Access the full text.
Sign up today, get DeepDyve free for 14 days.
Seidman
(1987)
Sequence of the left end of Mu
S. Scofield, C. Tobias, J. Rathjen, Jeff Chang, Daniel Lavelle, R. Michelmore, B. Staskawicz (1996)
Molecular Basis of Gene-for-Gene Specificity in Bacterial Speck Disease of TomatoScience, 274
J. Leach, F. White (1996)
Bacterial avirulence genes.Annual review of phytopathology, 34
U. Bonas (1994)
hrp genes of phytopathogenic bacteria.Current topics in microbiology and immunology, 192
(1993)
Pseudomonas syringae pv. syringae harpinPss: a protein that is secreted via the Hrp pathway and elicits the hypersensitive response in plant
A. Collmer (1996)
Bacterial avirulence proteins: Where's the action?Trends in Plant Science, 1
M. Tharaud, M. Menggad, J. Paulin, J. Laurent (1994)
Virulence, growth, and surface characteristics of Erwinia amylovora mutants with altered pathogenicityMicrobiology, 140
M. Barny, M. Guinebretière, B. Marçais, Eric Coissac, Jean-Pierre Paulin, J. Laurent (1990)
Cloning of a large gene cluster involved in Erwinia amylovora CFBP1430 virulenceMolecular Microbiology, 4
D. Kobayashi, S. Tamaki, N. Keen (1989)
Cloned avirulence genes from the tomato pathogen Pseudomonas syringae pv. tomato confer cultivar specificity on soybean.Proceedings of the National Academy of Sciences of the United States of America, 86 1
L. Rahme, M. Mindrinos, N. Panopoulos (1992)
Plant and environmental sensory signals control the expression of hrp genes in Pseudomonas syringae pv. phaseolicolaJournal of Bacteriology, 174
B. Castilho, '. Olfson, Malcolm CASADABANl (1984)
Plasmid insertion mutagenesis and lac gene fusion with mini-mu bacteriophage transposonsJournal of Bacteriology, 158
A. Vivian, M. Gibbon (1997)
Avirulence genes in plant-pathogenic bacteria: signals or weapons?Microbiology, 143 3
K. Wengelnik, U. Bonas (1996)
HrpXv, an AraC-type regulator, activates expression of five of the six loci in the hrp cluster of Xanthomonas campestris pv. vesicatoriaJournal of Bacteriology, 178
(1988)
Structure and function of bacterial s factors
Yingxian Xiao, S. Heu, J. Yi, Y. Lu, S. Hutcheson (1994)
Identification of a putative alternate sigma factor and characterization of a multicomponent regulatory cascade controlling the expression of Pseudomonas syringae pv. syringae Pss61 hrp and hrmA genesJournal of Bacteriology, 176
G. Ackerveken, E. Marois, U. Bonas (1996)
Recognition of the Bacterial Avirulence Protein AvrBs3 Occurs inside the Host Plant CellCell, 87
Yingxian Xiao, Y. Lu, S. Heu, S. Hutcheson (1992)
Organization and environmental regulation of the Pseudomonas syringae pv. syringae 61 hrp clusterJournal of Bacteriology, 174
E. Steinberger, S. Beer (1988)
Creation and complementation of pathogenicity mutants of Erwinia amylovoraMolecular Plant-microbe Interactions, 1
K. Wengelnik, G. vandenAckerveken, U. Bonas (1996)
HrpG, a key hrp regulatory protein of Xanthomonas campestris pv. vesicatoria is homologous to two-component response regulators.Molecular plant-microbe interactions : MPMI, 9 8
M. Arlat, F. Gijsegem, J. Huet, J. Pernollet, C. Boucher (1994)
PopA1, a protein which induces a hypersensitivity‐like response on specific Petunia genotypes, is secreted via the Hrp pathway of Pseudomonas solanacearum.The EMBO Journal, 13
Scott Stachell, G. An, C. Flores, Eugene Nesterl (1985)
A Tn3 lacZ transposon for the random generation of beta‐galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium.The EMBO Journal, 4
(1994)
A single promoter sequence recognized by a newly identified s factor directs expression of pathogenicity and host range determinants in Pseudomonas syringae
G. Cornelis, H. Wolf‐Watz (1997)
The Yersinia Yop virulon: a bacterial system for subverting eukaryotic cellsMolecular Microbiology, 23
