Access the full text.
Sign up today, get DeepDyve free for 14 days.
K. Çiftçi, R. Levy (2001)
Enhanced plasmid DNA transfection with lysosomotropic agents in cultured fibroblasts.International journal of pharmaceutics, 218 1-2
Danuta Balicki, Ralph Reisfeld, U. Pertl, Ernest Beutler, Holger Lode (2000)
Histone H2A-mediated transient cytokine gene delivery induces efficient antitumor responses in murine neuroblastoma.Proceedings of the National Academy of Sciences of the United States of America, 97 21
T. Bieber, W. Meissner, S. Kostin, A. Niemann, Hans-Peter Elsasser (2002)
Intracellular route and transcriptional competence of polyethylenimine-DNA complexes.Journal of controlled release : official journal of the Controlled Release Society, 82 2-3
A. Ziady, T. Ferkol, D. Dawson, D. Perlmutter, P. Davis (1999)
Chain Length of the Polylysine in Receptor-targeted Gene Transfer Complexes Affects Duration of Reporter Gene Expression Both in Vitro and in Vivo *The Journal of Biological Chemistry, 274
E. Check (2002)
Gene therapy: A tragic setbackNature, 420
H. Petersen, Klaus Kunath, Alison Martin, S. Stolnik, C. Roberts, M. Davies, T. Kissel (2002)
Star-shaped poly(ethylene glycol)-block-polyethylenimine copolymers enhance DNA condensation of low molecular weight polyethylenimines.Biomacromolecules, 3 5
Michèle Lecocq, S. Coninck, Nathanael Laurent, R. Wattiaux, M. Jadot (2000)
Uptake and intracellular fate of polyethylenimine in vivo.Biochemical and biophysical research communications, 278 2
D. Esser, H. Amanuma, A. Yoshiki, M. Kusakabe, R. Rudolph, G. Böhm (2000)
A hyperthermostable bacterial histone-like protein as an efficient mediator for transfection of eukaryotic cellsNature Biotechnology, 18
K. Clark, P. Johnson (2001)
Gene delivery of vaccines for infectious disease.Current opinion in molecular therapeutics, 3 4
O. Keil, H. Bojar, H. Prisack, P. Dall (2001)
Novel lipophilic chloroquine analogues for a highly efficient gene transfer into gynecological tumors.Bioorganic & medicinal chemistry letters, 11 19
R. Kircheis, T. Blessing, S. Brunner, L. Wightman, E. Wagner (2001)
Tumor targeting with surface-shielded ligand--polycation DNA complexes.Journal of controlled release : official journal of the Controlled Release Society, 72 1-3
D. Schaffer, N. Fidelman, N. Dan, D. Lauffenburger (2000)
Vector unpacking as a potential barrier for receptor-mediated polyplex gene delivery.Biotechnology and bioengineering, 67 5
I. Zuhorn, V. Oberle, W. Visser, J. Engberts, U. Bakowsky, E. Polushkin, D. Hoekstra (2002)
Phase behavior of cationic amphiphiles and their mixtures with helper lipid influences lipoplex shape, DNA translocation, and transfection efficiency.Biophysical journal, 83 4
C. Gebhart, A. Kabanov (2001)
Evaluation of polyplexes as gene transfer agents.Journal of controlled release : official journal of the Controlled Release Society, 73 2-3
George Wu, C. Wu (1988)
Receptor-mediated gene delivery and expression in vivo.The Journal of biological chemistry, 263 29
D. Fischer, T. Bieber, S. Brüsselbach, H. Elsässer, T. Kissel (2001)
Cationized human serum albumin as a non-viral vector system for gene delivery? Characterization of complex formation with plasmid DNA and transfection efficiency.International journal of pharmaceutics, 225 1-2
David Dunlap, Alessia Maggi, Marco Soria, Lucia Monaco (1997)
Nanoscopic structure of DNA condensed for gene delivery.Nucleic acids research, 25 15
R. Mumper, Alain Rolland (1998)
