcum group was only partial. One T. urartu accession is clearly different from all of the other 54 accessions. The need for an RFLP based revision of the Einkorn taxonomy is evident.

Journal

TAG Theoretical and Applied GeneticsSpringer Journals

Published: Aug 1, 1994

There are no references for this article.