Xiaoyan Tang, R. Frederick, Jianmin Zhou, D. Halterman, Yulin Jia, G. Martin (1996)
Initiation of Plant Disease Resistance by Physical Interaction of AvrPto and Pto KinaseScience, 274
Jeffrey Miller (1972)
Experiments in molecular genetics
(1987)
Current Protocols in Molecular Biology
J. Alfano, A. Collmer (1996)
Bacterial Pathogens in Plants: Life up against the Wall.The Plant cell, 8
N. Bardonnet, C. Blanco (1992)
'uidA-antibiotic-resistance cassettes for insertion mutagenesis, gene fusions and genetic constructions.FEMS microbiology letters, 72 3
P. Gill, G. Warren (1988)
An iron-antagonized fungistatic agent that is not required for iron assimilation from a fluorescent rhizosphere pseudomonadJournal of Bacteriology, 170
E. Roine, Wensheng Wei, Jing Yuan, E. Nurmiaho‐Lassila, N. Kalkkinen, M. Romantschuk, S. He (1997)
Hrp pilus: an hrp-dependent bacterial surface appendage produced by Pseudomonas syringae pv. tomato DC3000.Proceedings of the National Academy of Sciences of the United States of America, 94 7
C. Schoedel, A. Collmer (1986)
Evidence of homology between the pectate lyase-encoding pelB and pelC genes in Erwinia chrysanthemiJournal of Bacteriology, 167
T. Zwet, L. Harry (1979)
Fire blight, a bacterial disease of Rosaceous plants.
D. Bauer, S. Beer (1991)
Further characterization of an hrp gene cluster of Erwinia amylovoraMolecular Plant-microbe Interactions, 4
(1993)
Evolutionary conservation of pathogenicity determinants among plant and animal pathogenic bacteria
Zhong-Min Wei, S. Beer (1993)
HrpI of Erwinia amylovora functions in secretion of harpin and is a member of a new protein familyJournal of Bacteriology, 175
K. Walters, Ali Maroofi, E. Hitchin, J. Mansfield (1990)
Gene for pathogenicity and ability to cause the hypersensitive reaction cloned from Erwinia amylovora.Physiological and Molecular Plant Pathology, 36
K. Apel, K. Kloppstech (1978)
The plastid membranes of barley (Hordeum vulgare). Light-induced appearance of mRNA coding for the apoprotein of the light-harvesting chlorophyll a/b protein.European journal of biochemistry, 85 2
Z. Klement (1963)
Rapid Detection of the Pathogenicity of Phytopathogenic PseudomonadsNature, 199
J. Lorang, N. Keen (1995)
Characterization of avrE from Pseudomonas syringae pv. tomato: a hrp-linked avirulence locus consisting of at least two transcriptional units.Molecular plant-microbe interactions : MPMI, 8 1
M. Arlat, Clare Gough, C. Zischek, P. Barberis, André Trigalet, C. Boucher (1992)
Transcriptional organization and expression of the large hrp gene cluster of Pseudomonas solanacearum.Molecular plant-microbe interactions : MPMI, 5 2
R. Schulte, U. Bonas (1992)
A Xanthomonas Pathogenicity Locus Is Induced by Sucrose and Sulfur-Containing Amino Acids.The Plant cell, 4
Thanh Huynh, D. Dahlbeck, B. Staskawicz (1989)
Bacterial blight of soybean: regulation of a pathogen gene determining host cultivar specificity.Science, 245 4924
P. Wattiau, S. Woestyn, G. Cornelis (1996)
Customized secretion chaperones in pathogenic bacteriaMolecular Microbiology, 20
T. Franza, D. Expert (1991)
The virulence-associated chrysobactin iron uptake system of Erwinia chrysanthemi 3937 involves an operon encoding transport and biosynthetic functionsJournal of Bacteriology, 173
Suresh Gopalan, D. Bauer, J. Alfano, A. Loniello, S. He, A. Collmer (1996)
Expression of the Pseudomonas syringae avirulence protein AvrB in plant cells alleviates its dependence on the hypersensitive response and pathogenicity (Hrp) secretion system in eliciting genotype-specific hypersensitive cell death.The Plant cell, 8
(1973)
Le feu bactérien en
(1920)
Le feu bacté rien en france II. Caractè res des souches d'Erwinia amylovora (Burril)
Jihyun Kim, Zhong-Min Wei, S. Beer (1997)