Plasmid delivery to muscle: Recent advances in polymer delivery systems.Advanced drug delivery reviews, 30 1-3
S. Diebold, H. Lehrmann, M. Kursa, E. Wagner, M. Cotten, M. Zenke (1999)
Efficient gene delivery into human dendritic cells by adenovirus polyethylenimine and mannose polyethylenimine transfection.Human gene therapy, 10 5
D. Simberg, D. Danino, Y. Talmon, A. Minsky, M. Ferrari, C. Wheeler, Y. Barenholz (2001)
Phase Behavior, DNA Ordering, and Size Instability of Cationic LipoplexesThe Journal of Biological Chemistry, 276
L. Qin, D. Pahud, Y. Ding, A. Bielinska, J. Kukowska-Latallo, J. Baker, J. Bromberg (1998)
Efficient transfer of genes into murine cardiac grafts by Starburst polyamidoamine dendrimers.Human gene therapy, 9 4
R. Kircheis, Susanne Schüller, S. Brunner, M. Ogris, K. Heider, W. Zauner, E. Wagner (1999)
Polycation‐based DNA complexes for tumor‐targeted gene delivery in vivoThe Journal of Gene Medicine, 1
Regis Cartier, Regina Reszka (2002)
Utilization of synthetic peptides containing nuclear localization signals for nonviral gene transfer systemsGene Therapy, 9
P. Erbacher, J. Remy, J. Behr (1999)
Gene transfer with synthetic virus-like particles via the integrin-mediated endocytosis pathwayGene Therapy, 6
Y. Lim, Seon-mi Kim, H. Suh, Jong-sang Park (2002)
Biodegradable, endosome disruptive, and cationic network-type polymer as a highly efficient and nontoxic gene delivery carrier.Bioconjugate chemistry, 13 5
M. Zanta, O. Boussif, and Adib, J. Behr (1997)
In vitro gene delivery to hepatocytes with galactosylated polyethylenimine.Bioconjugate chemistry, 8 6
W. Henner, Ingrid Kleber, R. Benzinger (1973)
Transfection of Escherichia coli Spheroplasts III. Facilitation of Transfection and Stabilization of Spheroplasts by Different Basic PolymersJournal of Virology, 12
E. Marshall (1999)
Gene Therapy Death Prompts Review of Adenovirus VectorScience, 286
R. Fraley, S. Subramani, P. Berg, D. Papahadjopoulos (1980)
Introduction of liposome-encapsulated SV40 DNA into cells.The Journal of biological chemistry, 255 21
Ge Liu, M. Molas, G. Grossmann, M. Pasumarthy, J. Perales, M. Cooper, R. Hanson (2001)
Biological Properties of Poly-l-lysine-DNA Complexes Generated by Cooperative Binding of the Polycation* 210The Journal of Biological Chemistry, 276
David Lynn, Dustin Anderson, David Putnam, Robert Langer (2001)
Accelerated discovery of synthetic transfection vectors: parallel synthesis and screening of a degradable polymer library.Journal of the American Chemical Society, 123 33
Sang-Oh Han, Ram Mahato, Sung Kim (2001)
Water-soluble lipopolymer for gene delivery.Bioconjugate chemistry, 12 3
D. Dean, Brenda Dean, S. Muller, L. Smith (1999)
Sequence requirements for plasmid nuclear import.Experimental cell research, 253 2
J. Luzio, B. Mullock, P. Pryor, M. Lindsay, D. James, R. Piper (2001)
Relationship between endosomes and lysosomes.Biochemical Society transactions, 29 Pt 4
Hélène Pollard, J. Remy, G. Loussouarn, S. Demolombe, J. Behr, D. Escande (1998)
Polyethylenimine but Not Cationic Lipids Promotes Transgene Delivery to the Nucleus in Mammalian Cells*The Journal of Biological Chemistry, 273
S. Paul, B. Calmels, R. Acres (2002)
Improvement of adoptive cellular immunotherapy of human cancer using ex-vivo gene transfer.Current gene therapy, 2 1
W. Godbey, K. Wu, A. Mikos (1999)
Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery.Proceedings of the National Academy of Sciences of the United States of America, 96 9
C. Pouton, P. Lucas, Beverley Thomas, A. Uduehi, D. Milroy, S. Moss (1998)
Polycation-DNA complexes for gene delivery: a comparison of the biopharmaceutical properties of cationic polypeptides and cationic lipids.Journal of controlled release : official journal of the Controlled Release Society, 53 1-3
R. Kircheis, A. Kichler, G. Wallner, M. Kursa, M. Ogris, T. Felzmann, M. Buchberger, E. Wagner, E. Wagner (1997)
Coupling of cell-binding ligands to polyethylenimine for targeted gene deliveryGene Therapy, 4
P. Wadhwa, S. Zielske, Justin Roth, C. Ballas, J. Bowman, S. Gerson (2002)
Cancer gene therapy: scientific basis.Annual review of medicine, 53
Robert Lee, Leaf Huang (1997)
Lipidic vector systems for gene transfer.Critical reviews in therapeutic drug carrier systems, 14 2
J. Vacik, Brenda Dean, Warren Zimmer, David Dean (1999)
Cell-specific nuclear import of plasmid DNAGene Therapy, 6
H. Phillips (2002)
Genes can come true.New scientist, 176 2371
Mini Thomas, A. Klibanov (2002)
Enhancing polyethylenimine's delivery of plasmid DNA into mammalian cellsProceedings of the National Academy of Sciences of the United States of America, 99
C. Chan, D. Jans (2002)
Using nuclear targeting signals to enhance non‐viral gene transferImmunology and Cell Biology, 80
R. Golan, L. Pietrasanta, W. Hsieh, H. Hansma (1999)
DNA toroids: stages in condensation.Biochemistry, 38 42
W. Godbey, M. Barry, M. Barry, P. Saggau, K. Wu, A. Mikos (2000)
Poly(ethylenimine)-mediated transfection: a new paradigm for gene delivery.Journal of biomedical materials research, 51 3
O. Boussif, F. Lezoualc’h, M. Zanta, M. Mergny, D. Scherman, B. Demeneix, J. Behr (1995)
A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine.Proceedings of the National Academy of Sciences of the United States of America, 92 16
J. S̆misterová, A. Wagenaar, M. Stuart, E. Polushkin, G. Brinke, R. Hulst, J. Engberts, D. Hoekstra (2001)
Molecular Shape of the Cationic Lipid Controls the Structure of Cationic Lipid/Dioleylphosphatidylethanolamine-DNA Complexes and the Efficiency of Gene Delivery*The Journal of Biological Chemistry, 276
N. Boyce (2001)
Trial halted after gene shows up in semenNature, 414
D. Matulis, I. Rouzina, V. Bloomfield (2002)
Thermodynamics of cationic lipid binding to DNA and DNA condensation: roles of electrostatics and hydrophobicity.Journal of the American Chemical Society, 124 25
F. Burrows, M. Gore, W. Smiley, M. Kanemitsu, D. Jolly, S. Read, Thomas Nicholas, C. Kruse (2002)
Purified herpes simplex virus thymidine kinase retroviral particles:Cancer Gene Therapy, 9
S. Brunner, Elke Fürtbauer, T. Sauer, M. Kursa, E. Wagner (2002)
Overcoming the nuclear barrier: cell cycle independent nonviral gene transfer with linear polyethylenimine or electroporation.Molecular therapy : the journal of the American Society of Gene Therapy, 5 1
G. Borchard (2001)
Chitosans for gene delivery.Advanced drug delivery reviews, 52 2
M. Woodle, P. Scaria (2001)
Cationic liposomes and nucleic acidsCurrent Opinion in Colloid and Interface Science, 6
J. Dennig, Emma Duncan (2002)
Gene transfer into eukaryotic cells using activated polyamidoamine dendrimers.Journal of biotechnology, 90 3-4
Sek Hui, Marek Langner, Ya-Li Zhao, P. Ross, Edward Hurley, Karen Chan (1996)
The role of helper lipids in cationic liposome-mediated gene transfer.Biophysical journal, 71 2
V. Tendeloo, C. Broeckhoven, Z. Berneman (2001)
Gene-based cancer vaccines: an ex vivo approachLeukemia, 15