The hrpA and hrpC operons of Erwinia amylovora encode components of a type III pathway that secretes harpinJournal of Bacteriology, 179
Zhong-Min Wei, S. Beer (1995)
hrpL activates Erwinia amylovora hrp gene transcription and is a member of the ECF subfamily of sigma factorsJournal of Bacteriology, 177
J. Lorang, Hao Shen, D. Kobayashi, D. Cooksey, N. Keen (1994)
avrA and avrE in Pseudomonas syringae pv. tomato PT23 play a role in virulence on tomato plantsMolecular Plant-microbe Interactions, 7
(1920)
isolées du foyer franco-belge
Catherine Lee (1997)
Type III secretion systems: machines to deliver bacterial proteins into eukaryotic cells?Trends in microbiology, 5 4
Zhong-Min Wei, B. Sneath, S. Beer (1992)
Expression of Erwinia amylovora hrp genes in response to environmental stimuliJournal of Bacteriology, 174
Zhong-Min Wei, R. Laby, C. Zumoff, D. Bauer, S. He, A. Collmer, S. Beer (1992)
Harpin, elicitor of the hypersensitive response produced by the plant pathogen Erwinia amylovora.Science, 257 5066
S. Genin, C. Gough, C. Zischek, C. Boucher (1992)
Evidence that the hrpB gene encodes a positive regulator of pathogenicity genes from Pseudomonas solanacearumMolecular Microbiology, 6
A. Bogdanove, Z. Wei, L. Zhao, S. Beer (1996)
Erwinia amylovora secretes harpin via a type III pathway and contains a homolog of yopN of Yersinia sppJournal of Bacteriology, 178
In Erwinia amylovora, the dsp region, required for pathogenicity on the host plant but not for hypersensitive elicitation on tobacco, is separated from the hrp region by 4 kb. The genetic analysis reported in this paper showed that this 4 kb region is not required for pathogenicity on pear seedlings. The environmental conditions allowing expression of a dsp::lacZ fusion were examined: expression was barely detected in rich medium at 30°C, and the highest expression was observed in M9 galactose minimal medium at 25°C. A dsp::uidA fusion appeared to be expressed only in a HrpL‐proficient strain, indicating that the dsp region, like the hrp region, is positively controlled via the alternative σ factor HrpL. Sequence analysis revealed that the dsp cluster encodes two genes, dspA (5517 bp) and dspB (420 bp), and that the insertions leading to the dsp::lacZ and the dsp::uidA fusions were within dspA. A HrpL‐dependent promoter sequence (GGAACC‐ N15‐CAACA) was identified upstream of dspA, and primer extension analysis detected four transcriptional starts 7, 8, 9 and 10 bp downstream of this sequence. A σ70 promoter sequence (TTGCCC‐N16‐GATAAT) was observed upstream of dspB. The functionality of this second promoter was confirmed by complementation analysis. This promoter allowed constitutive expression of dspB, as measured by the expression of a dspB::uidA fusion in rich medium. In M9 galactose medium, however, HrpL was shown to activate dspB, as expression of the dspB::uidA fusion was twofold higher in a HrpL+ background than in a HrpL− background. Transposon insertions in either dspA or dspB led to a non‐pathogenic phenotype. Thus, both DspA and DspB were required for E. amylovora pathogenicity, as dspB could be expressed independently of dspA. DspA and DspB were visualized as polypeptides with apparent sizes of 190 kDa and 15.5 kDa, respectively, when encoded in the T7 polymerase/promoter system. DspA, which showed homology with the protein predicted from the partial sequence of Pseudomonas syringae pv. tomato avrE transcriptional unit III, was shown to be secreted into the external medium via the Hrp secretion pathway. DspB was predicted to be acidic, like the Syc chaperone of Yersinia. A chaperone role for DspB was suggested further by the fact that DspA secretion required a functional DspB protein.
Molecular Microbiology – Wiley
Published: Dec 1, 1997
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.