M. Wolfert, L. Seymour (1996)
Atomic force microscopic analysis of the influence of the molecular weight of poly(L)lysine on the size of polyelectrolyte complexes formed with DNA.Gene therapy, 3 3
T. Merdan, Klaus Kunath, Dagmar Fischer, Jindřich Kopeček, T. Kissel (2002)
Intracellular Processing of Poly(Ethylene Imine)/Ribozyme Complexes Can Be Observed in Living Cells by Using Confocal Laser Scanning Microscopy and Inhibitor ExperimentsPharmaceutical Research, 19
M. Ehrlich, L. Sarafyan, D. Myers (1976)
Interaction of microbial DNA with cultured mammalian cells. Binding of the donor DNA to the cell surface.Biochimica et biophysica acta, 454 3
C. Pichon, C. Gonçalves, P. Midoux (2001)
Histidine-rich peptides and polymers for nucleic acids delivery.Advanced drug delivery reviews, 53 1
I. Moret, José Peris, V. Guillem, M. Benet, F. Revert, F. Dasí, A. Crespo, S. Aliño (2001)
Stability of PEI-DNA and DOTAP-DNA complexes: effect of alkaline pH, heparin and serum.Journal of controlled release : official journal of the Controlled Release Society, 76 1-2
B. Zinselmeyer, S. Mackay, A. Schatzlein, I. Uchegbu (2002)
The Lower-Generation Polypropylenimine Dendrimers Are Effective Gene-Transfer AgentsPharmaceutical Research, 19
K. Weld, Brant Mayher, J. Allay, Jody Cockroft, Christopher Reed, M. Randolph, Yi Lu, M. Steiner, J. Gingrich (2002)
Transrectal gene therapy of the prostate in the canine modelCancer Gene Therapy, 9
B. Metcalfe, K. Sellers, M. Jeng, M. Huentelman, M. Katovich, M. Raizada (2001)
Gene therapy for cardiovascular disorders: is there a future?Annals of the New York Academy of Sciences, 953
S. Brunner, T. Sauer, S. Carotta, M. Cotten, M. Saltik, E. Wagner (2000)
Cell cycle dependence of gene transfer by lipoplex, polyplex and recombinant adenovirusGene Therapy, 7
Y. Lim, Y. Choi, Jong-sang Park (1999)
A self-destroying polycationic polymer: Biodegradable poly(4-hydroxy-L- proline ester)Journal of the American Chemical Society, 121
Gene therapy, i.e., the expression in cells of genetic material with therapeutic activity, holds great promise for the treatment of human diseases. A delivery vehicle (vector), of either viral or non-viral origin, must be used to carry the foreign gene into a cell. Viral vectors take advantage of the facile integration of the gene of interest into the host and high probability of its long-term expression but are plagued by safety concerns. Non-viral vectors, although less efficient at introducing and maintaining foreign gene expression, have the profound advantage of being non-pathogenic and non-immunogenic; they are the subject of this review. Polycation–DNA complexes are particularly attractive for non-viral gene therapy. To perform, they have to attach to the target cell surface, be internalized, escape from endosomes, find a way to the nucleus, and, finally, be available for transcription. The clinical usefulness of polycationic vectors depends on elucidating the role each of these steps plays in gene transfer. Recent progress in consequent rational vector improvement is highlighted by our finding of polyethylenimine derivatives more potent and yet less cytotoxic than the 25-kDa polyethylenimine (one of the most effective non-viral vectors). Such vectors could be further modified with cell-targeting ligands to enhance their utility for in vivo applications.
Applied Microbiology and Biotechnology – Springer Journals
Published: Apr 29, 2003
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.